One-pot, three-step cascade synthesis of D-danshensu using engineered Escherichia coli whole cells

2019 ◽  
Vol 300 ◽  
pp. 48-54 ◽  
Author(s):  
Tianzhen Xiong ◽  
Pu jia ◽  
Jing Jiang ◽  
Yajun Bai ◽  
Tai-Ping Fan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
One Pot ◽  
Whole Cells ◽  
Engineered Escherichia Coli

Efficient Xylitol Production from Cornstalk Hydrolysate Using Engineered Escherichia coli Whole Cells

2018 ◽  
Vol 66 (50) ◽  
pp. 13209-13216 ◽  
Author(s):  
Ziyue Chang ◽  
Dong Liu ◽  
Zhengjiao Yang ◽  
Jinglan Wu ◽  
Wei Zhuang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Xylitol Production ◽  
Whole Cells ◽  
Engineered Escherichia Coli

scholarly journals Biosynthesis of Putrescine from L-arginine Using Engineered Escherichia coli Whole Cells

Catalysts ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 947
Author(s):  
Hongjie Hui ◽  
Yajun Bai ◽  
Tai-Ping Fan ◽  
Xiaohui Zheng ◽  
Yujie Cai
Keyword(s):  
Escherichia Coli ◽  
Biogenic Amine ◽  
Arginine Decarboxylase ◽  
Optimal Concentration ◽  
Optimum Temperature ◽  
Whole Cell ◽  
Whole Cells ◽  
Copy Numbers ◽  
Optimum Ph ◽  
Engineered Escherichia Coli

Putrescine, a biogenic amine, is a highly valued compound in medicine, industry, and agriculture. In this study, we report a whole-cell biocatalytic method in Escherichia coli for the production of putrescine, using L-arginine as the substrate. L-arginine decarboxylase and agmatine ureohydrolase were co-expressed to produce putrescine from L-arginine. Ten plasmids with different copy numbers and ordering of genes were constructed to balance the expression of the two enzymes, and the best strain was pACYCDuet-speB-speA. The optimal concentration of L-arginine was determined to be 20 mM for this strain. The optimum pH of the biotransformation was 9.5, and the optimum temperature was 45 °C; under these conditions, the yield of putrescine was 98%. This whole-cell biocatalytic method appeared to have great potential for the production of putrescine.

ChemInform Abstract: Asymmetric Baeyer-Villiger Oxidations of 4-Mono- and 4,4-Disubstituted Cyclohexanones by Whole Cells of Engineered Escherichia coli.

ChemInform ◽  
2010 ◽  
Vol 32 (27) ◽  
pp. no-no
Author(s):  
Marko D. Mihovilovic ◽  
Gang Chen ◽  
Shaozhao Wang ◽  
Brian Kyte ◽  
Fernande Rochon ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Whole Cells ◽  
Engineered Escherichia Coli

Baeyer-Villiger oxidations of representative heterocyclic ketones by whole cells of engineered Escherichia coli expressing cyclohexanone monooxygenase

2001 ◽  
Vol 11 (4-6) ◽  
pp. 349-353 ◽  
Author(s):  
Marko D. Mihovilovic ◽  
Bernhard Müller ◽  
Margaret M. Kayser ◽  
Jon D. Stewart ◽  
Johannes Fröhlich ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cyclohexanone Monooxygenase ◽  
Whole Cells ◽  
Engineered Escherichia Coli

Biocatalytic production of (2S,3S)-2,3-butanediol from diacetyl using whole cells of engineered Escherichia coli

2012 ◽  
Vol 115 ◽  
pp. 111-116 ◽  
Author(s):  
Lixiang Li ◽  
Yu Wang ◽  
Lijie Zhang ◽  
Cuiqing Ma ◽  
Ailong Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Whole Cells ◽  
Engineered Escherichia Coli ◽  
Biocatalytic Production

scholarly journals Enzymatic Menthol Production: One-Pot Approach Using Engineered Escherichia coli

2015 ◽  
Vol 4 (10) ◽  
pp. 1112-1123 ◽  
Author(s):  
Helen S. Toogood ◽  
Aisling Ní Cheallaigh ◽  
Shirley Tait ◽  
David J. Mansell ◽  
Adrian Jervis ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
One Pot ◽  
Engineered Escherichia Coli

Biosynthesis of D-danshensu from L-DOPA using engineered Escherichia coli whole cells

2019 ◽  
Vol 103 (15) ◽  
pp. 6097-6105 ◽  
Author(s):  
Tianzhen Xiong ◽  
Jing Jiang ◽  
Yajun Bai ◽  
Tai-ping Fan ◽  
Ye Zhao ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Whole Cells ◽  
Engineered Escherichia Coli

Selective carboxylation of substituted phenols with engineered Escherichia coli whole-cells

2018 ◽  
Vol 59 (42) ◽  
pp. 3810-3815 ◽  
Author(s):  
Chang Peng ◽  
Yuxue Liu ◽  
Xiaojia Guo ◽  
Wujun Liu ◽  
Qing Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Substituted Phenols ◽  
Whole Cells ◽  
Engineered Escherichia Coli
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