Ghrline Promot the Ovarian Cancer Cell Autophagy by LncRNA linc00598

ObjectiveTo explore the autophagy effect of ghrelin on the ovarian cancer cell line SK-OV-3. And the lncRNA which regulate the ghrelin effect SK-OV-3 autophagy was showed.Methodsthe expression of ghrelin in the ovarian cancer tissues was analyzed according GEPIA database and HPA database. The CCK-8 was used to detect the the optimal concentration of ghrelin effect on the SK-OV-3. The influence on the SK-OV-3 cell autophagy by ghrelin was showed by detecting the expression of Beclin-1, LC3Ⅰand LC3Ⅱusing western blot. Linc00598 selected as the effecting the SK-OV-3 cells autophagy by ghrelin using RNA-Seq. And the Linc00598 which was silenced or overexressed promote the SK-OV-3 cells autophagy treated by ghrelin though western blot.ResultsGhrelin was expressed low in the ovarian cancer tissues. Ghrelin concentratio of 600 ng/ml was the optimal concentration o and 24 h was the optimal time. Ghrelin can promote the SK-OV-3 cell autophagy. Ghrelin mainly through linc00598 to promote the SK-OV-3 cells autophagy. When the linc00598 silenced, ghrelin promote SK-OV-3 cells autophagy was inhibited. And When the linc00598 overexpressed, ghrelin promote SK-OV-3 cells autophagy was inhanced.ConclusionsGhrelin promote SK-OV-3 cells autophagy. Additionally, we proved that ghrelin regulated the progression of SK-OV-3 cells autophagy by linc00598/ Beclin1 axis.

Composition and Efficacy of Essential Oil Nanoemulsions

Essential oil, endorsed as a natural cum powerful ingredient, encompasses incredible properties, and various approaches have been proposed and spurred over the last decades. One among them is the nano-emulsification of the essential oils, and the sub-micron size provides prolonged shelf life, ease of formulation and handling, nullification of the stated obstacles, as well as the augmentation of the essential oil antimicrobial activity. This chapter primarily reinforces and is well-articulated on (1) the components and its precise concentration adequate to fabricate nanoemulsion; (2) critical analysis on the experimentally inferred antimicrobial mechanism and the extent of contribution granted by the optimal concentration of the components in the nanoemulsion; (3) scrutinization of the possible impact rendered by the components on the other characteristics of nanoemulsion aside from antimicrobial potency. Altogether, the importance of careful formulation of the essential oil-loaded nanoemulsion is emphasized extensively.

The change of microglia numbers within the mice retina, optic nerve and chiasm following intravitreal AAV2-GFP injection

Purpose To explore the optimized concentration of AAV2-GFP for sparse transfection of retinal ganglion cells (RGCs) and optic nerve (ON), and to examine the changes of microglial morphology and distribution in the retina, optic nerve and chiasm after injection. Methods We defined the optimal concentration of AAV2-GFP for sparse labeling of RGCs and axons in WT mice. We further explored the changes of microglial morphology and distribution in the retina, optic nerve and chiasm after intravitreal injection in CX3CR1+/GFP mice. Results 14 days after intravitreal injection of AAV2-GFP, live imaging of the retina showed that fundus fluorescence was very strong and dense at 2.16 × 1011 VG/retina, 2.16 × 1010 VG/retina, 2.16 × 109 VG/retina. RGCs were sparsely marked at a concentration 1:1000 (2.16 × 108 VG/retina) and fundus fluorescence was weak. The transfected RGCs and axons were unevenly distributed in the retina and significantly more RGCs were transfected near the injection site of AAV2-GFP compared to the other sites of the flat-mounted retina. Microglia density increased significantly in the retina and part of optic nerve, but not in the optic chiasm. The morphology of microglia was largely unchanged. Conclusions AAV2-GFP was highly efficient and the optimal concentration of sparsely labeled RGCs was 1:1000 (2.16 × 108 VG/retina). After intravitreal injection of AAV2-GFP, the number of microglia increased partly. The morphology of microglia was comparable.

Flexible, scalable, and efficient thermoelectric touch detector based on PDMS and Graphite flakes

This paper presents freestanding thermoelectric touch detectors consisting of graphite conductive flakes into a polydimethylsiloxane matrix. An optimal concentration of graphite flakes (45 wt%) lead to robust and homogeneous detectors that exhibited signal-noise ratio values up to 170 with rise and falling times below 1 s and 7 s, respectively. The detectors performance was stable over continuous operation and did not reveal significant degradation while bended under different curvature radii (45, 25 and 15 mm) and consecutive bending cycles. Moreover, the twist of the thermal gradient direction between the electrodes of the detector enables a Yes or No response which opens new usage possibilities. Therefore, this work provides an efficient way to develop robust, low-cost, and scalable thermal detectors with potential use in wearable technologies.

OPTIMAL CONCENTRATION OF BASIL LEAF EXTRACT (Ocimum basilicum L.) IN INHIBITING THE GROWTH OF Streptococcus mutans IN VITRO

Introduction: One of flora in the oral cavity is Streptococcus mutans as a cause of dental caries. Various ways can be done to suppress its growth, one of them by using mouthwash which is used contains a lot of chemicals, so an alternative is needed by using herbal ingredients, including basil (Ocimum basilicum L.) leaves that contain essential oils, methyl eugenol, phenols, and flavonoids which are able to work as antibacterial. The purpose of this study was to determine the inhibition and optimal concentration of basil leaf extract on the growth of Streptococcus mutans. Materials and method: The method used agar Kirby Bauer method with seven treatments of leaf extract with concentrations of 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, and methanol solution as control group. The culture medium used was Muller Hinton Blood Agar. Results and discussions: The Kruskall Wallis test showed a significant difference between treatment groups. Mann Whitney U-Test test, found that the control group, basil leaf extract concentrations of 1.5%, 2%, 2.5% and 3% were tested with concentrations of 3.5% and 4% had a significant difference. The test between groups of 3.5% and 4% basil leaf extract did not show a significant difference. Conclusion: this study was that in vitro basil leaf extract with a concentration of 3.5% and 4% had inhibitory power on the growth of Streptococcus mutans and basil leaf extract with a concentration of 3.5% optimally inhibited the growth of Streptococcus mutans in vitro

Study of the influence of preparation parameters on sorption properties of porous collagen material

In this work, the dependences of the sorption properties of the porous polymeric materials prepared from the cross- linked collagen on their composition and process specifications were studied. The optimal concentration of glutaraldehyde as a cross-linking agent was 0.5 wt %, the mass ratio of fish and leather waste was 1:1, and the drying temperature was 25 °C. Sorbent samples prepared using optimal parameters were effective in the sorption of petroleum products.

ASSESSMENT OF THE QUALITY OF THE ASSET POOL FORMED FOR SECURITIZATION IN THE CONTEXT OF ANALYTICAL PROVISION OF THE MANAGEMENT SYSTEM

The lack of an effective methodology for the analysis of asset securitization operations causes problems in the formation of a strategy for managing the process of financing the activities of the initiator of the securitization. The article substantiates the areas of analytical support of the management system in terms of asset securitization: the formation of financial and economic justification of the feasibility and feasibility of securitization of assets (study of potential investors and analysis of prospects for placement of securities; diligence); assessment of the original assets of the originator for the possibility of their securitization; identification of the impact of securitization of assets on the financial performance of the originator; assessment of risks associated with the use of securitization of assets); analysis of the quality of the asset pool generated by the originator for securitization; evaluation of the effectiveness of securitization operations for its initiator. It is also proposed to analyze the quality of the pool of assets generated by the originator for securitization, in the following sequence: assessment of the probability of default and the amount of expected losses on the pool of assets to be securitized; identification of factors influencing the early repayment of pool assets; assessment of the parameters of the formed pool of securitized assets, based on the analysis of its structure and the selected system of indicators; modeling of future payment flows in the pool of securitized assets, which provides the formation of analytical information to decide on the possibility of securitization using the assets available in the originator and diversification of risks through optimal concentration of assets.

Formamide deionized accelerates the somatic embryogenesis of Cunninghamia lanceolata

Aim of the study: To improve the efficiency of the somatic embryogenesis (SE) in Cunninghamia lanceolata. Area of the study: The study was conducted at Nanjing Forestry University (Nanjing, China). Material and methods: Immature cones of C. lanceolata, genotype 01A1 which was planted in Yangkou State-owned Forest Farm (Fujian, China), were used to induced callus. These calli were used to induce SE, concentration gradients of 0 g/L, 0.01134 g/L, 0.1134 g/L, 1.1134 g/L and 11.34 g/L of FD was added, to explore the optimal concentration for promoting SE of C. lanceolata. Main results: Low concentration of FD promoted the maturation of somatic embryos, while high concentration of FD lead to browning of embryogenic callus. The seedling rate and rooting number of seedlings induced by different concentrations of FD were significantly different. Research highlights: This study may aid in the rapid maturation of C. lanceolata somatic embryos and is useful for accelerated C. lanceolata breeding.

thorium(IV), sorbent, sorption, desorption, clay

The article considers the danger of thorium due to its radioactive toxicity. There has been proved the diversity of thorium compounds and the influence of thorium on a human body and the environment. A polymer chelating sorbent containing fragments of 4-amino - thiouracil has been investigated. An isotherm of thorium sorption with the sorbent is constructed and the optimal concentration conditions are determined: pH = 4; = 6 • 10–3 mol/l; Vob = 25 ml; msorb = 0.03 g; sorptive capacity (CE) = 1 163 mg/g. The degree of extraction of thorium(IV) ions under optimal conditions exceeds 95%. The developed technique was applied to extract thorium from clay. Studying sorption was carried out under static conditions using special equipment.

A Selective Medium for the Recovery and Enumeration of Fomitopsis meliae, Causing Lemon Canker and Wood Rot

A selective medium (FMS medium) was developed for the isolation and quantification of Fomitopsis meliae, the causal agent of lemon canker and brown wood rot, from plants, soil, and air. The optimal concentration and combination of fungicides and antibiotics was evaluated to determine the most selective condition for growing F. meliae. The resultant composition of the medium (FMS) per litre (pH 3.5) was: 16 mg thiophanate-methyl, 8 mg dichloran, 5 mg 2-phenylphenol, 100 mg fluopyram, 0.5 mg fludioxonil, 100 mg chloramphenicol, 100 mg streptomycin, 15 g malt extract, 2.5 g mycological peptone, and 15 g agar. The fungus was successfully isolated and enumerated from air, soil and plant tissues using FMS medium. Furthermore, FMS medium almost completely inhibited the growth of other plant pathogenic fungi, soil and air saprophytes. This selectivity was high enough to estimate spore inoculum of F. meliae in an air sample or as a spore trapping device in commercial lemon orchards. FMS medium will be useful for studying epidemiology and management of F. meliae.