scholarly journals Comparison between dot-immunoblotting assay and clinical sign determination method for quantifying avian infectious bronchitis virus vaccine by titration in embryonated eggs

2016 ◽  
Vol 230 ◽  
pp. 13-17 ◽  
Author(s):  
Seong-su Yuk ◽  
Jung-Hoon Kwon ◽  
Jin-Yong Noh ◽  
Woo-tack Hong ◽  
Gyeong-Bin Gwon ◽  
...  
2018 ◽  
Vol 119 ◽  
pp. 119-124 ◽  
Author(s):  
Javaid Jeelani Matoo ◽  
Khalid Bashir ◽  
Ajay Kumar ◽  
Narayanan Krishnaswamy ◽  
Sohini Dey ◽  
...  

1998 ◽  
Vol 26 (5) ◽  
pp. 629-634
Author(s):  
Emiliana Falcone ◽  
Edoardo Vignolo ◽  
Livia Di Trani ◽  
Simona Puzelli ◽  
Maria Tollis

A reverse transcriptase polymerase chain reaction (RT-PCR) assay specific for identifying avian infectious bronchitis virus (IBV) in poultry vaccines, and the serological response to IBV induced by the inoculation of chicks with a Newcastle disease vaccine spiked with the Massachusetts strain of IBV, were compared for their ability to detect IBV as a contaminant of avian vaccines. The sensitivity of the IBV-RT-PCR assay provided results which were at least equivalent to the biological effect produced by the inoculation of chicks, allowing this assay to be considered a valid alternative to animal testing in the quality control of avian immunologicals. This procedure can easily be adapted to detect a number of contaminants for which the in vivo test still represents the only available method of detection.


2000 ◽  
Vol 146 (7) ◽  
pp. 191-193 ◽  
Author(s):  
A. Zanella ◽  
R. Coaro ◽  
R. Marchi ◽  
G. Fabris ◽  
A. Lavazza

1987 ◽  
Vol 66 (4) ◽  
pp. 765-767 ◽  
Author(s):  
MOHAMMAD A. MUNEER ◽  
JOHN A. NEWMAN ◽  
SAGAR M. GOYAL ◽  
M. AJMAL

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