Hydrogen Sulfide Protects Against Ischemia-Reperfusion Injury in an In Vitro Model of Cutaneous Tissue Transplantation

2010 ◽  
Vol 159 (1) ◽  
pp. 451-455 ◽  
Author(s):  
Peter W. Henderson ◽  
Sunil P. Singh ◽  
Daniel Belkin ◽  
Vamsi Nagineni ◽  
Andrew L. Weinstein ◽  
...  
2011 ◽  
Vol 165 (2) ◽  
pp. 281-282
Author(s):  
B.J. DuBray ◽  
K.D. Conzen ◽  
G.A. Upadhya ◽  
P. Balachandran ◽  
J. Jia ◽  
...  

Surgery ◽  
2012 ◽  
Vol 152 (2) ◽  
pp. 247-253
Author(s):  
Bernard J. DuBray ◽  
Kendra D. Conzen ◽  
Gundumi A. Upadhya ◽  
Parvathi Balachandran ◽  
Jianluo Jia ◽  
...  

2021 ◽  
Vol 22 (2) ◽  
pp. 510
Author(s):  
Vitale Miceli ◽  
Alessandro Bertani ◽  
Cinzia Maria Chinnici ◽  
Matteo Bulati ◽  
Mariangela Pampalone ◽  
...  

The clinical results of lung transplantation (LTx) are still less favorable than other solid organ transplants in both the early and long term. The fragility of the lungs limits the procurement rate and can favor the occurrence of ischemia-reperfusion injury (IRI). Ex vivo lung perfusion (EVLP) with Steen SolutionTM (SS) aims to address problems, and the implementation of EVLP to alleviate the activation of IRI-mediated processes has been achieved using mesenchymal stromal/stem cell (MSC)-based treatments. In this study, we investigated the paracrine effects of human amnion-derived MSCs (hAMSCs) in an in vitro model of lung IRI that includes cold ischemia and normothermic EVLP. We found that SS enriched by a hAMSC-conditioned medium (hAMSC-CM) preserved the viability and delayed the apoptosis of alveolar epithelial cells (A549) through the downregulation of inflammatory factors and the upregulation of antiapoptotic factors. These effects were more evident using the CM of 3D hAMSC cultures, which contained an increased amount of immunosuppressive and growth factors compared to both 2D cultures and encapsulated-hAMSCs. To conclude, we demonstrated an in vitro model of lung IRI and provided evidence that a hAMSC-CM attenuated IRI effects by improving the efficacy of EVLP, leading to strategies for a potential implementation of this technique.


2012 ◽  
Vol 31 (2) ◽  
pp. 437-446 ◽  
Author(s):  
KATALIN MÓDIS ◽  
DOMOKOS GERŐ ◽  
RITA STANGL ◽  
OLIVÉR ROSERO ◽  
ATTILA SZIJÁRTÓ ◽  
...  

2010 ◽  
Vol 126 (6) ◽  
pp. 1890-1898 ◽  
Author(s):  
Peter W. Henderson ◽  
Sunil P. Singh ◽  
Andrew L. Weinstein ◽  
Vijay Nagineni ◽  
Daniel C. Rafii ◽  
...  

2009 ◽  
Vol 124 (Supplement) ◽  
pp. 685-686
Author(s):  
D JM Kadouch ◽  
P W Henderson ◽  
V Nagineni ◽  
A L Weinstein ◽  
S P Singh ◽  
...  

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