Direct ex vivo expansion of hematopoietic stem cells from umbilical cord blood on membranes

2010 ◽  
Vol 351 (1-2) ◽  
pp. 104-111 ◽  
Author(s):  
Akon Higuchi ◽  
Siou-Ting Yang ◽  
Pei-Tsz Li ◽  
Miho Tamai ◽  
Yoh-ichi Tagawa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Ex Vivo ◽  
Ex Vivo Expansion ◽  
Hematopoietic Stem

Ex-vivo expansion of hematopoietic stem cells from umbilical cord blood

2011 ◽  
Author(s):  
Claudia Lobato da Silva ◽  
Pedro Z. Andrade ◽  
Francisco dos Santos ◽  
Joaquim M. S. Cabral
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Ex Vivo ◽  
Ex Vivo Expansion ◽  
Hematopoietic Stem

scholarly journals Isolation and <i> Ex Vivo</i> Expansion of Human Hematopoietic Stem Cells Derived from Umbilical Cord Blood

2021 ◽  
Vol 11 (01) ◽  
pp. 1-13
Author(s):  
Mao Pang ◽  
Shiqian Han ◽  
Meng Ren ◽  
Dongwei Guan ◽  
Tianying Kuang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Ex Vivo ◽  
Hematopoietic Stem

scholarly journals Recombinant TAT-BMI-1 fusion protein induces ex vivo expansion of human umbilical cord blood-derived hematopoietic stem cells

Oncotarget ◽  
2017 ◽  
Vol 8 (27) ◽  
pp. 43782-43798 ◽  
Author(s):  
Bruna Codispoti ◽  
Nicola Rinaldo ◽  
Emanuela Chiarella ◽  
Michela Lupia ◽  
Cristina Barbara Spoleti ◽  
...  
Keyword(s):  
Stem Cells ◽  
Fusion Protein ◽  
Hematopoietic Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Ex Vivo ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Hematopoietic Stem

scholarly journals Effect of Stemregenin1 and SB431542 small molecules on ex vivo expansion of umbilical cord blood hematopoietic stem cells on biocompatible polyethersulfone nanofiber scaffolds

2019 ◽  
Author(s):  
Sorush Niknamian
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Cord Blood ◽  
Small Molecules ◽  
Umbilical Cord ◽  
Colony Formation ◽  
Ex Vivo ◽  
Medical Application ◽  
Ex Vivo Expansion ◽  
Hematopoietic Stem

Cord blood Hematopoietic stem cells (HSCs) with several advantages including low chance of viral contamination and low rate of Graft versus host disease (GVHD) are appropriate candidate for vast medical applications such as transplantation. The main obstacle of cord blood HSCs is the low number cells. To improve ex-vivo expansion of umbilical cord HSCs we introduced a new culture system. Isolated HSCs were seeded in Three-dimensional(3D) on Polyethersulfone(PES) scaffolds and Two-dimensional(2D) culture conditions and treated with SB431542 and Stemregenin1(SR1) small molecules. On the fifth and tenth days the expanded cells in different groups were investigated for expression of specific markers by flow cytometry, expression of some stemness genes by qRT-PCR and colony formation by methocult medium. SR1 molecule significantly increased expansion of CD34+ cells while SB431542 induced more CD34+/38+ cells. Also SB431542 treated cells showed higher colony formation capacity. SR1 increased the expression of c-Myc, HOXB4 and SALL4 while SB431542 seemed to inhibit HOXB4 expression and increase SALL4.Together all, this study introduced a new ex vivo culture setting for further medical application of HSCs. Our data showed simultaneous use of these two small molecules can provide appropriate outcome for HSCs transplantation includes both of engraftment and repopulation.

Sign in / Sign up

Export Citation Format

Share Document