scholarly journals Development and validation of an LC-MS/MS method with a broad linear dynamic range for the quantification of tivozanib in human and mouse plasma, mouse tissue homogenates, and culture medium

2019 ◽  
Vol 1125 ◽  
pp. 121723
Author(s):  
M.A.C. Bruin ◽  
H. Rosing ◽  
L. Lucas ◽  
J. Wang ◽  
A.D.R. Huitema ◽  
...  
Keyword(s):  
Culture Medium ◽  
Dynamic Range ◽  
Mouse Tissue ◽  
Linear Dynamic Range ◽  
Mouse Plasma ◽  
Linear Dynamic ◽  
Tissue Homogenates ◽  
Development And Validation ◽  
Human And Mouse

Development and Validation of a Rapid LC-MS/MS Method for Simultaneous Determination of Kaempferol and Quercetin in Thespesia populnea Extract

2017 ◽  
Vol 100 (4) ◽  
pp. 971-975 ◽  
Author(s):  
Hiteksha Panchal ◽  
Mamta B Shah
Keyword(s):  
Simultaneous Determination ◽  
Dynamic Range ◽  
Herbal Extract ◽  
Linear Dynamic Range ◽  
Linear Dynamic ◽  
Thespesia Populnea ◽  
Accuracy And Precision ◽  
Development And Validation ◽  
Interday Precision

Abstract In this study, a simple and rapid LC with tandem MS method was developed and validated for the simultaneous determination of kaempferol and quercetin in Thespesia populnea extract. The compounds were eluted using a Gemini C18 column (50 × 2.0 mm, 3 μm), with the mobile phase consisting of acetonitrile–0.3% formic acid in water at the flow rate of 0.400 mL/min. The assay exhibited a linear dynamic range of 25–2500 ng/mL for both kaempferol and quercetin. The values for intra- and interday precision and accuracy were well within the generally accepted criteria for analytical methods (<15%). Selectivity, linearity, LOD, LOQ, accuracy, and precision were evaluated for both analytes. The proposed method is accurate and sensitive and can be used for the routine quantification of kaempferol and quercetin in the herbal extract and in polyherbal formulations.

Convenient Fiber-Optic-Based Sample Cell for Shpol'skii and Low-Temperature Phosphorescence Spectrometry

1989 ◽  
Vol 43 (3) ◽  
pp. 422-425 ◽  
Author(s):  
Richard T. Madison ◽  
Mary K. Carroll ◽  
Gary M. Hieftje
Keyword(s):  
Low Temperature ◽  
Fiber Optic ◽  
Dynamic Range ◽  
Detection Limits ◽  
Linear Dynamic Range ◽  
Sample Cell ◽  
Linear Dynamic ◽  
Light Guides

A sample cell for observing the Shpol'skii effect at 77 K is described and analytically assessed. The cell employs fiber-optic light guides to transport excitation and emission radiation. The system is compact, inexpensive, and simple to construct from commercially available laboratory components, and it alleviates several problems inherent in conventional refrigerated-cell designs. Detection limits for anthracene, coronene, and pyrene obtained with the sample cell are 8.8 × 10−8 M, 8.4 × 10−7 M, and 3.5 × 10−7 M, respectively. The linear dynamic range for each compound is 2 to 3 orders of magnitude.

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