Isolation of a high malic and low acetic acid-producing sake yeast Saccharomyces cerevisiae strain screened from respiratory inhibitor 2,4-dinitrophenol (DNP)-resistant strains

2014 ◽  
Vol 117 (1) ◽  
pp. 39-44 ◽  
Author(s):  
Shingo Kosugi ◽  
Keiji Kiyoshi ◽  
Takahiro Oba ◽  
Kenichi Kusumoto ◽  
Toshimori Kadokura ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acetic Acid ◽  
Yeast Saccharomyces Cerevisiae ◽  
Respiratory Inhibitor ◽  
Resistant Strains

scholarly journals Unique genetic basis of the distinct antibiotic potency of high acetic acid production in the probiotic yeast Saccharomyces cerevisiae var. boulardii

2019 ◽  
Vol 29 (9) ◽  
pp. 1478-1494 ◽  
Author(s):  
Benjamin Offei ◽  
Paul Vandecruys ◽  
Stijn De Graeve ◽  
María R. Foulquié-Moreno ◽  
Johan M. Thevelein
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acetic Acid ◽  
Acid Production ◽  
Genetic Basis ◽  
Yeast Saccharomyces Cerevisiae ◽  
Acetic Acid Production ◽  
Probiotic Yeast

scholarly journals INHIBITION OF THE GROWTH OF TOLERANT YEAST Saccharomyces cerevisiae STRAIN I136 BY A MIXTURE OF SYNTHETIC INHIBITORS

2017 ◽  
Vol 18 (1) ◽  
pp. 17
Author(s):  
Eny Ida Riyanti ◽  
Edy Listanto
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acetic Acid ◽  
Carbon Sources ◽  
Resistance Mechanism ◽  
Lag Phase ◽  
Cell Factory ◽  
Yeast Saccharomyces Cerevisiae ◽  
Fermentation Products ◽  
Hydroxymethyl Furfural ◽  
Cell Biomass

<p>Biomass from lignocellulosic wastes is a potential source for biobased products.  However, one of the constraints in utilization of biomass hydrolysate is the presence of inhibitors. Therefore, the use of inhibitor-tolerant microorganisms in the fermentation is required. The study aimed to investigate the effect of a mixture of inhibitors on the growth of Saccharomyces cerevisiae strain I136 grown in medium containing synthetic inhibitors (acetic acid, formic acid, furfural, 5-hydroxymethyl furfural/5-HMF, and levulinic acid) in four different concentrations with a mixture of carbon sources, glucose  (50 g.l-1) and xylose (50 g.l-1) at 30oC. The parameters related to growth and fermentation products were observed. Results showed that the strain was able to grow in media containing natural inhibitors (BSL medium) with µmax of 0.020/h. Higher level of synthetic inhibitors prolonged the lag phase, decreased the cell biomass and ethanol production, and specific growth rate. The strain could detoxify furfural and 5-HMF and produced the highest ethanol (Y(p/s) of 0.32 g.g-1) when grown in BSL. Glucose was utilized as its level decreased in a result of increase in cell biomass, in contrast to xylose which was not consumed. The highest cell biomass was produced in YNB with Y (x/s) value of 0.25 g.g-1. The strain produced acetic acid as a dominant side product and could convert furfural into a less toxic compound, hydroxyl furfural. This robust tolerant strain provides basic information on resistance mechanism and would be useful for bio-based cell factory using lignocellulosic materials. </p>

The Resistance of Yeast Saccharomyces Cerevisiae to Extreme Conditions

2021 ◽  
pp. 113-118
Author(s):  
Elvira A. Islammagomedova ◽  
Eslanda A. Khalilova ◽  
Rasul Z. Gasanov ◽  
Aida A. Abakarova ◽  
Dinara A. Aliverdieva
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Extreme Values ◽  
Maximum Size ◽  
Surface Color ◽  
Yeast Saccharomyces Cerevisiae ◽  
Wide Range ◽  
Extreme Factors ◽  
Alkaline Conditions ◽  
Biotechnological Processes ◽  
Resistant Strains

The resistance of the yeast Saccharomyces cerevisiae DAW-3а and Y-503 to the conditions of extreme values of pH, NaCl, temperature has been studied. Under different cultivation modes, the rounded shape of DAW-3a cells is pre-served and this parameter Y-503 changes. Under conditions of different pH values and 30°C, the maximum sizes of cells and giant colonies of the polyploid Y-503 strain were found in comparison with the haploid DAW-3a; at 37°C, the advantage of Y-503 was not observed and the colony sizes of both strains were practically the same. The reaction of the strains to critical concentrations of NaCl in the medium was identical: a decrease in the size of cells and colonies was found; change in the shape, surface, color and structure of colonies. Under conditions of the simultaneous influence of an elevated temperature of 37°C, a wide range of pH and 5 % NaCl, the cell size decreased slightly; under neutral and alkaline conditions of cultivation, a slightly greater tolerance of yeast to salt stress was established; a decrease in the size of giant colonies was found, while the maximum size of the studied strains was noted at pH 11.0, the minimum at pH 3.0. The study of the tolerance of the yeast S. cerevisiae DAW-3a and Y-503 to extreme factors is of particular interest in connection with the possibility of using stress-resistant strains in biotechnological processes.

scholarly journals Polygenic analysis and targeted improvement of the complex trait of high acetic acid tolerance in the yeast Saccharomyces cerevisiae

2016 ◽  
Vol 9 (1) ◽  
Author(s):  
Jean-Paul Meijnen ◽  
Paola Randazzo ◽  
María R. Foulquié-Moreno ◽  
Joost van den Brink ◽  
Paul Vandecruys ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acetic Acid ◽  
Acid Tolerance ◽  
Complex Trait ◽  
Acetic Acid Tolerance ◽  
Yeast Saccharomyces Cerevisiae ◽  
Polygenic Analysis

Methylated sterols from polyene-resistant strains of the yeast Saccharomyces cerevisiae

1987 ◽  
Vol 23 (4) ◽  
pp. 514-515
Author(s):  
Z. A. Zhakovskaya ◽  
T. E. Ogorodnikova ◽  
N. P. Mikhailova ◽  
K. A. V'yunov
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Yeast Saccharomyces Cerevisiae ◽  
Resistant Strains

scholarly journals Improvement of inhibitor tolerance in Saccharomyces cerevisiae by overexpression of the quinone oxidoreductase family gene YCR102C

2019 ◽  
Vol 19 (6) ◽  
Author(s):  
Hongqi Chen ◽  
Jie Li ◽  
Chun Wan ◽  
Qing Fang ◽  
Fengwu Bai ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acetic Acid ◽  
Acid Stress ◽  
Acid Tolerance ◽  
Cell Factory ◽  
Quinone Oxidoreductase ◽  
Yeast Saccharomyces Cerevisiae ◽  
Inhibitor Tolerance ◽  
Family Gene ◽  
Novel Strategy

ABSTRACT Budding yeast Saccharomyces cerevisiae is widely used for lignocellulosic biorefinery. However, its fermentation efficiency is challenged by various inhibitors (e.g. weak acids, furfural) in the lignocellulosic hydrolysate, and acetic acid is commonly present as a major inhibitor. The effects of oxidoreductases on the inhibitor tolerance of S. cerevisiae have mainly focused on furfural and vanillin, whereas the influence of quinone oxidoreductase on acetic acid tolerance is still unknown. In this study, we show that overexpression of a quinone oxidoreductase-encoding gene, YCR102C, in S. cerevisiae, significantly enhanced ethanol production under acetic acid stress as well as in the inhibitor mixture, and also improved resistance to simultaneous stress of 40°C and 3.6 g/L acetic acid. Increased catalase activities, NADH/NAD+ ratio and contents of several metals, especially potassium, were observed by YCR102C overexpression under acetic acid stress. To our knowledge, this is the first report that the quinone oxidoreductase family protein is related to acid stress tolerance. Our study provides a novel strategy to increase lignocellulosic biorefinery efficiency using yeast cell factory.

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