Development of Level A in vitro-in vivo correlations for peptide loaded PLGA microspheres

Repair of articular cartilage defects is a challenging aspect of clinical treatment. Kartogenin (KGN), a small molecular compound, can induce the differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) into chondrocytes. Here, we constructed a scaffold based on chondrocyte extracellular matrix (CECM) and poly(lactic-co-glycolic acid) (PLGA) microspheres (MP), which can slowly release KGN, thus enhancing its efficiency. Cell adhesion, live/dead staining, and CCK-8 results indicated that the PLGA(KGN)/CECM scaffold exhibited good biocompatibility. Histological staining and quantitative analysis demonstrated the ability of the PLGA(KGN)/CECM composite scaffold to promote the differentiation of BMSCs. Macroscopic observations, histological tests, and specific marker analysis showed that the regenerated tissues possessed characteristics similar to those of normal hyaline cartilage in a rabbit model. Use of the PLGA(KGN)/CECM scaffold may mimic the regenerative microenvironment, thereby promoting chondrogenic differentiation of BMSCs in vitro and in vivo. Therefore, this innovative composite scaffold may represent a promising approach for acellular cartilage tissue engineering.

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Sophoridine-loaded PLGA microspheres for lung targeting: preparation, in vitro, and in vivo evaluation

Drug Delivery ◽

10.1080/10717544.2016.1223210 ◽

2016 ◽

Vol 23 (9) ◽

pp. 3674-3680 ◽

Cited By ~ 7

Author(s):

Wenping Wang ◽

Yaqin Cai ◽

Guangxing Zhang ◽

Yanhua Liu ◽

Hong Sui ◽

...

Keyword(s):

Plga Microspheres ◽

In Vivo Evaluation ◽

Lung Targeting

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Synchronic release of two hormonal contraceptives for about one month from the PLGA microspheres: In vitro and in vivo studies

Journal of Controlled Release ◽

10.1016/j.jconrel.2008.04.022 ◽

2008 ◽

Vol 129 (3) ◽

pp. 192-199 ◽

Cited By ~ 38

Author(s):

Yi Sun ◽

JianCheng Wang ◽

Xuan Zhang ◽

ZhiJun Zhang ◽

Yan Zheng ◽

...

Keyword(s):

In Vivo Studies ◽

Hormonal Contraceptives ◽

Plga Microspheres

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In Vitro-In Vivo Relationship of Amorphous Insoluble API (Progesterone) in PLGA Microspheres

Pharmaceutical Research ◽

10.1007/s11095-017-2258-4 ◽

2017 ◽

Vol 34 (12) ◽

pp. 2787-2797 ◽

Cited By ~ 6

Author(s):

Chenguang Pu ◽

Qiao Wang ◽

Hongjuan Zhang ◽

Jingxin Gou ◽

Yuting Guo ◽

...

Keyword(s):

Plga Microspheres ◽

Relationship Of

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Development of Risperidone PLGA Microspheres

Journal of Drug Delivery ◽

10.1155/2014/620464 ◽

2014 ◽

Vol 2014 ◽

pp. 1-11 ◽

Cited By ~ 21

Author(s):

Susan D’Souza ◽

Jabar A. Faraj ◽

Stefano Giovagnoli ◽

Patrick P. DeLuca

Keyword(s):

Steady State ◽

Sustained Release ◽

Sustained Delivery ◽

Plga Microspheres ◽

Sprague Dawley ◽

Multiple Dosing ◽

Duration Of Action ◽

Sprague Dawley Rats

The aim of this study was to design and evaluate biodegradable PLGA microspheres for sustained delivery of Risperidone, with an eventual goal of avoiding combination therapy for the treatment of schizophrenia. Two PLGA copolymers (50 : 50 and 75 : 25) were used to prepare four microsphere formulations of Risperidone. The microspheres were characterized by several in vitro techniques. In vivo studies in male Sprague-Dawley rats at 20 and 40 mg/kg doses revealed that all formulations exhibited an initial burst followed by sustained release of the active moiety. Additionally, formulations prepared with 50 : 50 PLGA had a shorter duration of action and lower cumulative AUC levels than the 75 : 25 PLGA microspheres. A simulation of multiple dosing at weekly or 15-day regimen revealed pulsatile behavior for all formulations with steady state being achieved by the second dose. Overall, the clinical use of Formulations A, B, C, or D will eliminate the need for combination oral therapy and reduce time to achieve steady state, with a smaller washout period upon cessation of therapy. Results of this study prove the suitability of using PLGA copolymers of varying composition and molecular weight to develop sustained release formulations that can tailor in vivo behavior and enhance pharmacological effectiveness of the drug.

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