Long-term actions of interleukin-1β on K+, Na+ and Ca2+ channel currents in small, IB4-positive dorsal root ganglion neurons; possible relevance to the etiology of neuropathic pain

2019 ◽  
Vol 332 ◽  
pp. 198-211 ◽  
Author(s):  
Myung-chul Noh ◽  
Patrick L. Stemkowski ◽  
Peter A. Smith
Keyword(s):  
Neuropathic Pain ◽  
Dorsal Root Ganglion ◽  
Dorsal Root ◽  
Dorsal Root Ganglion Neurons ◽  
Interleukin 1Β ◽  
Ganglion Neurons ◽  
Channel Currents

scholarly journals An integrated cell isolation and purification method for rat dorsal root ganglion neurons

2019 ◽  
Vol 47 (7) ◽  
pp. 3253-3260
Author(s):  
Huaishuang Shen ◽  
Minfeng Gan ◽  
Huilin Yang ◽  
Jun Zou
Keyword(s):  
Neuropathic Pain ◽  
Dorsal Root Ganglion ◽  
Primary Culture ◽  
Dorsal Root ◽  
Cell Isolation ◽  
Dorsal Root Ganglion Neurons ◽  
Drg Neurons ◽  
Purification Method ◽  
Isolation And Purification ◽  
Ganglion Neurons

Objective Neurobiology studies are increasingly focused on the dorsal root ganglion (DRG), which plays an important role in neuropathic pain. Existing DRG neuron primary culture methods have considerable limitations, including challenging cell isolation and poor cell yield, which cause difficulty in signaling pathway studies. The present study aimed to establish an integrated primary culture method for DRG neurons. Methods DRGs were obtained from fetal rats by microdissection, and then dissociated with trypsin. The dissociated neurons were treated with 5-fluorouracil to promote growth of neurons from the isolated cells. Then, reverse transcription polymerase chain reaction and immunofluorescence assays were used to identify and purify DRG neurons. Results Isolated DRGs were successfully dissociated and showed robust growth as individual DRG neurons in neurobasal medium. Both mRNA and protein assays confirmed that DRG neurons expressed neurofilament-200 and neuron-specific enolase. Conclusions Highly purified, stable DRG neurons could be easily harvested and grown for extended periods by using this integrated cell isolation and purification method, which may help to elucidate the mechanisms underlying neuropathic pain.

Altered tachykinin expression by dorsal root ganglion neurons in a rat model of neuropathic pain

Pain ◽  
1994 ◽  
Vol 58 (2) ◽  
pp. 219-231 ◽  
Author(s):  
James E. Marchand ◽  
Heinrich W. Wurm ◽  
Toshimasa Kato ◽  
Richard M. Kream
Keyword(s):  
Neuropathic Pain ◽  
Dorsal Root Ganglion ◽  
Rat Model ◽  
Dorsal Root ◽  
Dorsal Root Ganglion Neurons ◽  
Ganglion Neurons
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