scholarly journals Rapid drug-susceptibility testing of Mycobacterium tuberculosis clinical isolates to first-line antitubercular drugs by nitrate reductase assay: A comparison with proportion method

2016 ◽  
Vol 5 (4) ◽  
pp. 469-474 ◽  
Author(s):  
Amrish Kohli ◽  
Gulnaz Bashir ◽  
Akeela Fatima ◽  
Abiroo Jan ◽  
Nayeem-u-din Wani ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Nitrate Reductase ◽  
Susceptibility Testing ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Clinical Isolates ◽  
First Line ◽  
Antitubercular Drugs ◽  
Nitrate Reductase Assay ◽  
Proportion Method

scholarly journals Rapid Identification and Drug Susceptibility Testing of Mycobacterium tuberculosis: Standard Operating Procedure for Non- Commercial Assays: Part 2: Nitrate Reductase Assay v1.3.12

2012 ◽  
Vol 4 (02) ◽  
pp. 112-119 ◽  
Author(s):  
Sarman Singh ◽  
Parveen Kumar ◽  
Shreya Sharma ◽  
Francis Mumbowa ◽  
Anandi Martin ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Nitrate Reductase ◽  
Operating Procedure ◽  
Susceptibility Testing ◽  
Growth Yield ◽  
Standard Operating Procedure ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Standard Operating ◽  
Nitrate Reductase Assay

ABSTRACTIn the previous part, we presented the standard operating procedure (SOP) of the microscopic observation drug susceptibility assay drug susceptibility testing (DST) for Mycobacterium tuberculosis. The present SOP is devoted to another non-commercial culture and DST method known as nitrate reductase assay (NRA). As the name implies, the NRA detects the ability of M. tuberculosis to reduce nitrate to nitrite. In the assay, the presence of nitrite is detected by the addition of p-nitrobenzoate into the growth yield. The reaction is detected by the naked eye. The incorporation of drugs in the medium allows to use the test for DST, which can be interpreted with naked eyes. The identification and drug susceptibility results can be obtained in 2-3 weeks. This SOP document has been developed through the culture and DST subgroup of the STOP tuberculosis (TB) Partnership New Diagnostic Working Group. It is intended for laboratories that would want to use or already using this rapid non-commercial method for culture identification and DST of M. tuberculosis, notably in resource-constraint settings in Asia and Africa.

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