Evaluation of the line probe assay (LiPA) for rapid detection of rifampicin resistance in Mycobacterium tuberculosis

Tuberculosis ◽  
2004 ◽  
Vol 84 (5) ◽  
pp. 311-316 ◽  
Author(s):  
Pontus Juréen ◽  
Jim Werngren ◽  
Sven E Hoffner
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Rapid Detection ◽  
Rifampicin Resistance ◽  
Line Probe Assay ◽  
Probe Assay

AID TB resistance line probe assay for rapid detection of resistant Mycobacterium tuberculosis in clinical samples

2015 ◽  
Vol 70 (4) ◽  
pp. 400-408 ◽  
Author(s):  
B. Molina-Moya ◽  
A. Lacoma ◽  
C. Prat ◽  
J. Diaz ◽  
A. Dudnyk ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Rapid Detection ◽  
Clinical Samples ◽  
Line Probe Assay ◽  
Probe Assay

scholarly journals Evaluation of a line probe assay for the rapid detection of gyrA mutations associated with fluoroquinolone resistance in multidrug-resistant Mycobacterium tuberculosis

2011 ◽  
Vol 60 (2) ◽  
pp. 184-188 ◽  
Author(s):  
Hiroki Ando ◽  
Satoshi Mitarai ◽  
Yuji Kondo ◽  
Toshinori Suetake ◽  
Seiya Kato ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Global Health ◽  
Dna Sequencing ◽  
Rapid Detection ◽  
Multidrug Resistant ◽  
Surveillance Study ◽  
Fluoroquinolone Resistance ◽  
National Surveillance ◽  
Line Probe Assay ◽  
Probe Assay

The aim of this study was to establish the importance of detecting fluoroquinolone (FQ) resistance in multidrug resistant (MDR) Mycobacterium tuberculosis, and to show the usefulness of a hybridization-based line probe assay (LiPA) for detecting gyrA mutations. Thirty-three MDR M. tuberculosis isolates were collected from a total of sixty MDR isolates identified in Japan over 6 months during a national surveillance study in 2002. Seventeen MDR isolates were collected by the National Center for Global Health and Medicine in Japan over 6 years from 2003 to 2008. These 50 isolates were examined for FQ susceptibility, and analysed by LiPA and gyrA sequencing. Among them, 22 (44 %) showed FQ resistance. All FQ-resistant isolates had at least one mutation in gyrA. The results of the LiPA were fully consistent with the DNA sequencing results. Given that on the basis of our results almost half of the MDR M. tuberculosis isolates in Japan might have resistance to FQ, it is important to monitor FQ resistance in patients with MDR tuberculosis (TB), as well as with drug-susceptible TB, prior to commencing treatment. For the detection of FQ resistance, LiPA is useful and can rapidly and efficiently assess FQ resistance.

Assessment of line probe assay for rapid detection of extensively drug-resistant Mycobacterium tuberculosis isolates

2021 ◽  
Vol 9 (5) ◽  
pp. 65
Author(s):  
Jalil Kardan-Yamchi ◽  
Hossein Kazemian ◽  
HamidReza Abtahi ◽  
Sirus Amini ◽  
MohammadMehdi Feizabadi
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Rapid Detection ◽  
Drug Resistant ◽  
Line Probe Assay ◽  
Extensively Drug Resistant ◽  
Probe Assay

Multi-center evaluation of GenoType MTBDRsl line probe assay for rapid detection of pre-XDR and XDR Mycobacterium tuberculosis in China

2018 ◽  
Vol 77 (4) ◽  
pp. 328-334 ◽  
Author(s):  
Yazhou Gao ◽  
Zhengdong Zhang ◽  
Jianping Deng ◽  
Mikael Mansjö ◽  
Zhu Ning ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Rapid Detection ◽  
Line Probe Assay ◽  
Probe Assay

scholarly journals Mutations in the rpoB Gene of Rifampin-Resistant Mycobacterium tuberculosis Strains Isolated Mostly in Asian Countries and Their Rapid Detection by Line Probe Assay

1999 ◽  
Vol 37 (8) ◽  
pp. 2663-2666 ◽  
Author(s):  
Kazue Hirano ◽  
Chiyoji Abe ◽  
Mitsuyoshi Takahashi
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Rapid Detection ◽  
Rpob Gene ◽  
Line Probe Assay ◽  
Asian Countries ◽  
Nucleotide Substitutions ◽  
In Vitro Susceptibility ◽  
Nucleotide Insertion ◽  
Probe Assay

Mutations in the rpoB gene of 90 rifampin-resistantMycobacterium tuberculosis isolates mostly from Asian countries were analyzed. Ten distinct single-nucleotide substitutions were found among the isolates by automated sequencing. A 3-nucleotide insertion was found in two isolates, and no mutation was found in five isolates (5.6%). A reverse hybridization-based line probe assay (INNO-LiPA Rif TB) for rapid detection of the mutations was evaluated with these isolates. Concordance rates with sequencing results for five wild-type probes (S probes) and four probes for specific mutations (R probes) were 96.7 and 100%, respectively. The overall concordance rate with the in vitro susceptibility testing results was 92.2% (83 of 90 isolates). These results indicate that a commercial line probe assay kit may be useful for rapid diagnosis of rifampin-resistant tuberculosis.

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