NMR Structure of the 101-nucleotide Core Encapsidation Signal of the Moloney Murine Leukemia Virus

2004 ◽  
Vol 337 (2) ◽  
pp. 427-442 ◽  
Author(s):  
Victoria D'Souza ◽  
Anwesha Dey ◽  
Dina Habib ◽  
Michael F. Summers
Keyword(s):  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Nmr Structure ◽  
Moloney Murine Leukemia Virus ◽  
Encapsidation Signal ◽  
Murine Leukemia

Three-dimensional 1H NMR structure of the nucleocapsid protein NCp10 of Moloney murine leukemia virus

1994 ◽  
Vol 4 (2) ◽  
pp. 153-170 ◽  
Author(s):  
Hélène Déméné ◽  
Nathalie Jullian ◽  
Nelly Morellet ◽  
Hugues de Rocquigny ◽  
Fabrice Cornille ◽  
...  
Keyword(s):  
1H Nmr ◽  
Murine Leukemia Virus ◽  
Nucleocapsid Protein ◽  
Leukemia Virus ◽  
Three Dimensional ◽  
Nmr Structure ◽  
Moloney Murine Leukemia Virus ◽  
Murine Leukemia

scholarly journals RNA Sequences in the Moloney Murine Leukemia Virus Genome Bound by the Gag Precursor Protein in the Yeast Three-Hybrid System

2004 ◽  
Vol 78 (14) ◽  
pp. 7677-7684 ◽  
Author(s):  
Matthew J. Evans ◽  
Eran Bacharach ◽  
Stephen P. Goff
Keyword(s):  
Hybrid System ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Specific Interaction ◽  
Moloney Murine Leukemia Virus ◽  
Structural Protein ◽  
Encapsidation Signal ◽  
Rna Interaction ◽  
Murine Leukemia

ABSTRACT Encapsidation of the Moloney murine leukemia virus (MMLV) genome is mediated through a specific interaction between the major viral structural protein, Gag, and an RNA packaging signal, Ψ. Many studies have investigated this process in vivo, although the specific examination of the Gag-RNA interaction in this context is difficult due to the variety of other viral functions involved in virion assembly in vivo. The Saccharomyces cerevisiae three-hybrid assay was used to directly examine the interaction between MMLV Gag and Ψ. In this system, MMLV RNA regions exhibiting high-affinity Gag binding were mapped. All Gag-binding regions were located 3′ to the viral splice donor sequence of the viral RNA transcript. No single short RNA sequence within Ψ supported strong Gag interaction. Instead, an RNA comprised of nearly the entire Ψ region was necessary to demonstrate an appreciable Gag interaction in the yeast three-hybrid system. These finding support the notion that two stem-loops (C and D) are not sufficient to form a core MMLV encapsidation signal.

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