DNA-induced Conformational Changes in Type II Restriction Endonucleases: The Structure of Unliganded HincII

2005 ◽  
Vol 351 (1) ◽  
pp. 76-88 ◽  
Author(s):  
Elizabeth J. Little ◽  
Nancy C. Horton
Keyword(s):  
Conformational Changes ◽  
Restriction Endonucleases ◽  
Type Ii

Type II restriction endonucleases fromHelicobacter pyloriinclude an enzyme with a novel recognition sequence

1999 ◽  
Vol 179 (1) ◽  
pp. 175-180 ◽  
Author(s):  
Ana Ivic ◽  
Kenneth J Jakeman ◽  
Charles W Penn ◽  
Nigel L Brown
Keyword(s):  
Restriction Endonucleases ◽  
Type Ii ◽  
Recognition Sequence

scholarly journals The redox environment triggers conformational changes and aggregation of hIAPP in Type II Diabetes

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Diana C. Rodriguez Camargo ◽  
Konstantinos Tripsianes ◽  
Katalin Buday ◽  
Andras Franko ◽  
Christoph Göbl ◽  
...  
Keyword(s):  
Conformational Changes ◽  
Type Ii Diabetes ◽  
Type Ii ◽  
Redox Environment

The pH-Dependent Interaction of Cinnamomin with Lipid Membranes Investigated by Fluorescence Methods

2000 ◽  
Vol 381 (7) ◽  
pp. 567-573 ◽  
Author(s):  
Rong-gui Hu ◽  
Shuang Tang ◽  
Wang-yi Liu
Keyword(s):  
Conformational Changes ◽  
Lipid Membranes ◽  
Type Ii ◽  
Ans Binding ◽  
Tryptophan Residues ◽  
New Type ◽  
Small Unilamellar Vesicles ◽  
Ph Dependent ◽  
Endocytic Vesicles

Abstract Cinnamomin, a new type II ribosomeinactivating protein (RIP), was found to be able to induce the release of calcein loaded in lecithin small unilamellar vesicles and the fusion or aggregation of the lecithin liposomes. Such induction could be promoted several fold by a pH 5.0 environment, a condition similar to that in endocytic vesicles. Lowering the pH from 7.5 to 5.0 evoked conformational changes of cinnamomin and unmasked its hydrophobic areas, including the exposure of 1-anilino-8-naphthalenesulfonate (1,8-ANS) binding sites of the molecule. Some tryptophan residues with affinity to acrylamide were demonstrated to participate in the lipidprotein interaction. The pH dependent fusogenicity of type II RIP might suggest its in vivo function as a fusogen to exert its cytotoxicity.

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