l-Methionine sulfoximine as a novel selection agent for genetic transformation of orchids

Kanamycin is a widely used selection agent in dicot-plant genetic transformation systems. In monocots, however, it does not seem to be effective as it has no or minimal effect on the normal growth of non-transformed plants. Kanamycin was previously demonstrated to bleach the pigments of the non-transgenic plants. This may yield the idea that kanamycin can be used as an effective screening marker rather than a selectable marker in monocots.

Download Full-text

Kanamycin in Cereal Biotechnology: A Screening or a Selectable Marker?

BioScientific Review ◽

10.32350/bsr.0301.i ◽

2021 ◽

Vol 3 (1) ◽

pp. 01-05

Author(s):

Malik Shuja ◽

Hassan Riaz ◽

Muhsin Jamal ◽

Muhammad Imran

Keyword(s):

Transgenic Plants ◽

Genetic Transformation ◽

Selectable Marker ◽

Normal Growth ◽

Minimal Effect ◽

Effective Screening ◽

Transformed Plants ◽

Transformation Systems ◽

Selection Agent ◽

Screening Marker

Kanamycin is a widely used selection agent in dicot-plant genetic transformation systems. In monocots, however, it does not seem to be effective as it has no or minimal effect on the normal growth of non-transformed plants. Kanamycin was previously demonstrated to bleach the pigments of the non-transgenic plants. This may yield the idea that kanamycin can be used as an effective screening marker rather than a selectable marker in monocots. Copyright (c) 2021 Malik Nawaz Shuja, Hasan Riaz, Muhsin Jamal, Muhammad Imran

Download Full-text

Evaluation of the Phosphomannose Isomerase-Based Selection System for Genetic Transformation of Sweetpotato

International Journal of Life Sciences ◽

10.3126/ijls.v9i3.12466 ◽

2015 ◽

Vol 9 (3) ◽

pp. 46-53 ◽

Cited By ~ 3

Author(s):

Wilton Mbinda ◽

Sylvia Nawiri ◽

Makenzi Nzaro ◽

Benson Kinyagia ◽

Allan Mgutu ◽

...

Keyword(s):

Cell Growth ◽

Genetic Transformation ◽

Life Sciences ◽

Stem Explants ◽

Selection System ◽

Phosphomannose Isomerase ◽

Public Concern ◽

Effective Selection ◽

Minimal Concentration ◽

Selection Agent

In response to increased public concern on antibiotic or herbicide resistance genes usage in genetically modified plants, mannose was evaluated as selectable agent for the genetic transformation of sweetpotato. Nontransformed sweetpotato stem explants of cv. KSP36 were cultured on media containing various combinations and concentrations sucrose and mannose ranging from 0 to 30 g/l. Likewise, efficacy of hygromycin and kanamycin as selection agents for transformation of sweetpotato was evaluated on media containing varying concentrations of antibiotics ranging 0 to 100 mg/l. Selection agent effectiveness was determined by detecting the minimal concentration of the selection agent required to fully inhibit sweetpotato calli growth. Hygromycin was the most effective selection agent as it inhibited cell growth at concentrations above 20 mg/l. Kanamycin was moderately effective as it inhibited cell growth at 60 mg/l. Sweetpotato calli were able to grow and even produce embryos even when mannose was the only source of carbohydrates.DOI: http://dx.doi.org/10.3126/ijls.v9i3.12466 International Journal of Life Sciences 9 (3): 2015; 46-53

Download Full-text

Kanamycin in Cereal Biotechnology: A Screening or a Selectable Marker?

BioScientific Review ◽

10.32350/bsr/2021/31/785 ◽

2021 ◽

Vol 3 (1) ◽

pp. 01-05

Author(s):

Malik Shuja

Keyword(s):

Transgenic Plants ◽

Genetic Transformation ◽

Selectable Marker ◽

Normal Growth ◽

Minimal Effect ◽

Effective Screening ◽

Transformed Plants ◽

Transformation Systems ◽

Selection Agent ◽

Screening Marker

Kanamycin is a widely used selection agent in dicot-plant genetic transformation systems. In monocots, however, it does not seem to be effective as it has no or minimal effect on the normal growth of non-transformed plants. Kanamycin was previously demonstrated to bleach the pigments of the non-transgenic plants. This may yield the idea that kanamycin can be used as an effective screening marker rather than a selectable marker in monocots.

Download Full-text

Kanamycin in Cereal Biotechnology: A Screening or a Selectable Marker?

BioScientific Review ◽

10.32350/bsr.0301.05 ◽

2021 ◽

Vol 3 (1) ◽

pp. 01-05

Author(s):

Malik Shuja

Keyword(s):

Transgenic Plants ◽

Genetic Transformation ◽

Selectable Marker ◽

Normal Growth ◽

Minimal Effect ◽

Effective Screening ◽

Transformed Plants ◽

Transformation Systems ◽

Selection Agent ◽

Screening Marker

Kanamycin is a widely used selection agent in dicot-plant genetic transformation systems. In monocots, however, it does not seem to be effective as it has no or minimal effect on the normal growth of non-transformed plants. Kanamycin was previously demonstrated to bleach the pigments of the non-transgenic plants. This may yield the idea that kanamycin can be used as an effective screening marker rather than a selectable marker in monocots.

Download Full-text

892 PB 496 RESPONSE OF THREE JUGLANS SPP. TO KANAMYCIN AND BIALAPHOS

HortScience ◽

10.21273/hortsci.29.5.561f ◽

1994 ◽

Vol 29 (5) ◽

pp. 561f-561

Author(s):

Lynn M. Long ◽

John E. Preece ◽

Gerald R. Gaffney ◽

J.W. Van Sambeek ◽

David A. Lightfoot

Keyword(s):

Genetic Transformation ◽

Somatic Embryos ◽

Casein Hydrolysate ◽

Secondary Embryogenesis ◽

Selection Agent ◽

Cotyledonary Stage

Genetic transformation studies are aided by use of selection agents, such as antibiotics or herbicides. To determine the level of kanamycin to be used as a selection agent, cotyledonary stage somatic embryos from J. nigra lines J26 and J28, J. nigra × J. hindsii line S11, and J. regia line SU2 were placed on gelrite solidified WPM with 1 g/liter casein hydrolysate and 250 mg/liter cefotaxime and 3% (w/v) sucrose. Dosages for inhibiting secondary embryogenesis were 40 mg/liter kanamycin for J. nigra and J. nigra × J. hindsii and 100 mg/liter for J. regia. For the bialaphos experiments, somatic embryos of J. nigra lines J26 and J28 and J. nigra × J. hindsii line S11 were cultured on gelrite solidified LP medium with 0.5 g/liter casein hydrolysate and 3% (w/v) sucrose. Between 0.1 and 1.0 mg/liter bialaphos, inhibited secondary embryogenesis.

Download Full-text