Evaluation of employing poly-lysine tags versus poly-histidine tags for purification and characterization of recombinant copper-binding proteins

2016 ◽  
Vol 162 ◽  
pp. 286-294 ◽  
Author(s):  
Chathuri J.K. Wijekoon ◽  
Ashwinie A. Ukuwela ◽  
Anthony G. Wedd ◽  
Zhiguang Xiao
Keyword(s):  
Binding Proteins ◽  
Copper Binding ◽  
Purification And Characterization ◽  
Copper Binding Proteins

Rapid induction of copper-binding proteins in the gills of metal exposed mussels

1980 ◽  
Vol 67 (2) ◽  
pp. 215-218 ◽  
Author(s):  
A. Viarengo ◽  
M. Pertica ◽  
G. Mancinelli ◽  
G. Zanicchi ◽  
M. Orunesu
Keyword(s):  
Binding Proteins ◽  
Copper Binding ◽  
Rapid Induction ◽  
Copper Binding Proteins

Zinc- and copper-binding proteins in the plasma of winter flounder (Pseudopleuronectes americanus)

1980 ◽  
Vol 58 (4) ◽  
pp. 609-613 ◽  
Author(s):  
P. E. Fletcher ◽  
G. L. Fletcher
Keyword(s):  
Molecular Weight ◽  
Binding Proteins ◽  
Binding Protein ◽  
Gel Filtration ◽  
Protein S ◽  
Egg Yolk ◽  
Winter Flounder ◽  
Zinc Binding ◽  
Copper Binding ◽  
Copper Binding Proteins

Zinc- and copper-binding proteins were isolated from the plasma of winter flounder using gel filtration chromatography. A single copper-binding protein fraction of molecular weight 170 000 was isolated from the plasma of both sexes.In male and female flounder over 95% of the plasma zinc was associated with a zinc-binding protein(s) with a molecular weight of 76 000. In male flounder the remaining zinc appeared to be bound to a protein(s) of molecular weight 186 000. In female flounder the remaining 5% of the zinc was associated with two zinc-binding fractions with apparent molecular weights of 186 000 and 340 000 – 370 000.Extracts of plasma vitellogenin and egg yolk proteins revealed significant quantities of zinc and copper. It is hypothesized that the female specific zinc-binding protein (340 000 – 370 000) was vitellogenin.

scholarly journals Purification and Characterization of Insulin-like Growth Factor Binding Proteins (IGF-BP) in Amniotic Fluid

1990 ◽  
Vol 66 (6) ◽  
pp. 636-648
Author(s):  
Mitsutoshi IWASHITA ◽  
Masako WATANABE ◽  
Tomoko ADACHI ◽  
Yoshihiko TAKEDA ◽  
Shoichi SAKAMOTO
Keyword(s):  
Growth Factor ◽  
Amniotic Fluid ◽  
Binding Proteins ◽  
Insulin Like Growth Factor ◽  
Purification And Characterization ◽  
Factor Binding

Purification and characterization of Ca2+ /calmodulin-dependent actin-binding proteins from squid retina

FEBS Letters ◽  
1989 ◽  
Vol 247 (2) ◽  
pp. 377-380 ◽  
Author(s):  
Haruo Asai ◽  
Takao Arai ◽  
Toshihiro Fujii ◽  
Gen Matsumoto
Keyword(s):  
Binding Proteins ◽  
Actin Binding ◽  
Purification And Characterization ◽  
Actin Binding Proteins

Stability and Folding of Copper-Binding Proteins

2010 ◽  
pp. 61-80
Author(s):  
Irina Pozdnyakova ◽  
Pernilla Wittung-Stafshede
Keyword(s):  
Binding Proteins ◽  
Copper Binding ◽  
Copper Binding Proteins
Sign in / Sign up

Export Citation Format

Share Document