scholarly journals Misidentification of Mycobacterium smegmatis as Mycobacterium fortuitum by DNA line probe assay

2021 ◽  
Vol 25 ◽  
pp. 100268
Author(s):  
Madiha Fida ◽  
Elena Beam ◽  
Nancy L. Wengenack
Keyword(s):  
Mycobacterium Smegmatis ◽  
Mycobacterium Fortuitum ◽  
Line Probe Assay ◽  
Probe Assay

INNO-LiPA DNA line probe assay misidentification of M. smegmatis as Mycobacterium fortuitum complex

2019 ◽  
Vol 95 (3) ◽  
pp. 114858 ◽  
Author(s):  
Theo van den Broek ◽  
Nard G. Janssen ◽  
David J. Hetem ◽  
Wouter Bekers ◽  
Miranda Kamst ◽  
...  
Keyword(s):  
Mycobacterium Fortuitum ◽  
Line Probe Assay ◽  
Probe Assay

Analysis of rpoB Gene in Rifampin-Resistant M. Tuberculosis by Direct Sequencing and Line Probe Assay

1997 ◽  
Vol 44 (2) ◽  
pp. 251
Author(s):  
Min Ki Lee ◽  
Yun Seong Kim ◽  
Hyo Jin Lee ◽  
Du Su Cheon ◽  
Sang Myung Yun ◽  
...  
Keyword(s):  
Direct Sequencing ◽  
Rpob Gene ◽  
Line Probe Assay ◽  
Probe Assay

Tubercular Abscess of the Spleen in a Child: Conservative Management and Long-Term Follow-Up

2017 ◽  
Vol 13 (04) ◽  
pp. 316-318
Author(s):  
Vijai Williams ◽  
Sarvanan Chandrasekaran ◽  
Keerthivasan Seetharaman ◽  
Kushaljit Sodhi ◽  
Devi Dayal
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Splenic Abscess ◽  
Complete Healing ◽  
Rare Occurrence ◽  
Line Probe Assay ◽  
Rare Manifestation ◽  
Long Term Follow Up ◽  
Probe Assay

AbstractIsolated involvement of the spleen is a rare manifestation of childhood tuberculosis. The rare occurrence and presence of nonspecific clinical features often pose diagnostic challenges even in countries where tuberculosis is endemic. Splenectomy is often performed for establishing the diagnosis or achieving a cure in combination with antitubercular therapy (ATT). In this report, we describe a boy who presented with nonspecific symptoms and was detected to have a well-defined lesion in the spleen on ultrasonography. The diagnosis of tubercular splenic abscess was made after line probe assay for the detection of Mycobacterium tuberculosis and cytomorphology of the aspirated material. The tubercular abscess showed complete healing after 9 months of four-drug ATT.

scholarly journals Comparison of DNA Enzyme Immunoassay and Line Probe Assays (Inno-LiPA HCV I and II) for Hepatitis C Virus Genotyping

1998 ◽  
Vol 36 (5) ◽  
pp. 1461-1463 ◽  
Author(s):  
S. Le Pogam ◽  
F. Dubois ◽  
R. Christen ◽  
C. Raby ◽  
A. Cavicchini ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Enzyme Immunoassay ◽  
Untranslated Regions ◽  
Line Probe Assay ◽  
The Core ◽  
Probe Assay ◽  
Dna Enzyme Immunoassay

Two methods for genotyping hepatitis C virus (DNA enzyme immunoassay [DEIA] and line probe assay [Inno-LiPA HCV I and II]) were compared on 120 samples and of these 87% were assigned to the same subtype by both assays. There were 15 subtyping discrepancies which involved 5% of type 1 isolates and 90% of type 2 isolates. Amplified products from the core and 5′ untranslated regions (UTR) were sequenced to resolve conflicts. Type 1 discordant samples had a guanosine at position −99 in the 5′ UTR, a characteristic of genotype 1b, and a core region typical of subtype 1a. The eight isolates classified as 2a/2c by LiPA and as subtype 2c by DEIA belonged to type 2.

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