A polymerized hemoglobin based oxygen carrier attenuates TLR4 stimulated NF-kB activation by inhibiting phosphorylation of IkB-a

2005 ◽  
Vol 201 (3) ◽  
pp. S34-S35
Author(s):  
Aaron M. Cheng ◽  
Ernest E. Moore ◽  
Jeffrey Johnson ◽  
Mark Walsh ◽  
Christine Hamiel ◽  
...  
Keyword(s):  
Oxygen Carrier ◽  
Polymerized Hemoglobin

Controlled Polymerization and Ultrafiltration Increase the Consistency of Polymerized Hemoglobin for Use as an Oxygen Carrier

2019 ◽  
Vol 31 (3) ◽  
pp. 605-621 ◽  
Author(s):  
Donald A. Belcher ◽  
Clayton T. Cuddington ◽  
Evan L. Martindale ◽  
Ivan S. Pires ◽  
Andre F. Palmer
Keyword(s):  
Oxygen Carrier ◽  
Controlled Polymerization ◽  
Polymerized Hemoglobin

scholarly journals Mechanism of Interference of a Polymerized Hemoglobin Blood Substitute in an Alkaline Phosphatase Method

2000 ◽  
Vol 46 (9) ◽  
pp. 1331-1337 ◽  
Author(s):  
Jeffrey J Chance ◽  
Edward J Norris ◽  
Martin H Kroll
Keyword(s):  
Alkaline Phosphatase ◽  
Oxygen Carrier ◽  
Blood Substitute ◽  
Polymerization Process ◽  
Oxygen Carriers ◽  
Alkaline Phosphatase Assay ◽  
Analytical Wavelength ◽  
Phosphatase Assay ◽  
Interference Mechanism ◽  
Polymerized Hemoglobin

Abstract Background: Hemoglobin-based oxygen carriers can cause profound interferences in many analytical procedures. We determined the mechanism of interference in the assay of alkaline phosphatase activity and identified approaches that might be used to correct for this interference. Methods: Interference of a polymerized hemoglobin blood substitute with the assay of alkaline phosphatase was examined with a Hitachi 917 analyzer and ultraviolet-visible spectrophotometry. Results: Hemoglobin-based oxygen carrier solutions had substantial absorbance at 415 nm, the wavelength of analysis used to measure the formation of 4-nitrophenol. In addition to offsetting the initial absorbance at the analytical wavelength, polymerized hemoglobin gave rise to a strong negative interference plot because of alkali denaturation of the substitute. The same interference mechanism was also observed for native hemoglobin (hemolysate), indicating that the interference was not derived from the polymerization process. The interference can be corrected by implementing a rate-correction procedure, or the interference can be avoided by measurement at 450 nm. Conclusions: The interference of polymerized hemoglobin in the alkaline phosphatase assay is a result of an absorbance offset caused by alkali denaturation of hemoglobin. The interference can be corrected or avoided by modifying the calculation or the analytical wavelength. The correction strategy may also be applicable to improving the hemolysis index for this method.

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