An optimized expression vector for improving the yield of dengue virus-like particles from transfected insect cells

2014 ◽  
Vol 205 ◽  
pp. 116-123 ◽  
Author(s):  
Nicha Charoensri ◽  
Amporn Suphatrakul ◽  
Rungtawan Sriburi ◽  
Thippawan Yasanga ◽  
Jiraphan Junjhon ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Expression Vector ◽  
Insect Cells ◽  
Virus Like Particles

A high-level expression vector containing selectable marker for continuous production of recombinant protein in insect cells

2009 ◽  
Vol 31 (5) ◽  
pp. 623-627 ◽  
Author(s):  
Takuya Shishido ◽  
Naoya Kurata ◽  
Myung Eui Yoon ◽  
Tsutomu Tanaka ◽  
Hideki Yamaji ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Expression Vector ◽  
Insect Cells ◽  
Selectable Marker ◽  
Continuous Production ◽  
High Level Expression ◽  
High Level

Diagnostic aptitude of West Nile virus-like particles expressed in insect cells

2018 ◽  
Vol 91 (3) ◽  
pp. 233-238 ◽  
Author(s):  
Belén Rebollo ◽  
Javier Sarraseca ◽  
Mª José Rodríguez ◽  
Antonio Sanz ◽  
Miguel Ángel Jiménez-Clavero ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Insect Cells ◽  
West Nile ◽  
Virus Like Particles

scholarly journals Protein requirements for assembly of virus-like particles of Junonia coenia densovirus in insect cells

2000 ◽  
Vol 81 (6) ◽  
pp. 1605-1613 ◽  
Author(s):  
Liliane Croizier ◽  
Max Bergoin ◽  
Jean-Claude Veyrunes ◽  
Guy Croizier ◽  
Miguel López-Ferber ◽  
...  
Keyword(s):  
Insect Cells ◽  
Junonia Coenia ◽  
Virus Like Particles ◽  
Protein Requirements

scholarly journals Expression of GA Coat Protein-Derived Mosaic Virus-Like Particles in Saccharomyces cerevisiae and Packaging in vivo of mRNAs into Particles

2012 ◽  
Vol 66 (6) ◽  
pp. 234-241
Author(s):  
Arnis Strods ◽  
Dagnija Argule ◽  
Indulis Cielens ◽  
Ludmila Jackeviča ◽  
Regīna Renhofa
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Coat Protein ◽  
Pichia Pastoris ◽  
Mosaic Virus ◽  
Expression Vector ◽  
Expression Systems ◽  
Coding Sequences ◽  
Yeast Pichia Pastoris ◽  
Virus Like Particles

Our previous research showed that the best yield of virus-like particles (VLPs) formed by RNA bacteriophage GA coat protein was obtained by expression in yeast Pichia pastoris, while other used expression systems in Saccharomyces cerevisiae gave much lower amounts of capsids. The main reasons to attempt further studies in Saccharomyces cerevisiae were to improve the yield of GA-based VLPs using constructs with optimised nucleotide triplets in coding sequences, and to exploit the possibilities of the two-promoter Gal1/Gal10 system of expression vector pESC-URA for production of the desired mosaic VLPs and for packaging of mRNAs into VLPs in vivo

scholarly journals Immunogenicity in Rabbits of Virus-Like Particles from a Contemporary Rabbit Haemorrhagic Disease Virus Type 2 (GI.2/RHDV2/b) Isolated in The Netherlands

Viruses ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 553 ◽  
Author(s):  
Qiuhong Miao ◽  
Ruibing Qi ◽  
Luut Veldkamp ◽  
Jooske Ijzer ◽  
Marja L. Kik ◽  
...  
Keyword(s):  
The Netherlands ◽  
Disease Virus ◽  
Genome Sequence ◽  
Insect Cells ◽  
Rabbit Haemorrhagic Disease Virus ◽  
Virus Like Particles ◽  
Rabbit Haemorrhagic Disease ◽  
Domestic Rabbits ◽  
Haemorrhagic Disease

Rabbit haemorrhagic disease virus (RHDV) type 2 (GI.2/RHDV2/b) is an emerging pathogen in wild rabbits and in domestic rabbits vaccinated against RHDV (GI.1). Here we report the genome sequence of a contemporary RHDV2 isolate from the Netherlands and investigate the immunogenicity of virus-like particles (VLPs) produced in insect cells. RHDV2 RNA was isolated from the liver of a naturally infected wild rabbit and the complete viral genome sequence was assembled from sequenced RT-PCR products. Phylogenetic analysis based on the VP60 capsid gene demonstrated that the RHDV2 NL2016 isolate clustered with other contemporary RHDV2 strains. The VP60 gene was cloned in a baculovirus expression vector to produce VLPs in Sf9 insect cells. Density-gradient purified RHDV2 VLPs were visualized by transmission electron microscopy as spherical particles of around 30 nm in diameter with a morphology resembling authentic RHDV. Immunization of rabbits with RHDV2 VLPs resulted in high production of serum antibodies against VP60, and the production of cytokines (IFN-γ and IL-4) was significantly elevated in the immunized rabbits compared to the control group. The results demonstrate that the recombinant RHDV2 VLPs are highly immunogenic and may find applications in serological detection assays and might be further developed as a vaccine candidate to protect domestic rabbits against RHDV2 infection.

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