BILLFISH CATCHES FROM OCEANIC RECREATIONAL FISHING IN THE ROYAL CHARLOTTE BANK, BAHIA, BRAZIL

The aim of this study was to analyze the oceanic recreational fishing in the Royal Charlotte Bank, state of Bahia, Brazil. Only data from an oceanic recreational fishing operator based in the municipality of Canavieiras, southern Bahia, were used, from 2012 to 2017. Overall, 103 fish specimens were caught and released, during this period, belonging to the following species: Makaira nigricans, Kajikia albida, and Istiophorus platypterus. Fishery operations occurred from October to March, with the highest catch in number in December (56.3%). The largest annual catch was recorded in 2013, with 39 individuals. The catch per unit effort ranged from 1.0 to 1.4 fishes per trip, with the highest value in December. Even though data presented here are partial, they can help to establish a strategic scenario for the fishing sector in Bahia, along with data from other fishing sectors (artisanal, industrial, subsistence and scientific).

FEEDING HABITS OF Lutjanus synagris (TELEOSTEI: LUTJANIDAE) IN THE AMAZON COAST OF THE NORTHEAST REGION OF BRAZIL

The feeding habit of lane snapper Lutjanus synagris was analyzed qualitatively and quantitatively, related to seasonality, its ontogenetic development, and the relationship between food and biotic and abiotic conditions on the Amazon Coast of Maranhão, between June 2018 and May 2019. Numerical, gravimetric, and ecological index methods were used to show the relative importance or preference of a category or food item in the diet. The food composition was studied related to the sex, size of the predator and seasonality. Of 359 stomachs sampled, 54 were empty due to regurgitation and presented a vacuity coefficient (Cv%) of 15.04%. In the analysis of diets between the sexes, it was possible to identify a greater participation of fish (30.43%) and Crabs (26.10%) for females, Shrimp (36.23%) and Organic Matter Not Identified (OMNI) (18.84%) in males. The diet showed spatial differences in length distributions. The analysis of stomach contents showed the presence synthetic materials (mesoplastics ranging from 5.0 mm to 2.5 cm) in 5.52% of the samples. The main food items found were from the Brachyura and Caridea species. The results clearly demonstrate that lane snapper prefers benthic prey, presenting a carnivorous and generalist-opportunistic habit. Its diet is also composed of demersal-pelagic species, such as Cephalopods and Teleost fish.

EXTRACTION OF COLLAGENOLYTIC ENZYME FROM FISH VISCERA BY PHASE PARTITIONING (PEG/CITRATE) AND ITS POTENTIAL FOR INDUSTRIAL APPLICATION

Internal viscera fish are potential sources of protein biomolecules of biopharmaceutical interest. However, this residue is frequently discarded inappropriately. The possibility to obtain by products of higher added value is a reality. Inside this view attention must be given to processes for the recovery and extraction of target molecules. However, the high cost of processing these residues is one of the obstacles to their reuse; techniques that facilitate their handling and make the process cheaper are desirable, such as extraction in a two-phase aqueous system. Thus, the aim of this study was to extract collagenolytic enzymes from common snook (Centropomus undecimalis) using a two-phase aqueous system (polyethylene glycol/citrate), according to the 24 factorial design, using as variables: molar mass of PEG (MPEG), PEG concentration (CPEG), citrate concentration (CCIT), pH, still, considering purification factor (FP), partition coefficient (K), and yield (Y). The collagenolytic activity of the crude extract was 102.41 U mg-1, after partitioning, was purified 3.91 times (MPEG: 8000; CPEG: 20.0%; CCIT: 20.0% and pH 6.0). Inhibition (U mg-1) was observed in benzamidine (22.51), TLCK (21.05), TPCK (21.29), and PMSF (23.05), signaling to be a serine-protease. The results showed the advantage of this semipurification technique as concerns to the low cost of extraction and purification, adding value to the fishing source material and allocating the residues from its processing to the industry.

ICHTHYOFAUNA IN THE INNER CONTINENTAL SHELF NEXT TO THE PARANAGUÁ ESTUARINE COMPLEX, SOUTHERN BRAZIL

The composition and structure of fish assemblages in the inner continental shelf of the Paraná State are affected by cold fronts and the rainfall regime. Data from fishing activities in this region, as well as the analysis of environmental characteristics, are the main tools available for understanding fish dynamics, under influence of increasing human activities. In order to better understand patterns and temporal variations in fish assemblages in the inner continental shelf of the Paraná State, a total of 24 double trawls were performed with an otter trawl between August 2000 and July 2001, in two sampling areas, the North area in front of the northern mouth of the Paranaguá Estuary Complex, and the South area, in front of the Leste beach. A total of 45,277 fish specimens belonging to 35 families and 97 species were caught. Sciaenidae was the most abundant family, with 37.1% of the total number of individuals caught, and with the highest richness (18 species). Statistical analyses evidenced significant differences in environmental characteristics and in fish fauna, and that both areas disturbances were observed in the fish assemblages during the dry and wet season, being more intense in the Southern area, disturbances that would be related to the shrimp fishing present in the two areas sampled in this study.

ARTISANAL FISHING ON THE COAST OF ESPÍRITO SANTO STATE, SOUTHEASTERN BRAZIL: AN APPROACH TO SOCIOENVIRONMENTAL OCEANOGRAPHY

Artisanal fishing is an important traditional activity on the coast of Espírito Santo (ES) State, southeastern Brazil. Despite this, there is a lack of updated data on artisanal fishing and its interfaces with socioenvironmental oceanography. The present study describes the social profile of fishers (gender, age, education, and period of professional activity) and the fishing activity (fishing gear and target species) along the coast of Espírito Santo State. Interviews with semi-structured questionnaires were carried out with 366 artisanal fishers living in 10 fishing communities. The interviewed fishers are mostly male, with low education, who had been fishing for more than 41 years (32%). The fishers described 59 target ethnospecies, with eight being common in 90% (n = 9) of the communities under study. These workers use the following fishing gear: line, longline, gillnet, trawl net, and harpoon. Socioenvironmental oceanography enables obtaining basic information on artisanal fishing, which can support the development of public policies for the sector with a view to maintaining this activity in Espírito Santo State.

HYDROELECTRIC DAMS FROM MADEIRA RIVER SEASONALLY IMPACTS THE FISHERIES PRODUCTION IN THE GUAPORÉ BASIN (RONDÔNIA, BRAZIL)

In the Amazon region, fisheries play an important role in the socioeconomic and cultural context and are directly affected by changes in the hydrological cycle, which can interfere with the numbers and frequency of fish species landed. This study analyzed the variation of fishery production against the seasonality of the water level of the Guaporé River (a tributary of the Madeira River) using information contained in the records of fishery landings in the area under study. In addition, two periods pre (2000 to 2008) and post (2009 to 2019) installation of the Jirau and Santo Antônio dams in the Madeira River were considered. Fish production in the period prior to damming indicated linearity (r2 = 0.41) which was inversely proportional to the water levels of the Guaporé River, with low fish production in the flood and high production during the low water phases. However, for the period after damming, these variables showed low correlation (r2 = 0.14). Among the fish species exploited, 35.13% presented significant differences (p <0.05) between the production values for the periods before and after installation of the dams. Therefore, the results showed significant differences between the values of fishery production by species and phases of the hydrological cycle of the Guaporé River, which occurred in the periods pre and post damming of the Madeira River, which indicates that the hydroelectric dams have negatively impacted the fish stocks of this region. The information contained in this study is useful and serves as a basis for coherent decision-making, since it aids in the sustainable management and monitoring of fish stocks in the Madeira River Basin.

ANALYSIS OF THE ORNAMENTAL FISH EXPORTS FROM THE AMAZON STATE, BRAZIL

The updated status of the ornamental fish trade from the Amazonas state was analyzed between the years of 2006-2015 (IBAMA database). The trade of ornamental fish from Amazonas state mainly consists of species extracted from nature. A total of 142,552,253 specimens were exported during the studied period. Sales plummeted since 2006, from 26,075,241 specimens exported then to 2,729,846 specimens in 2015 (Jan-Jul). Between 2006 and 2015, a total of US$ 23.0 million in revenue was generated from fish exports destined to 35 countries. Germany, Taiwan, USA and Japan accounted for 75.5% of the volume and 76.7% of the value exported. During this time, 375 species were exported, included Paracheirodon axelrodi, P. simulans, Hemigrammus bleheri, Otociclus affinis and O. hoppei that together represented 84.5% of exports. Thirty are not on the list of IBAMA species released for export, and six are currently on the list of endangered fauna of Brazil, being them: Hopliancistrus tricornis, Leporacanthicus joselimai, Parancistrus nudiventris, Peckoltia compta, Scobinancistrus auratus and S. pariolispos. These results are an additional tool for managers, environmentalists and decision makers to establish regulations to govern the trade.

ANTIMICROBIAL ACTIVITY OF ESSENTIAL OILS OF Origanum vulgare AND Ocimum basilicum AGAINST Vibrio parahaemolyticus AND Vibrio vulnificus AND ADDITION OF THESE OILS ON Mugil platanus FILLETS

Species of the Vibrio genus are commonly reported as agents of food poisoning outbreaks associated with fish consumption. The objective of this study was to assess the antimicrobial activity of the essential oils of Origanum vulgare and Ocimum basilicum against Vibrio parahaemolyticus and Vibrio vulnificus, as well as to assess their addition to fillets of Mugil platanus and sensorial acceptance among consumers. The antimicrobial activity was analyzed by the disc diffusion test and minimal bactericidal concentration (MBC). M. platanus fillets, experimentally contaminated, were marinated for 24 hours in a solution containing 1.0% and 1.5% of essential oil of O. vulgare. For the sensory analysis, samples marinated in 1.5% of O. vulgare oil were prepared and offered to 100 testers. The oil of O. basilicum shows no antimicrobial activity, so its application would be ineffective; for such reason the subsequent tests were not performed. However, the essential oil of O. vulgare produced inhibition halo diameters ranging from 24.6 to 34.1 mm, on average, and the MBC ranging from 3.9% to 15.6%. When added to the product, the essential oil of O. vulgare eliminated the microorganisms that were experimentally inoculated. In the sensorial analysis, samples marinated in 1.5% of O. vulgare essential oil obtained an average of 6.82 of acceptance note among consumers.

ECONOMIC-FISHING ANALYSIS OF THE PRAWN Cryphiops caementarius (MOLINA, 1782) IN THE MAJES-CAMANÁ RIVER AREQUIPA-PERÚ (2019)

The prawn (Cryphiops caementarius) is the only resource in the Peruvian coastal rivers that supports a commercial fishery. Bioeconomic-fishing aspects are reported based on data (fishing, costs and income derived from fishing) acquired in situ monthly in four altitudinal strata (every 200 meters above sea level - masl) of the Majes-Camaná river during 2019. The catch per unit of effort (CPUE) was expressed in kg h-1, monthly income was estimated based on the average catch values, number of tasks and price of the resource. To evaluate the profitability of the activity of an average fisherman (by stratum), a cash flow based on income and expenses was executed under situations with a constant future, using economic profitability indicators such as the Net Present Value (NPV), Internal Rate of Return (IRR), Benefit-Cost ratio (B/C) and Discounted Payback Period (DPB). There was a predominance of the diving method, the catches (kg) and the fishing yield increased throughout the fishing period (April-December) with a maximum value of 4.8 kg h-1 (December - high strata). Investment costs per fisherman were low, roughly 1,000 soles. The sale price increases with altitude, decreasing over the course of the year depending on the availability of the resource; the monthly income per fisherman varied from 964 soles (April - low stratum) to 6,760 soles (December - high stratum). The economic simulation model showed that the income exceeded the costs of the fishing activity from the first year of activity in all the altitudinal strata, the economic profitability indicators showed high profitability for the activity.

PROTEIN REQUIREMENT FOR INITIAL REARING PHASE OF PACIFIC WHITE SHRIMP IN A BIOFLOC SYSTEM

This study evaluated the protein requirement of Litopenaeus vannamei post-larvae during the initial rearing phase in a biofloc system. Five different diets were evaluated with increasing concentrations of crude protein: 31.28, 36.29, 41.57, 46.34, and 51.74 g 100 g-1 CP. Post-larvae (0.16 ± 0.01 g) were stocked at a density of 450 PL m-3 in 400 L tanks. Water quality parameters were maintained within the limits recommended for shrimp farming. After 38 days, a regression analysis revealed that levels of CP content (65.29 – 72.83%), EE (10.45 – 11.65%) and body N (10.45 – 11.64%) increased with increasing protein levels in the diet. A similar trend was observed in the biofloc sludge with respect to CP and N. Survival exceeded 80%, and the shrimp with diets containing 31.28 to 46.34g 100 g-1 CP presented an increase in final weight (1.52 – 2.61 g), productivity (0.69 – 1.10 Kg m-3), weight gain (1.38 – 2.44 g), and feeding efficiency (77.28 – 101.68%), whereas these indices decreased to51.74 g 100 g-1 CP. Crude protein content from 44.26 to 47.12 g 100 g-1 provided the best growth performance during the initial rearing phase of Pacific white shrimp in a biofloc system.