Improved detection of variants in recombinant human interferon alpha-2a products by reverse-phase high-performance liquid chromatography on a core–shell stationary phase

2014 ◽  
Vol 88 ◽  
pp. 123-129 ◽  
Author(s):  
Yonghong Li ◽  
Chunming Rao ◽  
Lei Tao ◽  
Junzhi Wang ◽  
Barry Lorbetskie ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Stationary Phase ◽  
Interferon Alpha ◽  
High Performance ◽  
Human Interferon ◽  
Core Shell ◽  
Reverse Phase

MOF@SiO 2 core-shell composites as stationary phase in high performance liquid chromatography

2018 ◽  
Vol 263 ◽  
pp. 268-274 ◽  
Author(s):  
Sebastian Ehrling ◽  
Christel Kutzscher ◽  
Pascal Freund ◽  
Philipp Müller ◽  
Irena Senkovska ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Stationary Phase ◽  
High Performance ◽  
Core Shell

Magnetic Microparticles@UiO-67 Core-Shell Composites as a Novel Stationary Phase for High Performance Liquid Chromatography

2014 ◽  
Vol 703 ◽  
pp. 73-76 ◽  
Author(s):  
Wei Wei Qin ◽  
Martin E. Silvestre ◽  
Matthias Franzreb
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Stationary Phase ◽  
High Performance ◽  
Separation Efficiency ◽  
Core Shell ◽  
Moderate Pressure ◽  
Fast Kinetics ◽  
Phenol Derivatives ◽  
Magnetic Silica

Metal-organic framework UiO-67 was explored as a novel stationary phase for high performance liquid chromatography (HPLC). UiO-67 was, for the first time, homogeneously coated on carboxyl functionalized magnetic silica microparticles at low temperature (45 °C) by using a recently introduced liquid phase epitaxy (LPE) process. HPLC runs using the synthesized core-shell microparticles as stationary phase showed baseline separation for three phenol derivatives, applying gradient elution using acetonitrile and water as mobile phase. It also showed that UiO-67 has the largest affinity for 2,6-dichlorophenol among the phenol derivatives tested. The comparison of core-shell microparticles with 20 and 55 layers, respectively, of UiO-67 grown on the magnetic silica core proof that the UiO-67 shell determines separation behavior. Therefore, the use of UiO-67 core-shell microparticles as a stationary phase combines the advantages of a thin, homogenous MOF shell showing fast kinetics and good separation efficiency with the advantages of spherical silica microparticle cores offering high mechanical robustness and moderate pressure drop.

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