Development and validation of ultra-performance liquid chromatography/tandem mass spectrometric methods for simultaneous and rapid determination of contezolid and its major metabolite M2 in plasma and urine samples and its application to a study in subjects with moderate liver impairment

2022 ◽  
pp. 123129
Author(s):  
Yu Wang ◽  
Hailan Wu ◽  
Junzhen Wu ◽  
Yaxin Fan ◽  
Xiaofen Liu ◽  
...  
Keyword(s):  
Rapid Determination ◽  
Ultra Performance Liquid Chromatography ◽  
Mass Spectrometric ◽  
Tandem Mass ◽  
Liver Impairment ◽  
Liquid Chromatography Tandem Mass ◽  
Plasma And Urine Samples ◽  
Tandem Mass Spectrometric ◽  
Development And Validation

scholarly journals Rapid Determination of Isomeric Benzoylpaeoniflorin and Benzoylalbiflorin in Rat Plasma by LC-MS/MS Method

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Chuanqi Zhou ◽  
Xiaoke Wang
Keyword(s):  
Linear Response ◽  
Therapeutic Agent ◽  
Rapid Determination ◽  
Rat Plasma ◽  
Mass Spectrometric ◽  
Tandem Mass ◽  
Liquid Chromatography Tandem Mass ◽  
Bioanalytical Application ◽  
Tandem Mass Spectrometric

Benzoylpaeoniflorin (BP) is a potential therapeutic agent against oxidative stress related Alzheimer’s disease. In this study, a more rapid, selective, and sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed to determine BP in rat plasma distinguishing with a monoterpene isomer, benzoylalbiflorin (BA). The method showed a linear response from 1 to 1000 ng/mL (r>0.9950). The precision of the interday and intraday ranged from 2.03 to 12.48% and the accuracy values ranged from −8.00 to 10.33%. Each running of the method could be finished in 4 minutes. The LC-MS/MS method was validated for specificity, linearity, precision, accuracy, recovery, and stability and was found to be acceptable for bioanalytical application. Finally, this fully validated method was successfully applied to a pharmacokinetic study in rats following oral administration.

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