Distribution and Estrogenic Risk of Alkylphenolic Compounds, Hormones and Drugs Contained in Water and Natural Surface Sediments, Morelos, Mexico

This study evaluated the distribution and potential estrogenic risk of the presence of bisphenol A (BPA), 4-nonylphenol (4NP), naproxen (NPX), ibuprofen (IBU), 17-β-estradiol (E2) and 17-α-ethinylestradiol (EE2) in water and sediments of the Apatlaco river micro-basin (Morelos, Mexico). The concentration of the determined compounds ranged between <LOD to 86.40 ng·L−1 and <LOD to 3.97 ng g−1 in water and sediments, respectively. The Log Kd distribution obtained (from 1.05 to 1.91 L Kg−1) indicates that the compounds tend to be adsorbed in sediments, which is probably due to the hydrophobic interactions confirmed by the significant correlations determined mainly between the concentrations and parameters of total organic carbon (TOC), total suspended solids (TSS), biological oxygen demand (BOD5) and chemical oxygen demand (COD). Of five sites analyzed, four presented estrogenic risk due to the analyzed endocrine-disrupting compounds (EEQE2 > 1 ng·L−1).

Evaluation of the Toxicity of Bisphenol A in Reproduction and Its Effect on Fertility and Embryonic Development in the Zebrafish (Danio rerio)

Bisphenol A (BPA) is a chemical substance commonly used in the manufacture of plastic products. Its inhalation or ingestion from particles in suspension, water, and/or polluted foods can trigger toxic effects related to endocrine disruption, resulting in hormonal, reproduction, and immunological alterations in humans and animals. The zebrafish (Danio rerio) is an ideal experimental model frequently used in toxicity studies. In order to assess the toxic effects of BPA on reproduction and embryonic development in one generation after parental exposure to it, a total of 80 zebrafish, males and females, divided into four groups in duplicate (n = 20) were exposed to BPA concentrations of 500, 50, and 5 µg L−1, along with a control group. The fish were kept in reproduction aquariums for 21 days. The embryos obtained in the crosses were incubated in a BPA-free medium and observed for signs of embryotoxicity. A histopathological study (under optical and electron microscopes) was performed of adult fish gonads. The embryos of reproducers exposed to BPA were those most frequently presenting signs of embryotoxicity, such as mortality and cardiac and musculoskeletal malformations. In the histopathological studies of adult individuals, alterations were found in ovocyte maturation and in spermatazoid formation in the groups exposed to the chemical. Those alterations were directly related to BPA action, affecting fertility in both sexes, as well as the viability of their offspring, proportionally to the BPA levels to which they were exposed, so that our results provide more information by associating toxic effects on the offspring and on the next generation.