Single-tubule RNA-Seq uncovers signaling mechanisms that defend against hyponatremia in SIADH

Cycloastragenol (CAG), a molecule isolated from ‘Astragalus membranaceus’, stimulates the telomerase activity and cell proliferation significantly. It has been proven that CAG has the ability to prevent some diseases in humans. In this study, we aimed to figure out the CAG effects on the different signaling mechanisms in plants and to broadly analyze the genome-wide transcriptional responses in order to demonstrate CAG as a new key molecule that can potentially help plants to overcome different environmental stresses. RNA-seq strategy was employed to assess the transcriptional profiles in A. thaliana calli. Our work primarily focused on an overall study on the transcriptomic responses of A. thaliana to CAG. A total of 22593 unigenes have been detected, among which 1045 unigenes associated with 213 GO terms were differentially expressed and were assigned to 118 KEGG pathways. The up-regulated genes are principally involved in cellular and metabolic processes in addition to the response to a stimulus. The data analysis revealed genes associated with defense signaling pathways such as cytochrome P450s transporter, antioxidant system genes, and stress-responsive protein families were significantly upregulated. The obtained results can potentially help in better understanding biotic and/or abiotic tolerance mechanisms in response to CAG.

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New Genomic Approaches to Enhance Biomass Degradation by the Industrial FungusTrichoderma reesei

International Journal of Genomics ◽

10.1155/2018/1974151 ◽

2018 ◽

Vol 2018 ◽

pp. 1-17 ◽

Cited By ~ 10

Author(s):

Renato Graciano de Paula ◽

Amanda Cristina Campos Antoniêto ◽

Liliane Fraga Costa Ribeiro ◽

Cláudia Batista Carraro ◽

Karoline Maria Vieira Nogueira ◽

...

Keyword(s):

Industrial Production ◽

Functional Characterization ◽

Carbon Sources ◽

Biotechnology Industry ◽

Rna Seq ◽

Biomass Degradation ◽

Cellulolytic Microorganisms ◽

Signaling Mechanisms ◽

Analytic Review ◽

Cellulase Expression

The filamentous fungiTrichoderma reeseiis one of the most well-studied cellulolytic microorganisms. It is the most important fungus for the industrial production of enzymes to biomass deconstruction being widely used in the biotechnology industry, mainly in the production of biofuels. Here, we performed an analytic review of the holocellulolytic system presented byT. reeseias well as the transcriptional and signaling mechanisms involved with holocellulase expression in this fungus. We also discuss new perspectives about control of secretion and cellulase expression based on RNA-seq and functional characterization data ofT. reeseigrowth in different carbon sources, which comprise glucose, cellulose, sophorose, and sugarcane bagasse.

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