Development and validation of a reverse transcription real‐time PCR assay for specific detection of PRRSGard vaccine‐like virus

2021 ◽  
Author(s):  
Gaurav Rawal ◽  
Wannarat Yim‐im ◽  
Fabian Chamba ◽  
Chad Smith ◽  
Jeff Okones ◽  
...  
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Real Time Pcr ◽  
Specific Detection ◽  
Pcr Assay ◽  
Development And Validation

scholarly journals Development and Validation of a TaqMan Real-Time PCR Assay for the Specific Detection and Quantification of Fusarium fujikuroi in Rice Plants and Seeds

2017 ◽  
Vol 107 (7) ◽  
pp. 885-892 ◽  
Author(s):  
Greice Amaral Carneiro ◽  
Slavica Matić ◽  
Giuseppe Ortu ◽  
Angelo Garibaldi ◽  
Davide Spadaro ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Fusarium Species ◽  
Fusarium Fujikuroi ◽  
Specific Detection ◽  
Pcr Assay ◽  
Bakanae Disease ◽  
Repeatability And Reproducibility ◽  
Development And Validation ◽  
Early Rice

Bakanae disease, which is caused by the seedborne pathogen Fusarium fujikuroi, is found throughout the world on rice. A TaqMan real-time PCR has been developed on the TEF 1-α gene to detect F. fujikuroi in different rice tissues. Three primer/probe sets were tested. The selected set produced an amplicon of 84 bp and was specific for F. fujikuroi with respect to eight Fusarium species of rice and six other rice common pathogens. The assay was validated for specificity, selectivity, sensitivity, repeatability, and reproducibility. The detection limit was set at 27.5 fg of DNA, which is approximately equivalent to one haploid genome of F. fujikuroi. The developed TaqMan real-time assay was able to efficiently detect and quantify F. fujikuroi from rice culms, leaves, roots, and seeds. At 1 week post-germination (wpg), the pathogen was more diffused in the green tissues, while at 3 wpg it was uniformly spread also in the roots. The highest concentration of F. fujikuroi was measured in the M6 cultivar, which showed around 1,450 fungal cells/g. The assay was sufficiently sensitive to detect a few genomic equivalents in the rice seeds, corresponding to 9.89 F. fujikuroi cells/g. The assay permitted bakanae disease to be detected in asymptomatic tissues at the early rice development stages.

scholarly journals A TaqMan-based real-time PCR assay for specific detection of novel duck reovirus in China

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Shuai Zhang ◽  
Weihua Li ◽  
Xiaodong Liu ◽  
Xudong Li ◽  
Bin Gao ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Specific Detection ◽  
Pcr Assay

scholarly journals Development and validation of a SYBR Green real-time PCR assay for rapid and quantitative detection of goose interferons and proinflammatory cytokines

2015 ◽  
Vol 94 (10) ◽  
pp. 2382-2387 ◽  
Author(s):  
Hao Zhou ◽  
Shun Chen ◽  
Yulin Qi ◽  
Mingshu Wang ◽  
Renyong Jia ◽  
...  
Keyword(s):  
Real Time ◽  
Proinflammatory Cytokines ◽  
Real Time Pcr ◽  
Sybr Green ◽  
Quantitative Detection ◽  
Pcr Assay ◽  
Development And Validation

scholarly journals Correction for Hilbert et al., Development and Validation of a Highly Accurate Quantitative Real-Time PCR Assay for Diagnosis of Bacterial Vaginosis

2016 ◽  
Vol 54 (7) ◽  
pp. 1930-1930 ◽  
Author(s):  
David W. Hilbert ◽  
William L. Smith ◽  
Sean G. Chadwick ◽  
Geoffrey Toner ◽  
Eli Mordechai ◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Real Time ◽  
Real Time Pcr ◽  
Quantitative Real Time Pcr ◽  
Pcr Assay ◽  
Development And Validation

TaqMan real-time PCR assay for specific detection of Opisthorchis viverrini DNA in Thai patients with hepatocellular carcinoma and cholangiocarcinoma

2008 ◽  
Vol 119 (2) ◽  
pp. 217-224 ◽  
Author(s):  
Nithikoon Suksumek ◽  
Kawin Leelawat ◽  
Surang Leelawat ◽  
Bruce Russell ◽  
Usa Lek-Uthai
Keyword(s):  
Hepatocellular Carcinoma ◽  
Real Time ◽  
Real Time Pcr ◽  
Opisthorchis Viverrini ◽  
Specific Detection ◽  
Pcr Assay

Development and validation of a specific real-time PCR assay for the detection of the parasite Perkinsus olseni

2020 ◽  
Vol 169 ◽  
pp. 107301 ◽  
Author(s):  
R. Ríos ◽  
R. Aranguren ◽  
M. Gastaldelli ◽  
G. Arcangeli ◽  
B. Novoa ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
Development And Validation

Development of a taqman-based real-time PCR assay for the rapid and specific detection of novel duck- origin goose parvovirus

2017 ◽  
Vol 34 ◽  
pp. 56-58 ◽  
Author(s):  
Jianchang Wang ◽  
Jinfeng Wang ◽  
Yuan Cui ◽  
Huizhu Nan ◽  
Wanzhe Yuan
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Specific Detection ◽  
Pcr Assay ◽  
Goose Parvovirus

Development and validation of a rapid real-time PCR based method for the specific detection of Salmonella on fresh meat

Meat Science ◽  
2009 ◽  
Vol 83 (3) ◽  
pp. 555-562 ◽  
Author(s):  
Sheila McGuinness ◽  
Evonne McCabe ◽  
Edel O’Regan ◽  
Anthony Dolan ◽  
Geraldine Duffy ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Specific Detection ◽  
Fresh Meat ◽  
Development And Validation
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