Small-angle X-ray scattering: a high-throughput technique for investigating archaeological bone preservation

2004 ◽  
Vol 31 (10) ◽  
pp. 1349-1359 ◽  
Author(s):  
J.C Hiller ◽  
M.J Collins ◽  
A.T Chamberlain ◽  
T.J Wess
Keyword(s):  
High Throughput ◽  
Small Angle ◽  
X Ray ◽  
X Ray Scattering ◽  
Bone Preservation ◽  
Ray Scattering

scholarly journals Automated microfluidic sample-preparation platform for high-throughput structural investigation of proteins by small-angle X-ray scattering

2011 ◽  
Vol 44 (5) ◽  
pp. 1090-1099 ◽  
Author(s):  
Josiane P. Lafleur ◽  
Detlef Snakenborg ◽  
Søren S. Nielsen ◽  
Magda Møller ◽  
Katrine N. Toft ◽  
...  
Keyword(s):  
Sample Preparation ◽  
High Throughput ◽  
Small Angle ◽  
Structural Investigation ◽  
Adaptor Protein ◽  
Complete Analysis ◽  
Uv Absorbance ◽  
X Ray ◽  
X Ray Scattering ◽  
Ray Scattering

A new microfluidic sample-preparation system is presented for the structural investigation of proteins using small-angle X-ray scattering (SAXS) at synchrotrons. The system includes hardware and software features for precise fluidic control, sample mixing by diffusion, automated X-ray exposure control, UV absorbance measurements and automated data analysis. As little as 15 µl of sample is required to perform a complete analysis cycle, including sample mixing, SAXS measurement, continuous UV absorbance measurements, and cleaning of the channels and X-ray cell with buffer. The complete analysis cycle can be performed in less than 3 min. Bovine serum albumin was used as a model protein to characterize the mixing efficiency and sample consumption of the system. The N2 fragment of an adaptor protein (p120-RasGAP) was used to demonstrate how the device can be used to survey the structural space of a protein by screening a wide set of conditions using high-throughput techniques.

scholarly journals Small angle X-ray scattering as a high-throughput method to classify antimicrobial modes of action

2016 ◽  
Vol 1858 (5) ◽  
pp. 918-925 ◽  
Author(s):  
A.R. von Gundlach ◽  
V.M. Garamus ◽  
T. Gorniak ◽  
H.A. Davies ◽  
M. Reischl ◽  
...  
Keyword(s):  
High Throughput ◽  
Small Angle ◽  
X Ray ◽  
Modes Of Action ◽  
X Ray Scattering ◽  
High Throughput Method ◽  
Ray Scattering

scholarly journals Characterization of lipid matrices for membrane protein crystallization by high-throughput small angle X-ray scattering

Methods ◽  
2011 ◽  
Vol 55 (4) ◽  
pp. 342-349 ◽  
Author(s):  
Jeremiah S. Joseph ◽  
Wei Liu ◽  
Joshua Kunken ◽  
Thomas M. Weiss ◽  
Hiro Tsuruta ◽  
...  
Keyword(s):  
Membrane Protein ◽  
High Throughput ◽  
Small Angle ◽  
Protein Crystallization ◽  
X Ray ◽  
X Ray Scattering ◽  
Membrane Protein Crystallization ◽  
Ray Scattering

scholarly journals In situ capabilities of Small Angle X-ray Scattering

2019 ◽  
Vol 8 (1) ◽  
pp. 352-369 ◽  
Author(s):  
Jinghua Feng ◽  
Manfred Kriechbaum ◽  
Li (Emily) Liu
Keyword(s):  
Magnetic Field ◽  
High Throughput ◽  
Small Angle ◽  
Nanoscale Systems ◽  
X Ray ◽  
X Ray Scattering ◽  
Flow Through ◽  
Stretching Flow ◽  
Ray Scattering

Abstract Small Angle X-ray Scattering (SAXS) is an ideal characterization tool to explore nanoscale systems. In order to investigate nanostructural changes of materials under realistic sample environments, it is essential to equip SAXS with diverse in situ capabilities based on the corresponding requirements. In this paper, we highlight the representative experimental setups and corresponding applications of five widely used in situ capabilities: temperature, pressure, stretching, flow-through, and electric field. Additionally, we also briefly introduce other four in situ techniques including humidity, high-throughput, rheology, and magnetic field.

High-Throughput Small Angle X-ray Scattering from Proteins in Solution Using a Microfluidic Front-End

2008 ◽  
Vol 80 (10) ◽  
pp. 3648-3654 ◽  
Author(s):  
K. Nørgaard Toft ◽  
Bente Vestergaard ◽  
Søren S. Nielsen ◽  
Detlef Snakenborg ◽  
Mads G. Jeppesen ◽  
...  
Keyword(s):  
High Throughput ◽  
Small Angle ◽  
X Ray ◽  
X Ray Scattering ◽  
Front End ◽  
Ray Scattering

scholarly journals A General Small-Angle X-ray Scattering-Based Screening Protocol for Studying Physical Stability of Protein Formulations

Pharmaceutics ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 69
Author(s):  
Fangrong Zhang ◽  
Gesa Richter ◽  
Benjamin Bourgeois ◽  
Emil Spreitzer ◽  
Armin Moser ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
High Throughput ◽  
Small Angle ◽  
X Ray ◽  
Sensitive Measure ◽  
X Ray Scattering ◽  
Protein Formulations ◽  
Protein Particle ◽  
Ray Scattering

A fundamental step in developing a protein drug is the selection of a stable storage formulation that ensures efficacy of the drug and inhibits physiochemical degradation or aggregation. Here, we designed and evaluated a general workflow for screening of protein formulations based on small-angle X-ray scattering (SAXS). Our SAXS pipeline combines automated sample handling, temperature control, and fast data analysis and provides protein particle interaction information. SAXS, together with different methods including turbidity analysis, dynamic light scattering (DLS), and SDS-PAGE measurements, were used to obtain different parameters to provide high throughput screenings. Using a set of model proteins and biopharmaceuticals, we show that SAXS is complementary to dynamic light scattering (DLS), which is widely used in biopharmaceutical research and industry. We found that, compared to DLS, SAXS can provide a more sensitive measure for protein particle interactions, such as protein aggregation and repulsion. Moreover, we show that SAXS is compatible with a broader range of buffers, excipients, and protein concentrations and that in situ SAXS provides a sensitive measure for long-term protein stability. This workflow can enable future high-throughput analysis of proteins and biopharmaceuticals and can be integrated with well-established complementary physicochemical analysis pipelines in (biopharmaceutical) research and industry.

scholarly journals Robust, high-throughput solution structural analyses by small angle X-ray scattering (SAXS)

2009 ◽  
Vol 6 (8) ◽  
pp. 606-612 ◽  
Author(s):  
Greg L Hura ◽  
Angeli L Menon ◽  
Michal Hammel ◽  
Robert P Rambo ◽  
Farris L Poole II ◽  
...  
Keyword(s):  
High Throughput ◽  
Small Angle ◽  
X Ray ◽  
X Ray Scattering ◽  
Ray Scattering

scholarly journals Coupling High Throughput Microfluidics and Small-Angle X-ray Scattering to Study Protein Crystallization from Solution

2017 ◽  
Vol 89 (4) ◽  
pp. 2282-2287 ◽  
Author(s):  
Nhat Pham ◽  
Dimitri Radajewski ◽  
Adam Round ◽  
Martha Brennich ◽  
Petra Pernot ◽  
...  
Keyword(s):  
High Throughput ◽  
Small Angle ◽  
Protein Crystallization ◽  
X Ray ◽  
X Ray Scattering ◽  
Crystallization From Solution ◽  
Ray Scattering
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