Development and validation of a specific real-time PCR assay for the detection of the parasite Perkinsus olseni

2020 ◽  
Vol 169 ◽  
pp. 107301 ◽  
Author(s):  
R. Ríos ◽  
R. Aranguren ◽  
M. Gastaldelli ◽  
G. Arcangeli ◽  
B. Novoa ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
Development And Validation

scholarly journals Correction for Hilbert et al., Development and Validation of a Highly Accurate Quantitative Real-Time PCR Assay for Diagnosis of Bacterial Vaginosis

2016 ◽  
Vol 54 (7) ◽  
pp. 1930-1930 ◽  
Author(s):  
David W. Hilbert ◽  
William L. Smith ◽  
Sean G. Chadwick ◽  
Geoffrey Toner ◽  
Eli Mordechai ◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Real Time ◽  
Real Time Pcr ◽  
Quantitative Real Time Pcr ◽  
Pcr Assay ◽  
Development And Validation

Development and validation of a quantitative real-time PCR assay for the early diagnosis of coccidioidomycosis

2014 ◽  
Vol 79 (2) ◽  
pp. 214-221 ◽  
Author(s):  
Sara Gago ◽  
María José Buitrago ◽  
Karl V. Clemons ◽  
Manuel Cuenca-Estrella ◽  
Laurence F. Mirels ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Real Time ◽  
Real Time Pcr ◽  
Quantitative Real Time Pcr ◽  
Pcr Assay ◽  
Development And Validation

scholarly journals Development and Validation of a Real-Time PCR Assay for Rapid Detection of Two-Spotted Spider Mite, Tetranychus urticae (Acari: Tetranychidae)

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0131887 ◽  
Author(s):  
Dongmei Li ◽  
Qing-Hai Fan ◽  
David W. Waite ◽  
Disna Gunawardana ◽  
Sherly George ◽  
...  
Keyword(s):  
Real Time ◽  
Tetranychus Urticae ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Spider Mite ◽  
Pcr Assay ◽  
Two Spotted Spider Mite ◽  
Development And Validation

scholarly journals Development and validation of a real-time PCR assay for the detection of clinical acanthamoebae

2017 ◽  
Vol 10 (1) ◽  
Author(s):  
Nessika Karsenti ◽  
Rachel Lau ◽  
Andrew Purssell ◽  
Ann Chong-Kit ◽  
Marlou Cunanan ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
Development And Validation

scholarly journals Development and validation of a real-time PCR specific for the leukotoxin gene of Bibersteinia trehalosi

2018 ◽  
Vol 30 (4) ◽  
pp. 589-592 ◽  
Author(s):  
Halcyon J. Killion ◽  
William Edwards ◽  
Jessica Jennings-Gaines ◽  
Mary Wood ◽  
Karen Fox ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Indirect Method ◽  
Pcr Primers ◽  
Bighorn Sheep ◽  
Mixed Species ◽  
Pcr Assay ◽  
Field Samples ◽  
Bibersteinia Trehalosi ◽  
Development And Validation

A real-time PCR assay for the leukotoxin gene of Bibersteinia trehalosi was developed and validated to better identify this pathogen, which is a cause of respiratory disease in bighorn sheep. The specificity of the PCR primers was evaluated with DNA from 59 known isolates of the Pasteurellaceae family. For validation, 162 field samples were compared using both the new assay and an indirect method using 2 sets of published protocols. The real-time PCR assay was found to be specific for the leukotoxin gene of B. trehalosi and provides a rapid and direct approach for detecting leukotoxin-producing forms of this organism from samples containing mixed species of leukotoxin-positive Pasteurellaceae.

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