scholarly journals Dissolved organic phosphorus uptake by marine phytoplankton is enhanced by the presence of dissolved organic nitrogen

2020 ◽  
Vol 530-531 ◽  
pp. 151434
Author(s):  
Mark F. Fitzsimons ◽  
Ian Probert ◽  
Fanny Gaillard ◽  
Andrew P. Rees
Keyword(s):  
Dissolved Organic Nitrogen ◽  
Organic Nitrogen ◽  
Organic Phosphorus ◽  
Phosphorus Uptake ◽  
Marine Phytoplankton ◽  
Dissolved Organic Phosphorus

Seasonal Changes in the Organic Forms of Carbon, Nitrogen and Phosphorus in Sea Water at E1 in the English Channel During 1968

1973 ◽  
Vol 53 (3) ◽  
pp. 695-703 ◽  
Author(s):  
M. W. Banoub ◽  
P. J. leB. Williams
Keyword(s):  
Organic Carbon ◽  
Dissolved Organic Carbon ◽  
Particulate Organic Carbon ◽  
Dissolved Organic Nitrogen ◽  
Organic Nitrogen ◽  
Sea Water ◽  
Organic Phosphorus ◽  
English Channel ◽  
Dissolved Organic Phosphorus ◽  
Nitrogen And Phosphorus

A seasonal survey of particulate and dissolved organic material was made at E1 in the English Channel during 1968. The average integral mean values were: dissolved organic carbon 65 μg-at C/l (780 μg C/l); dissolved organic nitrogen 4·6 μg-at N/l (64 μg N/l); dissolved organic phosphorus 0·12 μg-at P/l (3·8 μg P/l); particulate organic carbon 190 μg C/l and particulate organic nitrogen 21 μg N/l. Dissolved organic carbon and the particulate organic carbon and nitrogen showed increases subsequent to the spring bloom; such increases were less evident in the dissolved organic nitrogen results and not apparent in those of dissolved organic phosphorus.Analyses were also made in March and June in 1969 at two other stations in the English Channel, in addition to E1. The results from the three stations were basically similar.

scholarly journals Non-Conventional Metal Ion Cofactor Requirement of Dinoflagellate Alkaline Phosphatase and Translational Regulation by Phosphorus Limitation

2019 ◽  
Vol 7 (8) ◽  
pp. 232 ◽  
Author(s):  
Xin Lin ◽  
Chentao Guo ◽  
Ling Li ◽  
Tangcheng Li ◽  
Senjie Lin
Keyword(s):  
Alkaline Phosphatase ◽  
Metal Ion ◽  
Translational Regulation ◽  
Organic Phosphorus ◽  
Niche Differentiation ◽  
Phosphorus Limitation ◽  
Marine Phytoplankton ◽  
Phosphate Limitation ◽  
Dissolved Organic Phosphorus ◽  
Metal Cofactor

Alkaline phosphatase (AP) enables marine phytoplankton to utilize dissolved organic phosphorus (DOP) when dissolved inorganic phosphate (DIP) is depleted in the ocean. Dinoflagellate AP (Dino-AP) represents a newly classified atypical type of AP, PhoAaty. Despite While being a conventional AP, PhoAEC is known to recruit Zn2+ and Mg2+ in the active center, and the cofactors required by PhoAaty have been contended and remain unclear. In this study, we investigated the metal ion requirement of AP in five dinoflagellate species. After AP activity was eliminated by using EDTA to chelate metal ions, the enzymatic activity could be recovered by the supplementation of Ca2+, Mg2+ and Mn2+ in all cases but not by that of Zn2+. Furthermore, the same analysis conducted on the purified recombinant ACAAP (AP of Amphidinium carterae) verified that the enzyme could be activated by Ca2+, Mg2+, and Mn2+ but not Zn2+. We further developed an antiserum against ACAAP, and a western blot analysis using this antibody showed a remarkable up-regulation of ACAAP under a phosphate limitation, consistent with elevated AP activity. The unconventional metal cofactor requirement of Dino-AP may be an adaptation to trace metal limitations in the ocean, which warrants further research to understand the niche differentiation between dinoflagellates and other phytoplankton that use Zn–Mg AP in utilizing DOP.

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