Bsu2413I and Bfi2411I, two new thermophilic type II restriction endonucleases from Bacillus subtilis and Bacillus firmus: isolation and partial purification – Thermophilic endonucleases from two Bacillus species

Changes in the bacterial spectrum of cabbage heads after storage under commonly used storage conditions were examined in this study. Cabbage seeds (Brassica oleracea var. capitata L.) were artificially inoculated with X. campestris pv. campestris (Xcc), a serious pathogen of cruciferous plants causing black rot. Isolation of bacterial cultures from Xcc-inoculated and non-inoculated cabbage heads were carried out in two time points—at the day of harvest and after four months of storage. According to our previous research and literature reports, the most frequent genera of bacteria were chosen for PCR testing, i.e., Bacillus cereus group, Bacillus subtilis group, Pseudomonas sp., and X. campestris pv. campestris. A few of the obtained bacterial cultures were negative for the four above-mentioned species. In those, other bacteria were identified by 16S rRNA sequencing. In both Xcc-inoculated and non-inoculated cabbage heads, changes of the bacterial spectrum over time were observed. The severity of Xcc infection of heads increased after four months of storage. Bacillus species represented the most frequently occurring bacterial genus. The presence of the Bacillus subtilis group increased significantly after storage in non-inoculated cabbage heads. The minor part of the other genera identified by sequencing in the first sampling were not detected in the stored cabbage heads. This was associated with a possible antagonistic behavior of Pseudomonas sp. and Bacillus sp.

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Metal-Dependent Type II Restriction Endonucleases

Handbook of Metalloproteins ◽

10.1002/0470028637.met063 ◽

2006 ◽

Author(s):

��va Scheuring Vanamee ◽

Aneel K Aggarwal

Keyword(s):

Restriction Endonucleases ◽

Type Ii ◽

Dependent Type

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Sequencing, cloning, and heterologous expression of cyclomaltodextrin glucanotransferase of Bacillus firmus strain 37 in Bacillus subtilis WB800

Bioprocess and Biosystems Engineering ◽

10.1007/s00449-018-02068-4 ◽

2019 ◽

Vol 42 (4) ◽

pp. 621-629 ◽

Cited By ~ 1

Author(s):

Gabriela Gregolin Gimenez ◽

Hernán Costa ◽

Quirino Alves de Lima Neto ◽

Maria Aparecida Fernandez ◽

Susana Alicia Ferrarotti ◽

...

Keyword(s):

Bacillus Subtilis ◽

Heterologous Expression ◽

Bacillus Firmus ◽

Cyclomaltodextrin Glucanotransferase

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Physical distance between the site of type II DNA binding to the membrane and oriC on the Bacillus subtilis 168 chromosome.

Journal of Bacteriology ◽

10.1128/jb.174.16.5466-5470.1992 ◽

1992 ◽

Vol 174 (16) ◽

pp. 5466-5470 ◽

Cited By ~ 14

Author(s):

M Itaya ◽

J J Laffan ◽

N Sueoka

Keyword(s):

Bacillus Subtilis ◽

Dna Binding ◽

Physical Distance ◽

Type Ii ◽

Bacillus Subtilis 168

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Toxin–Antitoxin Systems in Bacillus subtilis

Toxins ◽

10.3390/toxins11050262 ◽

2019 ◽

Vol 11 (5) ◽

pp. 262 ◽

Cited By ~ 11

Author(s):

Sabine Brantl ◽

Peter Müller

Keyword(s):

Bacillus Subtilis ◽

Physiological Role ◽

Present Knowledge ◽

The Other ◽

Type I ◽

Type Ii ◽

Free Living ◽

Bacterial Chromosomes ◽

Maintenance Systems ◽

Living Bacteria

Toxin–antitoxin (TA) systems were originally discovered as plasmid maintenance systems in a multitude of free-living bacteria, but were afterwards found to also be widespread in bacterial chromosomes. TA loci comprise two genes, one coding for a stable toxin whose overexpression kills the cell or causes growth stasis, and the other coding for an unstable antitoxin that counteracts toxin action. Of the currently known six types of TA systems, in Bacillus subtilis, so far only type I and type II TA systems were found, all encoded on the chromosome. Here, we review our present knowledge of these systems, the mechanisms of antitoxin and toxin action, and the regulation of their expression, and we discuss their evolution and possible physiological role.

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