Insect Juvenile Hormone Binding Protein Shows Ancestral Fold Present in Human Lipid-Binding Proteins

2008 ◽  
Vol 377 (3) ◽  
pp. 870-881 ◽  
Author(s):  
Robert Kolodziejczyk ◽  
Grzegorz Bujacz ◽  
Michał Jakób ◽  
Andrzej Ożyhar ◽  
Mariusz Jaskolski ◽  
...  
Keyword(s):  
Juvenile Hormone ◽  
Binding Proteins ◽  
Binding Protein ◽  
Lipid Binding ◽  
Hormone Binding ◽  
Juvenile Hormone Binding Protein ◽  
Hormone Binding Protein ◽  
Lipid Binding Proteins

scholarly journals Identification of specific interaction of juvenile hormone binding protein with isocitrate dehydrogenase.

2011 ◽  
Vol 58 (1) ◽  
Author(s):  
Marta Zalewska ◽  
Andrzej Ożyhar ◽  
Marian Kochman
Keyword(s):  
Molecular Weight ◽  
Juvenile Hormone ◽  
Isocitrate Dehydrogenase ◽  
Galleria Mellonella ◽  
Binding Protein ◽  
Specific Interaction ◽  
Lipid Binding ◽  
Hormone Binding ◽  
Juvenile Hormone Binding Protein ◽  
Hormone Binding Protein

Juvenile hormone (JH) is essential for multiple physiological processes: it controls larval development, metamorphosis and adult reproduction. In insect hemolymph more than 99 % of JH is bound to juvenile hormone binding protein (JHBP), which protects JH from degradation by nonspecific hydrolases and serves as a carrier to supply the hormone to the target tissues. In Galleria mellonella hemolymph, JHBP is found in a complex with lipid-binding high molecular weight proteins (HMWP) and this interaction is enhanced in the presence of JH. In this report, we present studies on the interaction of JHBP with low molecular weight proteins (LMWP) in the hemolymph. Using ligand blotting we found that JHBP interacts with a protein of about 44 kDa. To identify the protein that preferentially binds JHBP, a LMWP fraction was applied to a Sepharose-bound JHBP and, after washing, the column was eluted with free JHBP acting as a specific competitor or with carbonic anhydrase as a negative control. The eluted proteins were separated by SDS/PAGE and analyzed by mass spectrometry. Isocitrate dehydrogenase was identified as a component of the supramolecular complex of JHBP with hemolymph proteins.

The juvenile hormone binding protein of silkworm haemolymph: gene and functional analysis

2001 ◽  
Vol 10 (2) ◽  
pp. 147-154 ◽  
Author(s):  
A. M. W. Vermunt ◽  
M. Kamimura ◽  
M. Hirai ◽  
M. Kiuchi ◽  
T. Shiotsuki
Keyword(s):  
Functional Analysis ◽  
Juvenile Hormone ◽  
Binding Protein ◽  
Hormone Binding ◽  
Juvenile Hormone Binding Protein ◽  
Hormone Binding Protein
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