Three-dimensional culture and characterization of mononuclear cells from human bone marrow

Cytotherapy ◽  
2015 ◽  
Vol 17 (4) ◽  
pp. 458-472 ◽  
Author(s):  
Jennifer J. Bara ◽  
Marietta Herrmann ◽  
Ursula Menzel ◽  
Lorin Benneker ◽  
Mauro Alini ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mononuclear Cells ◽  
Three Dimensional ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Three Dimensional Culture

Phenotypic Characterization of Distinct Human Bone Marrow-Derived MSC Subsets

2009 ◽  
Vol 1176 (1) ◽  
pp. 124-134 ◽  
Author(s):  
Hans-Jörg Bühring ◽  
Sabrina Treml ◽  
Flavianna Cerabona ◽  
Peter De Zwart ◽  
Lothar Kanz ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Phenotypic Characterization

scholarly journals Three-Dimensional Arrangement of Human Bone Marrow Microvessels Revealed by Immunohistology in Undecalcified Sections

PLoS ONE ◽  
2016 ◽  
Vol 11 (12) ◽  
pp. e0168173 ◽  
Author(s):  
Birte S. Steiniger ◽  
Vitus Stachniss ◽  
Verena Wilhelmi ◽  
Anja Seiler ◽  
Katrin Lampp ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Three Dimensional ◽  
Human Bone ◽  
Human Bone Marrow

Preparation and Characterization of Human Bone Marrow-Derived Macrophages

1985 ◽  
Vol 38 (4) ◽  
pp. 541-552 ◽  
Author(s):  
David A. Hume ◽  
William Allan ◽  
Jeffrey Golder ◽  
Ross W. Stephens ◽  
William F. Doe ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Human Bone ◽  
Human Bone Marrow

Isolation Yield and Osteogenic Potential of Human Bone Marrow Stromal Cells are Maintained Irrespective of Age or Gender

2007 ◽  
Vol 361-363 ◽  
pp. 1149-1152
Author(s):  
Jeong Joon Yoo ◽  
Jeon Hyun Bang ◽  
Kyung Hoi Koo ◽  
Kang Sup Yoon ◽  
Hee Joong Kim
Keyword(s):  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Mononuclear Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Osteogenic Potential ◽  
Donor Age

The relationships between donor age and gender and initial isolation yield and the osteogenic potentials of human bone marrow stromal cells (hBMSCs) have not been clearly elucidated. The authors investigated whether isolation yields and the osteogenic differentiation potentials of hBMSCs are indeed dependent on donor age or gender. Fresh bone marrow was aspirated from iliac crest of 72 donors (mean age 54.1 years; range, 23-84 years; 39 men and 33 women) undergoing total hip arthroplasty. Numbers of mononuclear cells, numbers of colony forming unit-fibroblasts (CFU-Fs) and alkaline phosphatase (ALP)-positive CFU-Fs, and numbers of BMSCs after isolation culture were not found to be significantly dependent on donor age or gender. Moreover, no significant age- or gender-related differences were observed in terms of the proliferation activities, ALP activities, and calcium contents of BMSCs during in vitro osteogenic differentiation. The data obtained from 72 human donors revealed no significant age- or genderrelated differences among hBMSCs in terms of isolation yields, proliferation activities, and osteogenic potentials.

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