Aqueous two-phase partitioning and characterization of xylanase produced by Streptomyces geysiriensis from low cost lignocellulosic substrates

2020 ◽  
Vol 130 (6) ◽  
pp. 571-576
Author(s):  
Shanmugam Poornima ◽  
Palanisamy Divya ◽  
Natchimuthu Karmegam ◽  
Vivekanandhan Karthik ◽  
Ramasamy Subbaiya
Keyword(s):  
Low Cost ◽  
Two Phase ◽  
Phase Partitioning ◽  
Lignocellulosic Substrates

Two-phase partitioning and partial characterization of a collagenase from Penicillium aurantiogriseum URM4622: Application to collagen hydrolysis

2013 ◽  
Vol 75 ◽  
pp. 64-71 ◽  
Author(s):  
Carolina A. Lima ◽  
Augusto C.V. Freitas Júnior ◽  
José L. Lima Filho ◽  
Attilio Converti ◽  
Daniela A. Viana Marques ◽  
...  
Keyword(s):  
Partial Characterization ◽  
Two Phase ◽  
Phase Partitioning ◽  
Penicillium Aurantiogriseum

scholarly journals EXTRACTION OF COLLAGENOLYTIC ENZYME FROM FISH VISCERA BY PHASE PARTITIONING (PEG/CITRATE) AND ITS POTENTIAL FOR INDUSTRIAL APPLICATION

2021 ◽  
Vol 46 (4) ◽  
pp. 1-8
Author(s):  
Jessica Costa SILVA ◽  
Luiz Henrique Svintiskas LINO ◽  
Márcia Nieves CARNEIRO DA CUNHA ◽  
Juanize Matias da Silva BATISTA ◽  
Vagne Melo OLIVEIRA ◽  
...  
Keyword(s):  
Low Cost ◽  
Aqueous System ◽  
Added Value ◽  
Collagenolytic Activity ◽  
Two Phase ◽  
Phase Partitioning ◽  
Centropomus Undecimalis ◽  
Common Snook ◽  
Extraction And Purification ◽  
Fish Viscera

Internal viscera fish are potential sources of protein biomolecules of biopharmaceutical interest. However, this residue is frequently discarded inappropriately. The possibility to obtain by products of higher added value is a reality. Inside this view attention must be given to processes for the recovery and extraction of target molecules. However, the high cost of processing these residues is one of the obstacles to their reuse; techniques that facilitate their handling and make the process cheaper are desirable, such as extraction in a two-phase aqueous system. Thus, the aim of this study was to extract collagenolytic enzymes from common snook (Centropomus undecimalis) using a two-phase aqueous system (polyethylene glycol/citrate), according to the 24 factorial design, using as variables: molar mass of PEG (MPEG), PEG concentration (CPEG), citrate concentration (CCIT), pH, still, considering purification factor (FP), partition coefficient (K), and yield (Y). The collagenolytic activity of the crude extract was 102.41 U mg-1, after partitioning, was purified 3.91 times (MPEG: 8000; CPEG: 20.0%; CCIT: 20.0% and pH 6.0). Inhibition (U mg-1) was observed in benzamidine (22.51), TLCK (21.05), TPCK (21.29), and PMSF (23.05), signaling to be a serine-protease. The results showed the advantage of this semipurification technique as concerns to the low cost of extraction and purification, adding value to the fishing source material and allocating the residues from its processing to the industry.

Characterization of pH dependence in organic acid absorption with non-reactive and reactive polymers for application in two-phase partitioning bioreactors

2016 ◽  
Vol 287 ◽  
pp. 503-510 ◽  
Author(s):  
Eric C. Peterson ◽  
David Contreras-López ◽  
Jesse Harris ◽  
J. Scott Parent ◽  
Andrew J. Daugulis
Keyword(s):  
Organic Acid ◽  
Ph Dependence ◽  
Two Phase ◽  
Phase Partitioning ◽  
Acid Absorption ◽  
Reactive Polymers
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