A multiplex RT-PCR for rapid and simultaneous detection of porcine teschovirus, classical swine fever virus, and porcine reproductive and respiratory syndrome virus in clinical specimens

2011 ◽  
Vol 172 (1-2) ◽  
pp. 88-92 ◽  
Author(s):  
Shanshan Liu ◽  
Yarong Zhao ◽  
Quanbo Hu ◽  
Chaochao Lv ◽  
Chaofan Zhang ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Simultaneous Detection ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Respiratory Syndrome Virus ◽  
Rt Pcr ◽  
Clinical Specimens ◽  
Porcine Teschovirus

A multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, highly pathogenic porcine reproductive and respiratory syndrome virus, porcine reproductive and respiratory syndrome virus and pseudorabies in swines

2015 ◽  
Vol 18 (4) ◽  
pp. 715-723 ◽  
Author(s):  
L. Hu ◽  
X.Y. Lin ◽  
Z.X. Yang ◽  
X.P. Yao ◽  
G.L. Li ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Classical Swine Fever Virus ◽  
African Swine Fever Virus ◽  
Simultaneous Detection ◽  
African Swine Fever ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Respiratory Syndrome Virus ◽  
Multiple Infections ◽  
Highly Pathogenic

Abstract In this assay, we developed and evaluated a multiplex PCR (mPCR) for its ability in detecting multiple infections of swine simultaneously. Four pairs of primers were used to detect five viruses. Specific primers were designed for classical swine fever virus (CSFV), African swine fever virus (ASFV) and pseudorabies (PRV). A pair of primers was designed prudently for two different types of porcine reproductive and respiratory syndrome virus that respectively were porcine reproductive and respiratory syndrome virus (PRRSV), highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV). The detection limits of the mPCR were 1.09×104, 1.50×103, 2.10×103, 1.30×103 and 8.97×102 copies/reaction for CSFV, ASFV, HP-PRRSV, PRRSV and PRV, respectively. A total of 49 clinical specimens were tested by the mPCR, and the result showed that co-infection by two or three viruses was 51%. In conclusion, the PCR is a useful tool for clinical diagnosis of not only single infections but also mixed infections in swines.

Validation of two commercial real-time RT-PCR kits for rapid and specific diagnosis of classical swine fever virus

2008 ◽  
Vol 147 (1) ◽  
pp. 136-142 ◽  
Author(s):  
M. Le Dimna ◽  
R. Vrancken ◽  
F. Koenen ◽  
S. Bougeard ◽  
A. Mesplède ◽  
...  
Keyword(s):  
Real Time ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Rt Pcr ◽  
Specific Diagnosis
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