Multiplex PCR for the simultaneous detection of porcine reproductive and respiratory syndrome virus, classical swine fever virus, and porcine circovirus in pigs

2013 ◽  
Vol 27 (3-4) ◽  
pp. 149-152 ◽  
Author(s):  
Jian-Kui Liu ◽  
Chun-Hua Wei ◽  
Xiao-Yan Yang ◽  
Ai-Ling Dai ◽  
Xiao-Hua Li
Keyword(s):  
Multiplex Pcr ◽  
Classical Swine Fever Virus ◽  
Simultaneous Detection ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Porcine Circovirus ◽  
Respiratory Syndrome Virus

A multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, highly pathogenic porcine reproductive and respiratory syndrome virus, porcine reproductive and respiratory syndrome virus and pseudorabies in swines

2015 ◽  
Vol 18 (4) ◽  
pp. 715-723 ◽  
Author(s):  
L. Hu ◽  
X.Y. Lin ◽  
Z.X. Yang ◽  
X.P. Yao ◽  
G.L. Li ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Classical Swine Fever Virus ◽  
African Swine Fever Virus ◽  
Simultaneous Detection ◽  
African Swine Fever ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Respiratory Syndrome Virus ◽  
Multiple Infections ◽  
Highly Pathogenic

Abstract In this assay, we developed and evaluated a multiplex PCR (mPCR) for its ability in detecting multiple infections of swine simultaneously. Four pairs of primers were used to detect five viruses. Specific primers were designed for classical swine fever virus (CSFV), African swine fever virus (ASFV) and pseudorabies (PRV). A pair of primers was designed prudently for two different types of porcine reproductive and respiratory syndrome virus that respectively were porcine reproductive and respiratory syndrome virus (PRRSV), highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV). The detection limits of the mPCR were 1.09×104, 1.50×103, 2.10×103, 1.30×103 and 8.97×102 copies/reaction for CSFV, ASFV, HP-PRRSV, PRRSV and PRV, respectively. A total of 49 clinical specimens were tested by the mPCR, and the result showed that co-infection by two or three viruses was 51%. In conclusion, the PCR is a useful tool for clinical diagnosis of not only single infections but also mixed infections in swines.

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