NMR analysis and site-specific protonation constants of streptomycin

2012 ◽  
Vol 59 ◽  
pp. 78-82 ◽  
Author(s):  
Gábor Orgován ◽  
Béla Noszál
Keyword(s):  
Protonation Constants ◽  
Nmr Analysis ◽  
Site Specific

scholarly journals 2P116 NMR analysis of HAC1 mRNA recognized by the site-specific endonuclease Ire1p(04. Nucleic acid binding proteins,Poster)

2013 ◽  
Vol 53 (supplement1-2) ◽  
pp. S178
Author(s):  
Ikumi Kawahara ◽  
Yuta Ashihara ◽  
Kaichiro Haruta ◽  
Yoshiyuki Tanaka ◽  
Chojiro Kojima
Keyword(s):  
Nucleic Acid ◽  
Binding Proteins ◽  
Nucleic Acid Binding ◽  
Nmr Analysis ◽  
Site Specific ◽  
Nucleic Acid Binding Proteins

NMR Analysis of Site-Specific Ligand Binding in Oligomeric Proteins. Dynamic Studies on the Interaction of Riboflavin Synthase with Trifluoromethyl-Substituted Intermediates†

Biochemistry ◽  
1996 ◽  
Vol 35 (30) ◽  
pp. 9637-9646 ◽  
Author(s):  
Johannes Scheuring ◽  
Markus Fischer ◽  
Mark Cushman ◽  
John Lee ◽  
Adelbert Bacher ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Nmr Analysis ◽  
Site Specific ◽  
Oligomeric Proteins ◽  
Dynamic Studies ◽  
Specific Ligand

scholarly journals Site-Specific Isotope-Labeling of Inosine Phosphoramidites and NMR Analysis of an Inosine-Containing RNA Duplex

2016 ◽  
Vol 22 (43) ◽  
pp. 15350-15359 ◽  
Author(s):  
Andre Dallmann ◽  
Alexander V. Beribisky ◽  
Felix Gnerlich ◽  
Martin Rübbelke ◽  
Stefan Schiesser ◽  
...  
Keyword(s):  
Isotope Labeling ◽  
Nmr Analysis ◽  
Site Specific

scholarly journals Synthesis and NMR analysis of 13C and 15N-labeled long-chain polyamines (LCPAs)

RSC Advances ◽  
2016 ◽  
Vol 6 (96) ◽  
pp. 93343-93348 ◽  
Author(s):  
Maryna Abacilar ◽  
Fabian Daus ◽  
Christian Haas ◽  
Stephan Ingmar Brückner ◽  
Eike Brunner ◽  
...  
Keyword(s):  
Nmr Analysis ◽  
Site Specific ◽  
Silica Nanocomposites ◽  
Long Chain

Site specific isotope labels in long-chain polyamines (LCPAs) for the NMR analysis of bioinspired silica nanocomposites.

scholarly journals NMR analysis of site-specific mutants of yeast phosphoglycerate kinase. An investigation of the triose-binding site

1989 ◽  
Vol 183 (1) ◽  
pp. 57-67 ◽  
Author(s):  
Wayne J. FAIRBROTHER ◽  
Philip A. WALKER ◽  
Philippe MINARD ◽  
Jennifer A. LITTLECHILD ◽  
Herman C. WATSON ◽  
...  
Keyword(s):  
Binding Site ◽  
Phosphoglycerate Kinase ◽  
Nmr Analysis ◽  
Site Specific

Large-cluster and combined fluorescent and gold immunoprobes

1996 ◽  
Vol 54 ◽  
pp. 892-893
Author(s):  
Richard D. Powell ◽  
James F. Hainfeld ◽  
Carol M. R. Halsey ◽  
David L. Spector ◽  
Shelley Kaurin ◽  
...  
Keyword(s):  
Metal Cluster ◽  
Sulfonyl Chloride ◽  
Gel Filtration ◽  
Cross Linking ◽  
Site Specific ◽  
Fab Fragments ◽  
Cluster Label ◽  
Covalently Linked ◽  
Texas Red ◽  
Fold Excess

Two new types of covalently linked, site-specific immunoprobes have been prepared using metal cluster labels, and used to stain components of cells. Combined fluorescein and 1.4 nm “Nanogold” labels were prepared by using the fluorescein-conjugated tris (aryl) phosphine ligand and the amino-substituted ligand in the synthesis of the Nanogold cluster. This cluster label was activated by reaction with a 60-fold excess of (sulfo-Succinimidyl-4-N-maleiniido-cyclohexane-l-carboxylate (sulfo-SMCC) at pH 7.5, separated from excess cross-linking reagent by gel filtration, and mixed in ten-fold excess with Goat Fab’ fragments against mouse IgG (obtained by reduction of F(ab’)2 fragments with 50 mM mercaptoethylamine hydrochloride). Labeled Fab’ fragments were isolated by gel filtration HPLC (Superose-12, Pharmacia). A combined Nanogold and Texas Red label was also prepared, using a Nanogold cluster derivatized with both and its protected analog: the cluster was reacted with an eight-fold excess of Texas Red sulfonyl chloride at pH 9.0, separated from excess Texas Red by gel filtration, then deprotected with HC1 in methanol to yield the amino-substituted label.

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