Efficiency of selected wastewater treatment processes in removing estrogen compounds and reducing estrogenic activity using the T47D-KBLUC reporter gene assay

2020 ◽  
Vol 260 ◽  
pp. 110135 ◽  
Author(s):  
Muyasu Grace Kibambe ◽  
Maggie N.B. Momba ◽  
A.P. Daso ◽  
M.C. Van Zijl ◽  
Marthie A.A. Coetzee
Keyword(s):  
Wastewater Treatment ◽  
Reporter Gene ◽  
Estrogenic Activity ◽  
Reporter Gene Assay ◽  
Treatment Processes ◽  
Gene Assay

Evaluation of Estrogenic Activity of Wastewater: Comparison Among In Vitro ERα Reporter Gene Assay, In Vivo Vitellogenin Induction, and Chemical Analysis

2015 ◽  
Vol 49 (10) ◽  
pp. 6319-6326 ◽  
Author(s):  
Masaru Ihara ◽  
Tomokazu Kitamura ◽  
Vimal Kumar ◽  
Chang-Beom Park ◽  
Mariko O. Ihara ◽  
...  
Keyword(s):  
Chemical Analysis ◽  
Reporter Gene ◽  
Estrogenic Activity ◽  
Reporter Gene Assay ◽  
Gene Assay

In vitro Estrogenicity of Resin Composites

2004 ◽  
Vol 83 (3) ◽  
pp. 222-226 ◽  
Author(s):  
H. Wada ◽  
H. Tarumi ◽  
S. Imazato ◽  
M. Narimatsu ◽  
S. Ebisu
Keyword(s):  
Bisphenol A ◽  
Reporter Gene ◽  
Estrogenic Activity ◽  
Reporter Gene Assay ◽  
Resin Composites ◽  
Minimum Concentration ◽  
Tert Butyl ◽  
Gene Assay

Previously, we have reported that sealants incorporating bisphenol A dimethacrylate showed estrogenicity by a reporter gene assay. This study tested the hypothesis that commercial composites, which contain various monomers and additives, exhibit estrogenic activity in vitro. The estrogenic activities of eluates obtained from 24 composites and 18 chemicals identified from the composites tested were examined with the use of the reporter gene assay. Among the 24 composites, 6 products were estrogenic, and among the 18 constituents, 1 photostabilizer, 2-hydroxy-4-methoxy-benzophenone (HMBP), 1 photoinitiator, 2,2-dimethoxy-2-phenyl-acetophenone (DMPA), and 1 inhibitor, 2,6-di- tert-butyl- p-cresol (BHT) had significant estrogenic activity. The concentration of HMBP in 4 estrogenic eluates was greater than the minimum concentration required for estrogenicity, and DMPA was found at a higher level than the minimum estrogenic concentration in the remaining 2 estrogenic specimens. These results suggest that the observed estrogenic activity of 6 composites is associated with the elution of either HMBP or DMPA.

Assessment of Polystyrene Extract for Estrogenic Activity in the Rat Uterotrophic Model and an in Vitro Recombinant Receptor Reporter Gene Assay

1998 ◽  
Vol 21 (sup1) ◽  
pp. 101-121 ◽  
Author(s):  
Patricia A. Fail ◽  
John W. Hines ◽  
Tim Zacharewski ◽  
Zhi Fen Wu ◽  
Lester Borodinsky
Keyword(s):  
Reporter Gene ◽  
Estrogenic Activity ◽  
Reporter Gene Assay ◽  
Recombinant Receptor ◽  
Gene Assay

scholarly journals Natural therapies from Costa Rica for the management of menopause: Estrogenic activity in the in vitro ER‐CALUX reporter gene assay

2007 ◽  
Vol 21 (5) ◽  
Author(s):  
Brian Joseph Doyle ◽  
Tracie D. Locklear ◽  
Alice Perez ◽  
Juan Carlos Brenes ◽  
Jorge Laurito‐Gomez ◽  
...  
Keyword(s):  
Costa Rica ◽  
Reporter Gene ◽  
Estrogenic Activity ◽  
Reporter Gene Assay ◽  
Gene Assay

Estrogenic activity of UV filters determined by an in vitro reporter gene assay and an in vivo transgenic zebrafish assay

2002 ◽  
Vol 76 (5-6) ◽  
pp. 257-261 ◽  
Author(s):  
Richard Schreurs ◽  
Peter Lanser ◽  
Willem Seinen ◽  
Bart van der Burg
Keyword(s):  
Reporter Gene ◽  
Estrogenic Activity ◽  
Reporter Gene Assay ◽  
Transgenic Zebrafish ◽  
Uv Filters ◽  
Gene Assay

Biological measurement of estrogenic activity in urine and bile conjugates with the in vitro ER-CALUX reporter gene assay

2002 ◽  
Vol 21 (3) ◽  
pp. 473-479 ◽  
Author(s):  
Juliette Legler ◽  
Arjen Jonas ◽  
Joost Lahr ◽  
A. Dick Vethaak ◽  
Abraham Brouwer ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Estrogenic Activity ◽  
Reporter Gene Assay ◽  
Gene Assay ◽  
Biological Measurement

An improved estrogenic activity reporter gene assay (T47D-KBluc) for detecting estrogenic activity in wastewater and drinking water

2015 ◽  
Vol 98 (3-4) ◽  
pp. 376-384 ◽  
Author(s):  
Hui-Ming Chou ◽  
How-Ran Chao ◽  
Chieh Lin ◽  
Pen-Chi Chiang ◽  
Gen-Shuh Wang ◽  
...  
Keyword(s):  
Drinking Water ◽  
Reporter Gene ◽  
Estrogenic Activity ◽  
Reporter Gene Assay ◽  
Gene Assay
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