Differentiation of infectious bursal disease virus strains using real-time RT-PCR and high resolution melt curve analysis

2011 ◽  
Vol 171 (1) ◽  
pp. 264-271 ◽  
Author(s):  
Seyed A. Ghorashi ◽  
Denise O’Rourke ◽  
Jagoda Ignjatovic ◽  
Amir H. Noormohammadi
Keyword(s):  
High Resolution ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Curve Analysis ◽  
Infectious Bursal Disease ◽  
Rt Pcr ◽  
High Resolution Melt ◽  
Melt Curve Analysis ◽  
Bursal Disease ◽  
Virus Strains

scholarly journals Partial VP1 sequencing of Brazilian infectious bursal disease virus strains

2012 ◽  
Vol 43 (3) ◽  
pp. 1015-1021 ◽  
Author(s):  
Maria Judite Bittencourt Fernandes ◽  
Isabela Cristina Simoni ◽  
Ricardo Harakava ◽  
Eliana Borges Rivas ◽  
Clarice Weis Arns
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Bursal Disease ◽  
Virus Strains

A Practical Tissue Sampling Method Using Ordinary Paper for Molecular Detection of Infectious Bursal Disease Virus RNA by RT-PCR

2006 ◽  
Vol 50 (4) ◽  
pp. 556-560 ◽  
Author(s):  
Min Thein Maw ◽  
Tsuyoshi Yamaguchi ◽  
Christopher J. Kasanga ◽  
Kaori Terasaki ◽  
Hideto Fukushi
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Sampling Method ◽  
Molecular Detection ◽  
Infectious Bursal Disease ◽  
Tissue Sampling ◽  
Rt Pcr ◽  
Virus Rna ◽  
Bursal Disease

scholarly journals Effective inhibition of infectious bursal disease virus replication by recombinant avian adeno-associated virus-delivered microRNAs

2009 ◽  
Vol 90 (6) ◽  
pp. 1417-1422 ◽  
Author(s):  
Yongjuan Wang ◽  
Huaichang Sun ◽  
Pengpeng Shen ◽  
Xinyu Zhang ◽  
Xiaoli Xia
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Viral Vector ◽  
Infectious Bursal Disease ◽  
Inhibitory Effects ◽  
Rt Pcr ◽  
Adeno Associated Virus ◽  
Rnai Technology ◽  
Bursal Disease ◽  
In Cells

RNA interference (RNAi) is a novel antiviral strategy against a variety of virus infections. Infectious bursal disease virus (IBDV) causes an economically important disease in young chickens. This study demonstrated efficient inhibition of IBDV replication by recombinant avian adeno-associated virus (rAAAV)-delivered anti-VP1 and anti-VP2 microRNAs (miRNAs). In the viral vector-transduced cells, sequence-specific miRNA expression was detected by poly(A)-tailed RT-PCR. Reporter assays using a pVP2-EGFP vector showed significant and long-lasting inhibition of VP2–EGFP expression in cells transduced with anti-VP2 miRNA-expressing rAAAV-RFPmiVP2E, but not with the control miRNA-expressing rAAAV-RFPmiVP2con or anti-VP1 miRNA-expressing rAAAV-RFPmiVP1. Semi-quantitative RT-PCR and/or virus titration assays showed a significant inhibitory effect on homologous IBDV replication in cells transduced with rAAAV-RFPmiVP1 or rAAAV-RFPmiVP2E. For two heterologous IBDV isolates, transduction with rAAAV-RFPmiVP1 led to slightly weaker but similar inhibitory effects, whereas transduction with rAAAV-RFPmiVP2E resulted in significantly weaker and different inhibitory effects. These results suggest that rAAAV could act as an efficient vector for miRNA delivery into avian cells and that VP1 is the more suitable target for interfering with IBDV replication using RNAi technology.

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