A one-step, gel-based RT-PCR assay with comparable performance to real-time RT-PCR for detection of classical swine fever virus

2007 ◽  
Vol 139 (2) ◽  
pp. 203-207 ◽  
Author(s):  
Lihong Liu ◽  
Frederik Widén ◽  
Claudia Baule ◽  
Sándor Belák
Keyword(s):  
Real Time ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Comparable Performance ◽  
One Step

Development and application of real-time TaqMan RT-PCR assay for improved detection of classical swine fever virus in slaughtered pigs

2016 ◽  
Vol 50 (6) ◽  
Author(s):  
M. Rout ◽  
G. Saikumar
Keyword(s):  
Real Time ◽  
Classical Swine Fever Virus ◽  
Clinical Signs ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Taqman Probe ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Fluorogenic Probe ◽  
Slaughtered Pigs

Classical swine fever (CSF) is an economically devastating disease of pigs. Instrumental to the control of CSF is a well-characterized sensitive assay that can deliver a rapid and accurate diagnosis before the onset of clinical signs. With this objective, a real-time fluorogenic-probe hydrolysis (TaqMan) reverse transcription-polymerase chain reaction (RT-PCR) assay was developed for rapid and specific detection of classical swine fever virus (CSFV) and applied on samples derived from infected slaughtered pigs. A pair of PCR primers targeting 5’ -non-coding region (CSFL1 and CSFR1) in conjunction with a CSFV-specific fluorogenic probe (CSFP1) was designed and assessed in real-time PCR. During PCR, when the target of interest was present, the CSFV specific FAM-labeled TaqMan probe annealed to the amplicon between the forward and reverse primers and was subsequently cleaved via the 5¢-3¢ exonuclease activity of the DNA polymerase resulting in the release of the fluorescent reporter dye. This assay was found to be rapid and strain-specific for CSFV detection.

Validation of two commercial real-time RT-PCR kits for rapid and specific diagnosis of classical swine fever virus

2008 ◽  
Vol 147 (1) ◽  
pp. 136-142 ◽  
Author(s):  
M. Le Dimna ◽  
R. Vrancken ◽  
F. Koenen ◽  
S. Bougeard ◽  
A. Mesplède ◽  
...  
Keyword(s):  
Real Time ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Rt Pcr ◽  
Specific Diagnosis

Differentiation of C-strain “Riems” or CP7_E2alf vaccinated animals from animals infected by classical swine fever virus field strains using real-time RT-PCR

2009 ◽  
Vol 158 (1-2) ◽  
pp. 114-122 ◽  
Author(s):  
Immanuel Leifer ◽  
Klaus Depner ◽  
Sandra Blome ◽  
Marie-Frederique Le Potier ◽  
Mireille Le Dimna ◽  
...  
Keyword(s):  
Real Time ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Rt Pcr

Evaluation of a primer-probe energy transfer real-time PCR assay for detection of classical swine fever virus

2010 ◽  
Vol 168 (1-2) ◽  
pp. 259-261 ◽  
Author(s):  
Xing-Juan Zhang ◽  
Hongyan Xia ◽  
Helen Everett ◽  
Olubukola Sosan ◽  
Helen Crooke ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Real Time ◽  
Classical Swine Fever Virus ◽  
Real Time Pcr ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Pcr Assay
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