Mutational Analysis of Biliary Duct Brushings and Pancreatic Fine Needle Aspirates in Residual Supernatant Fluid after Cell Block Preparation: Is There Diagnostic Utility?

2014 ◽  
Vol 3 (5) ◽  
pp. S25
Author(s):  
Jordan Roberts ◽  
Maren Fuller ◽  
Nicole Nelles ◽  
Hidehiro Takei
Keyword(s):  
Mutational Analysis ◽  
Biliary Duct ◽  
Diagnostic Utility ◽  
Cell Block ◽  
Supernatant Fluid ◽  
Fine Needle ◽  
Fine Needle Aspirates

The diagnostic utility of cell block in fine needle aspiration cytology of palpable breast lesions in a Nigerian tertiary health institution

2020 ◽  
Vol 48 (12) ◽  
pp. 1300-1306
Author(s):  
Fred John Nnaemeka Obiajulu ◽  
Adetola Olubunmi Daramola ◽  
Charles Chidozie Anunobi ◽  
Nzechukwu Zimudo Ikeri ◽  
Fatimah Biade Abdulkareem ◽  
...  
Keyword(s):  
Fine Needle Aspiration ◽  
Fine Needle Aspiration Cytology ◽  
Needle Aspiration ◽  
Breast Lesions ◽  
Diagnostic Utility ◽  
Cell Block ◽  
Aspiration Cytology ◽  
Fine Needle ◽  
Palpable Breast ◽  
Needle Aspiration Cytology

Biliary Dilation: An Unusual Presentation of Histoplasma capsulatum in a Dog

2020 ◽  
Vol 56 (5) ◽  
pp. 285
Author(s):  
Harry Cridge ◽  
Erin Brinkman ◽  
Patty Lathan
Keyword(s):  
Histoplasma Capsulatum ◽  
Abdominal Distension ◽  
Abdominal Ultrasound ◽  
Biliary Duct ◽  
Potential Complication ◽  
Intact Male ◽  
Fine Needle ◽  
Fine Needle Aspirates ◽  
Disseminated Histoplasmosis ◽  
History Of

ABSTRACT A 4 mo old intact male Labrador retriever was referred to the authors’ institution for a 3 wk history of weight loss and progressive abdominal distension. Thoracic radiographs revealed sternal lymphadenopathy and a diffuse unstructured interstitial and bronchial pulmonary pattern. An abdominal ultrasound revealed multifocal lymphadenopathy, hepatosplenomegaly, and biliary duct dilation. Fine-needle aspirates of the spleen and liver revealed Histoplasma capsulatum organisms. Antifungal therapy was initiated with itraconazole and terbinafine. Repeat ultrasound examinations revealed resolving hepatosplenomegaly and resolving cystic duct dilation. Ultrasonographic findings mirrored a decrease in urine H capsulatum antigen levels. Histoplasmosis should be considered as a differential for unexplained biliary duct dilation or obstruction in dogs. Dogs with disseminated histoplasmosis should be monitored for this potential complication.

scholarly journals Diagnostic Utility of Cell Block Technique as an Adjunct Technique to Cytological Smears in Evaluation of Thyroid Aspirates on Fine Needle Aspiration Cytology

1969 ◽  
Vol 11 (4) ◽  
pp. 217-222
Author(s):  
Amreek Lal ◽  
Siyab Ahmad ◽  
Waseem Khan ◽  
Liaqat Ali ◽  
Mian Anwar Zeb ◽  
...  
Keyword(s):  
Informed Consent ◽  
Fine Needle Aspiration ◽  
Fine Needle Aspiration Cytology ◽  
Needle Aspiration ◽  
Diagnostic Utility ◽  
Cell Block ◽  
Aspiration Cytology ◽  
Fine Needle ◽  
Significant Difference ◽  
Needle Aspiration Cytology

Background: Fine needle aspiration cytology is a standard screening technique for the diagnosis and assessment of thyroidnodules. However, this approach has some drawbacks, such as false negative or positive outcomes and high rates ofunsatisfactory results.Objective: To describe the utility of cell block technique as an adjunct to conventional smear in the cytodiagnosis of thyroidepithelial lesions.Material and Methods: This Descriptive Cross-sectional study was done at the Pathology department of Saidu Group of TeachingHospital Saidu Sharif Swat from 1st November 2017 to 31st April 2018. Fine needle aspirates for both the direct smear and cell blockwere collected simultaneously from 60 patients, through non- probability convenient sampling. All patients having clinicallyenlarged thyroid, solitary or dominant thyroid nodule were included in the study.All patients below 22 years old and patients who didnot give informed consent were excluded. Ethical clearance was obtained from the ethical research committee before carrying outthe study. Written informed consent was taken from all the participating patients.Cellularity, obscuring background material, morphological and architectural preservation as well as cytodiagnosis on direct smearand cell block technique were compared. Data were analyzed using SPSS software version 21. McNemar's chi-square test wasapplied for significant difference. P-value of = 0.05 was considered significant.Results: Male to Female ratio was 1:7.5, (13%: 87%). The age ranged from 22 to 81 years with a mean age of 44.9 and standarddeviation of ±14.5 years. The study showed that the cell block technique has high cellularity, minimal obscuring background, betterarchitectural preservation and poor morphological preservation on cell block in comparison to direct smear. The majority of thecases were benign on both techniques. The unsatisfactory rate was reduced in cell block technique and showed additionalinformation in 13 % cases.Conclusion: The study demonstrated no significant difference in the diagnostic utility of both techniques.Keywords: Cell Block Cytopathology, FNAC, Mair et al criteria

scholarly journals Novel Modification of HistoGel-Based Cell Block Preparation Method: Improved Sufficiency for Molecular Studies

2017 ◽  
Vol 142 (4) ◽  
pp. 529-535 ◽  
Author(s):  
Natasha Rekhtman ◽  
Darren J. Buonocore ◽  
Dorota Rudomina ◽  
Maria Friedlander ◽  
Crisbane Dsouza ◽  
...  
Keyword(s):  
Preparation Method ◽  
Ex Vivo ◽  
Substantial Improvement ◽  
New Method ◽  
Cell Block ◽  
Cell Capture ◽  
Preparation Methods ◽  
Fine Needle ◽  
Fine Needle Aspirates ◽  
Molecular Studies

Context.— Cell block preparation methods vary substantially across institutions and are frequently suboptimal. The growing importance of biomarker testing in the era of targeted therapies makes optimization of cell block preparation critically important. Objective.— To develop an improved cell block preparation method. Design.— Ex vivo fine-needle aspirates and scrapes from surgically resected tumors were used to develop an improved HistoGel (Thermo Fisher Scientific, Waltham, Massachusetts)-based cell block preparation method. Cellularity yield with the new versus the standard method was assessed in ex vivo split samples and in consecutive clinical fine-needle aspirates processed before (n = 100) and after (n = 100) the new method was implemented in our laboratory. Sufficiency of cell block material for potential molecular studies was estimated by manual cell quantitation. Results.— The key modification in the new method was pretreatment of the pelleted cells with 95% ethanol before the addition of HistoGel (HistoGel + ethanol method). In addition, we optimized the melting conditions of HistoGel and added a dark, inorganic marker to the cell pellets to highlight the desired level of sectioning during microtomy. Cell blocks from ex vivo split samples showed that the HistoGel + ethanol method yielded, on average, an 8.3-fold (range, 1–20) greater cellularity compared with the standard HistoGel-only method. After the switch from the standard HistoGel method to the modified method in our clinical practice, sufficiency of positive fine-needle aspirates for some molecular studies increased from 72% to 97% (P = .002). Conclusions.— We describe a simple and readily adoptable modification of the HistoGel method, which results in substantial improvement in cell capture in cell blocks, leading to a significant increase in sufficiency for potential molecular and other ancillary studies.

BRAF Mutational Analysis on Fine Needle Aspirates of Metastatic Melanoma: A Cytology-Histology Correlation Study

2012 ◽  
Vol 1 (1) ◽  
pp. S121
Author(s):  
Kurt Bernacki ◽  
Bryan Betz ◽  
Helmut Weigelin ◽  
Christopher Lao ◽  
Bruce Redman ◽  
...  
Keyword(s):  
Metastatic Melanoma ◽  
Mutational Analysis ◽  
Correlation Study ◽  
Fine Needle ◽  
Fine Needle Aspirates
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