Cloning and characterization of the isocitrate dehydrogenase gene from oleaginous yeast Lipomyces starkeyi

2008 ◽  
Vol 136 ◽  
pp. S359-S360
Author(s):  
Wei Tang ◽  
Sufang Zhang ◽  
Haidong Tan ◽  
Zongbao K. Zhao
Keyword(s):  
Isocitrate Dehydrogenase ◽  
Oleaginous Yeast ◽  
Lipomyces Starkeyi ◽  
Dehydrogenase Gene

The isocitrate dehydrogenase gene of oleaginous yeast Lipomyces starkeyi is linked to lipid accumulation

2009 ◽  
Vol 55 (9) ◽  
pp. 1062-1069 ◽  
Author(s):  
Wei Tang ◽  
Sufang Zhang ◽  
Qian Wang ◽  
Haidong Tan ◽  
Zongbao Kent Zhao
Keyword(s):  
Isocitrate Dehydrogenase ◽  
Lipid Accumulation ◽  
Oleaginous Yeast ◽  
Phylogenetic Analyses ◽  
Dry Mass ◽  
Strain Engineering ◽  
Pcr Analysis ◽  
Lipomyces Starkeyi ◽  
Intracellular Lipids ◽  
Rapid Amplification

The oleaginous yeast Lipomyces starkeyi can accumulate intracellular lipids to over 60% of its cell dry mass under a nitrogen-limited condition. We showed that extracellular and intracellular citrate concentrations of L. starkeyi AS 2.1560 increased and the nicotinamide adenine dinucleotide – isocitrate dehydrogenase (NAD+–IDH) activity decreased at the beginning of the lipid accumulation, suggesting that the attenuation of the NAD+–IDH activity might initiate lipid storage. We next cloned the IDH gene by the methods of degenerate PCR and rapid amplification of cDNA ends. Phylogenetic analyses of the evolutionary relationships among LsIDH1, LsIDH2, and other yeast NAD+–IDHs revealed that the L. starkeyi IDH had a closer relationship with the IDHs of Yarrowia lipolytica . Further real-time PCR analysis showed that the expression levels of both LsIDH1 and LsIDH2 decreased concurrently with the evolution of cellular lipids. Our data should be valuable for understanding the biology of oleaginous yeasts and for further strain engineering of L. starkeyi.

Characterization of the mitochondrial NAD+-dependent isocitrate dehydrogenase of the oleaginous yeast Rhodosporidium toruloides

2012 ◽  
Vol 94 (4) ◽  
pp. 1095-1105 ◽  
Author(s):  
Fan Yang ◽  
Sufang Zhang ◽  
Yongjin J. Zhou ◽  
Zhiwei Zhu ◽  
Xinping Lin ◽  
...  
Keyword(s):  
Isocitrate Dehydrogenase ◽  
Oleaginous Yeast ◽  
Rhodosporidium Toruloides

Identification and characterization of Δ12 and Δ12/Δ15 bifunctional fatty acid desaturases in the oleaginous yeast Lipomyces starkeyi

2018 ◽  
Vol 102 (20) ◽  
pp. 8817-8826 ◽  
Author(s):  
Tomohiko Matsuzawa ◽  
Tomoko Maehara ◽  
Yasushi Kamisaka ◽  
Satoshi Ara ◽  
Hiroaki Takaku ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Oleaginous Yeast ◽  
Lipomyces Starkeyi ◽  
Fatty Acid Desaturases ◽  
Identification And Characterization

Characterization of a Stress-induced NADP-isocitrate Dehydrogenase Gene in Maize Confers Salt Tolerance in Arabidopsis

2010 ◽  
Vol 53 (2) ◽  
pp. 107-112 ◽  
Author(s):  
Yinghui Liu ◽  
Yunsu Shi ◽  
Yanchun Song ◽  
Tianyu Wang ◽  
Yu Li
Keyword(s):  
Salt Tolerance ◽  
Isocitrate Dehydrogenase ◽  
Dehydrogenase Gene

Cloning and characterization of an aldehyde dehydrogenase gene GmALDH3-1 from soybean

2010 ◽  
Vol 32 (5) ◽  
pp. 492-497 ◽  
Author(s):  
Fang HUANG ◽  
Ying-Jun CHI ◽  
Hui HE ◽  
De-Yue YU
Keyword(s):  
Aldehyde Dehydrogenase ◽  
Dehydrogenase Gene

Genetically Engineered Oleaginous Yeast Lipomyces starkeyi for Sesquiterpene α-Zingiberene Production

2021 ◽  
Author(s):  
Ziyu Dai ◽  
Kyle R. Pomraning ◽  
Ellen A. Panisko ◽  
Beth A. Hofstad ◽  
Kristen B. Campbell ◽  
...  
Keyword(s):  
Oleaginous Yeast ◽  
Genetically Engineered ◽  
Lipomyces Starkeyi

scholarly journals Organization and characterization of the rat class 3 aldehyde dehydrogenase gene

1993 ◽  
Vol 268 (17) ◽  
pp. 12530-12536 ◽  
Author(s):  
D.C. Asman ◽  
K. Takimoto ◽  
H.C. Pitot ◽  
T.J. Dunn ◽  
R. Lindahl
Keyword(s):  
Aldehyde Dehydrogenase ◽  
Dehydrogenase Gene
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