scholarly journals Evaluation of the Phosphomannose Isomerase-Based Selection System for Genetic Transformation of Sweetpotato

2015 ◽  
Vol 9 (3) ◽  
pp. 46-53 ◽  
Author(s):  
Wilton Mbinda ◽  
Sylvia Nawiri ◽  
Makenzi Nzaro ◽  
Benson Kinyagia ◽  
Allan Mgutu ◽  
...  
Keyword(s):  
Cell Growth ◽  
Genetic Transformation ◽  
Life Sciences ◽  
Stem Explants ◽  
Selection System ◽  
Phosphomannose Isomerase ◽  
Public Concern ◽  
Effective Selection ◽  
Minimal Concentration ◽  
Selection Agent

In response to increased public concern on antibiotic or herbicide resistance genes usage in genetically modified plants, mannose was evaluated as selectable agent for the genetic transformation of sweetpotato. Nontransformed sweetpotato stem explants of cv. KSP36 were cultured on media containing various combinations and concentrations sucrose and mannose ranging from 0 to 30 g/l. Likewise, efficacy of hygromycin and kanamycin as selection agents for transformation of sweetpotato was evaluated on media containing varying concentrations of antibiotics ranging 0 to 100 mg/l. Selection agent effectiveness was determined by detecting the minimal concentration of the selection agent required to fully inhibit sweetpotato calli growth. Hygromycin was the most effective selection agent as it inhibited cell growth at concentrations above 20 mg/l. Kanamycin was moderately effective as it inhibited cell growth at 60 mg/l. Sweetpotato calli were able to grow and even produce embryos even when mannose was the only source of carbohydrates.DOI: http://dx.doi.org/10.3126/ijls.v9i3.12466 International Journal of Life Sciences 9 (3): 2015; 46-53

scholarly journals Phosphomannose isomerase gene for selection in lettuce (Lactuca sativa L.) transformation.

2010 ◽  
Vol 57 (1) ◽  
Author(s):  
Jindrich Bríza ◽  
Nina Růzicková ◽  
Hana Niedermeierová ◽  
Jana Dusbábková ◽  
Josef Vlasák
Keyword(s):  
Lactuca Sativa ◽  
Transcriptional Activity ◽  
Northern Analysis ◽  
Selection System ◽  
Phosphomannose Isomerase ◽  
Lactuca Sativa L ◽  
Clonal Origin ◽  
Selection Agent ◽  
Very High ◽  
Specific Mrna

A positive selection system using phosphomannose isomerase was employed for Agrobacterium tumefaciens mediated transformation of lettuce (Lactuca sativa L. var. 'Achát'). It was shown that the mannose-based selection system works very well with the lettuce genotype used, reaching up to 25% transformation efficiency on the medium with 20 g/L mannose and 20 g/L sucrose. The best transformation efficacy with the commonly-used kanamycin at 100 mg/L as a selection agent was 21%. Southern blot analyses of thirteen chosen mannose-resistant regenerants revealed that some of them have clonal origin, about one-half harbour a single T-DNA copy and one plant contains an incomplete T-DNA segment with only the left part of T-DNA with the pmi gene present in the genomic DNA. The following Northern analysis showed transcriptional activity of the introduced pmi gene in all plants analysed with very high differences in the level of pmi specific mRNA. The results demonstrate that both mannose and kanamycin provide comparable transformation efficiencies in our lettuce genotype. An alternative selection method with mannose as a selection agent is now available for lettuce transgenosis.

Genetic transformation of Torenia fournieri using the PMI/mannose selection system

2007 ◽  
Vol 90 (1) ◽  
pp. 103-109 ◽  
Author(s):  
Hong-Qing Li ◽  
Pei-Jing Kang ◽  
Mei-Lan Li ◽  
Mei-Ru Li
Keyword(s):  
Genetic Transformation ◽  
Selection System ◽  
Torenia Fournieri ◽  
Mannose Selection

Effective selection of transgenic papaya plants with the PMI/Man selection system

2005 ◽  
Vol 24 (7) ◽  
pp. 426-432 ◽  
Author(s):  
Yun J. Zhu ◽  
Ricelle Agbayani ◽  
Heather McCafferty ◽  
Henrik H. Albert ◽  
Paul H. Moore
Keyword(s):  
Selection System ◽  
Transgenic Papaya ◽  
Effective Selection ◽  
Selection Of

scholarly journals Kanamycin in Cereal Biotechnology: A Screening or a Selectable Marker?

2021 ◽  
Vol 3 (1) ◽  
pp. 01-05
Author(s):  
Malik Shuja
Keyword(s):  
Transgenic Plants ◽  
Genetic Transformation ◽  
Selectable Marker ◽  
Normal Growth ◽  
Minimal Effect ◽  
Effective Screening ◽  
Transformed Plants ◽  
Transformation Systems ◽  
Selection Agent ◽  
Screening Marker

Kanamycin is a widely used selection agent in dicot-plant genetic transformation systems. In monocots, however, it does not seem to be effective as it has no or minimal effect on the normal growth of non-transformed plants. Kanamycin was previously demonstrated to bleach the pigments of the non-transgenic plants. This may yield the idea that kanamycin can be used as an effective screening marker rather than a selectable marker in monocots.

scholarly journals Kanamycin in Cereal Biotechnology: A Screening or a Selectable Marker?

2021 ◽  
Vol 3 (1) ◽  
pp. 01-05
Author(s):  
Malik Shuja ◽  
Hassan Riaz ◽  
Muhsin Jamal ◽  
Muhammad Imran
Keyword(s):  
Transgenic Plants ◽  
Genetic Transformation ◽  
Selectable Marker ◽  
Normal Growth ◽  
Minimal Effect ◽  
Effective Screening ◽  
Transformed Plants ◽  
Transformation Systems ◽  
Selection Agent ◽  
Screening Marker

Kanamycin is a widely used selection agent in dicot-plant genetic transformation systems. In monocots, however, it does not seem to be effective as it has no or minimal effect on the normal growth of non-transformed plants. Kanamycin was previously demonstrated to bleach the pigments of the non-transgenic plants. This may yield the idea that kanamycin can be used as an effective screening marker rather than a selectable marker in monocots.   Copyright (c) 2021 Malik Nawaz Shuja, Hasan Riaz, Muhsin Jamal, Muhammad Imran

Self-assembled ZnS nanospheres with nanoscale porosity as an efficient carrier for the delivery of doxorubicin

RSC Advances ◽  
2015 ◽  
Vol 5 (112) ◽  
pp. 92499-92505
Author(s):  
Vandana Kumari ◽  
Nabanita Chatterjee ◽  
Subhadip Das ◽  
Subhajit Bhunia ◽  
Krishna Das Saha ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cell Growth ◽  
Leukemia Cell ◽  
Lymphocytic Leukemia ◽  
Delivery Vehicle ◽  
Apoptotic Pathway ◽  
Drug Delivery Vehicle ◽  
Minimal Concentration ◽  
Self Assembled ◽  
Lymphocytic Leukemia Cell

Self-aggregated mesoporous ZnS nanomaterials have been utilized as a drug-delivery vehicle that can activates the apoptotic pathway with a minimal concentration of doxorubicin and it can regulates lymphocytic leukemia cell growth.

l-Methionine sulfoximine as a novel selection agent for genetic transformation of orchids

2007 ◽  
Vol 131 (4) ◽  
pp. 466-472 ◽  
Author(s):  
Davina Chai ◽  
Sook May Lee ◽  
Jia Hui Ng ◽  
Hao Yu
Keyword(s):  
Genetic Transformation ◽  
Methionine Sulfoximine ◽  
Selection Agent

Mannose: A Potential Selection System for Genetic Transformation of Annatto

2003 ◽  
Vol 46 (3) ◽  
pp. 441-444 ◽  
Author(s):  
V.B. Neto ◽  
C.R. Carvalho ◽  
W.C. Otoni
Keyword(s):  
Genetic Transformation ◽  
Selection System
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