A novel recombinant Leishmania donovani p45, a partial coding region of methionine aminopeptidase, generates protective immunity by inducing a Th1 stimulatory response against experimental visceral leishmaniasis

2012 ◽  
Vol 42 (5) ◽  
pp. 429-435 ◽  
Author(s):  
Reema Gupta ◽  
Pramod K. Kushawaha ◽  
Chandra Dev Pati Tripathi ◽  
Shyam Sundar ◽  
Anuradha Dube
Keyword(s):  
Visceral Leishmaniasis ◽  
Protective Immunity ◽  
Leishmania Donovani ◽  
Methionine Aminopeptidase ◽  
Coding Region

scholarly journals Experimental visceral leishmaniasis in high and low antibody - producer mice (selection IV-A)

1999 ◽  
Vol 32 (3) ◽  
pp. 229-234 ◽  
Author(s):  
Cristiane Jellmayer Fechio ◽  
Angela Maria Victoriano de Campos Soares ◽  
Silvio Luís de Oliveira ◽  
Alexandrina Sartori
Keyword(s):  
Visceral Leishmaniasis ◽  
Protective Immunity ◽  
Macrophage Activation ◽  
Leishmania Donovani ◽  
Parasite Burden ◽  
Parasite Infection ◽  
High Antibody ◽  
Splenic Cells ◽  
Splenic Index ◽  
Direct Counts

Leishmaniasis is a typical parasite infection whose protective immunity depends on macrophage activation. Susceptibility to Leishmania donovani infection was compared in H (high antibody responder) and L (low antibody responder) mice from selection IV-A. H mice infected intravenously with 10(7) amastigotes of L. donovani were more susceptible to infection than their L counterparts. This higher susceptibility was characterized by a higher splenic and hepatic parasite burden. An increased splenic index was observed in both lines after sixty days of infection. This splenomegaly was caused, at least partially, by an increase in the number of splenic cells as determined by direct counts of cells from spleen. The results show that selection IV-A is susceptible to visceral leishmaniasis, with the H line being more susceptible than the L line.

scholarly journals Intracellular Replication-Deficient Leishmania donovani Induces Long Lasting Protective Immunity against Visceral Leishmaniasis

2009 ◽  
Vol 183 (3) ◽  
pp. 1813-1820 ◽  
Author(s):  
Angamuthu Selvapandiyan ◽  
Ranadhir Dey ◽  
Susanne Nylen ◽  
Robert Duncan ◽  
David Sacks ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Protective Immunity ◽  
Leishmania Donovani ◽  
Intracellular Replication

scholarly journals Characterization of Leishmania donovani Antigens Encapsulated in Liposomes That Induce Protective Immunity in BALB/c Mice

2002 ◽  
Vol 70 (12) ◽  
pp. 6697-6706 ◽  
Author(s):  
Farhat Afrin ◽  
Ravindran Rajesh ◽  
Khairul Anam ◽  
Meenakshisundram Gopinath ◽  
Swati Pal ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Protective Immunity ◽  
Leishmania Donovani ◽  
Delayed Type Hypersensitivity ◽  
Striking Similarity ◽  
Antigen Profile ◽  
Membrane Antigens ◽  
The Right ◽  
Positively Charged

ABSTRACT Leishmania donovani promastigote membrane antigens (LAg) encapsulated in positively charged liposomes have been found to induce very significant levels of protection against experimental visceral leishmaniasis. The protectively immunized animals exhibited profound delayed-type hypersensitivity and antibody responses. The extent of protection induced by the same antigens, however, varied depending on the charge of the vesicles, with maximum induction by positively charged liposomes, followed by neutral liposomes and last negatively charged liposomes. Characterization of LAg and LAg entrapped in liposomes of different charges by Western blot analysis revealed the immunodominance of gp63 in all three vaccine preparations. The strong reactivity of antigens in a restricted antigen profile that included, in addition to gp63, 72-, 52-, 48-, 45-, 39-, and 20-kDa components in neutral and positively charged liposomes contrasted with the reactivity of a greater number of LAg components in negatively charged liposomes. Resistance to visceral leishmaniasis appears to depend on the immunity induced by gp63 and a few select antigens in association with the right liposomes. A striking similarity between the immunogenic profile of partially purified soluble antigens and that of LAg in neutral and positively charged liposomes suggests the potentiality of these antigens in future vaccine studies of L. donovani.

Immune induction by adjuvanted Leishmania donovani vaccines against the visceral leishmaniasis in BALB/c mice

Immunobiology ◽  
2021 ◽  
Vol 226 (2) ◽  
pp. 152057
Author(s):  
Deepak Kumar Goyal ◽  
Poonam Keshav ◽  
Sukhbir Kaur
Keyword(s):  
Visceral Leishmaniasis ◽  
Leishmania Donovani

scholarly journals Clinical and laboratory profiles of visceral leishmaniasis among adult patients admitted to Felege Hiwot Hospital, Bahir Dar, Ethiopia

2021 ◽  
Vol 9 ◽  
pp. 205031212110367
Author(s):  
Berhanu Tarekegn ◽  
Ayanaw Tamene
Keyword(s):  
Visceral Leishmaniasis ◽  
Leishmania Donovani ◽  
Clinical Presentation ◽  
Winter Season ◽  
Adult Patients ◽  
Sand Fly ◽  
Timely Initiation ◽  
Vector Borne Disease ◽  
Vector Borne ◽  
Endemic Areas

Background: Visceral leishmaniasis is a vector-borne disease caused by Leishmania donovani transmitted by sand fly species. It is the third most common vector-borne disease globally. Visceral leishmaniasis is endemic in Ethiopia with an estimated annual incidence ranging from 3700 to 7400 cases. This research aimed to assess the clinical presentations and laboratory profiles of visceral leishmaniasis for early diagnosis and timely initiation of management. Objective: To describe the clinical and laboratory manifestation and diagnostic modalities of visceral leishmaniasis among adult patients admitted to Felege Hiwot Hospital, from 1 September 2016 to 30 August 2019. Method: Institution-based retrospective cross-sectional study was conducted among 141 patients admitted to Felege Hiwot Hospital from 1 September 2016 to 30 August 2019. Descriptive statistics were used to describe the clinical presentation and laboratory profiles of patients with visceral leishmaniasis. Results: Among a total of 141 enrolled patients in the study, males were affected 13-fold. Most of them were travelers to endemic areas during the winter season for labor work. The mean duration of illness was 48 days. Common symptoms were fever (96.5%), weightless (82.5%), jaundice (18.4%), vomiting/diarrhea (13.5%), and bleeding episodes (11.3%). Splenomegaly was seen in 98.6%, ascites in 35.5%, and lymphadenopathy in 9.9%. Lymphadenopathy was seen significantly in HIV patients (40%). Anemia was seen in 95%, thrombocytopenia in 90.2%, leukopenia in 86.4%, and pancytopenia in 79.4%. Half of the patients had coinfection. Neutropenic sepsis was seen in 21.3%. The diagnosis was made by tissue aspiration in 65% of patients. Conclusion: The majority of patients who were diagnosed to have visceral leishmaniasis were young male adults who traveled to the endemic areas seasonally. Fever and splenomegaly were seen as the commonest clinical presentation. Lymphadenopathy occurred in high frequency among HIV co-infected patients. Anemia was the commonest hematologic finding.

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