The comparative effect of magnetic activated cell sorting, density gradient centrifugation and swim up on assisted reproduction outcomes, sperm DNA fragmentation, and aneuploidy: A systematic review and meta-analysis

Meta Gene ◽  
2019 ◽  
Vol 22 ◽  
pp. 100607
Author(s):  
Yousef Daneshmandpour ◽  
Fariba Pashazadeh ◽  
Fereshteh Ansari ◽  
Hossein Hosseinifard ◽  
Mohammad Nouri ◽  
...  
2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
R Higashiyama ◽  
M Kishimoto ◽  
S Komure ◽  
S Mizuta ◽  
K Kitaya ◽  
...  

Abstract Study question To analyze whether microfluidic sperm selection (MSS) by ZyMōt™ improves sperm DNA fragmentation rate and embryonic development compared to density gradient centrifugation with swim-up (DGCS). Summary answer MSS by ZyMōt™ selects sperm for clinical use with less DNA damage significantly compared to DGCS. What is known already Conventional sperm preparation methods, such as density gradient centrifugation and the swim-up method utilize centrifugation during processing, may damage the sperm. MSS may allow for improved selection of normal sperm compared with conventional sperm preparation as it yields sperm with a lower DNA fragmentation rate. However, there are few clinical studies by sibling oocytes study compared to DGCS. Study design, size, duration This prospective study was performed between March 2020 and May 2020 at a reproductive center. All patients involved gave written consent, and institutional review board approval was granted. A total of 575 metaphase II oocytes were collected from 49 cycles. Wife’s age was 34.7 ± 3.9 years old. Raw sperm concentration and motile sperm concentration was 63.1 ± 78.7M/mL, and 41.6 ± 67.7M/mL, respectively. Participants/materials, setting, methods Patients who performed ART for the first or second time were divided into two groups according to MSS and DGCS. Sperm DNA fragmentation rate (SDFR) and motile sperm concentration were compered between MSS and DGCS. SDFR was measured by sperm chromatin structure assay (SCSA) using a flow cytometer. Sibling oocytes were randomized into MSS-IVF, DGCS-IVF, MSS-ICSI, and DGCS-ICSI. Rate of two pronuclear (2PN) oocytes, blastocysts development, and good-quality blastocysts were compared between each group. Main results and the role of chance SDFR was 13.5 ± 11.8% for raw semen. SDFR was significantly lower after MSS (3.6 ± 4.1%) than that for raw semen and after DGCS (17.4 ± 14.8%) (P < 0.01). Motile sperm concentration after MSS (19.0 ± 28.3M/mL) was significantly higher after than after DGCS (15.4 ± 15.3M/mL) (P < 0.01). The number of IVF performed was 145 for MSS and 132 for DGCS. IVF results (MSS vs DGCS) were 2PN rate (73.1% vs 72.0%), blastocysts development rate (65.3% vs 55.4%), and good quality blastocysts rate (43.2% vs 34.9%). The number of ICSI performed was 149 for MSS and 149 for DGCS. ICSI results (MSS vs DGCS) were 2PN rate (77.9% vs 79.2%), blastocysts development rate (68.8% vs 65.8%), and good quality blastocysts rate (35.8% vs 30.6%). No significant difference was observed between MSS and DGCS for each parameter both IVF and ICSI. Limitations, reasons for caution The participants were limited to those who collected semen of 2mL or more and motile sperm concentration of above 1M/mL, because semen sample needed to be divided to MSS and DGCS. Wider implications of the findings: This is the first study to conducted in sibling oosytes study with MSS and DGCS, in both IVF and ICSI. MSS is effective in collecting sperm with less DNA damage compared to DGCS. Motile sperm concentration after using MSS is sufficient to perform IVF as well as DGCS. Trial registration number Not applicable


Andrology ◽  
2017 ◽  
Vol 5 (4) ◽  
pp. 776-782 ◽  
Author(s):  
T. S. Berteli ◽  
M. G. Da Broi ◽  
W. P. Martins ◽  
R. A. Ferriani ◽  
P. A. Navarro

2017 ◽  
Vol 9 (13) ◽  
pp. 73
Author(s):  
Khairul Osman ◽  
Noraina Mohd Bakri ◽  
Farah Hanan Fathihah Jaffar ◽  
Nurul Atikah Osman ◽  
Siti Fatimah Ibrahim

Conventional and novel spermatozoa separation techniques have their own strength and weaknesses. Both are widely used in assisted reproductive technologies (ARTs). In this study, combinations of two separation methods against single separation methods were evaluated. Freshly ejaculated semen was collected and divided into five groups; control, density gradient centrifugation (DGC), magnetic activated cell sorting (MACS), DGC-MACS and MACS-DGC; based on the sequence of separation. Evaluation of spermatozoa kinematics, viability and acrosome status for each group were done by computer assisted semen analysis (CASA), hypo-osmotic swelling test (HOST) and chlorotetracycline (CTC) staining respectively. Results show that DGC yield the fastest spermatozoa velocity and highest percentage of viable spermatozoa fraction compared to other groups. However, they also produced a high percentage of acrosome-reacted spermatozoa. Spermatozoa isolated through DGC-MACS shows the highest percentage of non-capacitated spermatozoa. However, spermatozoa isolated through DGC-MACS yields slower spermatozoa velocity population and lower percentage of viable sperm compared to spermatozoa isolated through DGC. Isolation of spermatozoa through MACS-DGC yields slower population of spermatozoa velocity with a lower percentage of non-capacitated spermatozoa compared to spermatozoa isolated through DGC-MACS. DGC separation and its combination with MACS had yield different spermatozoa quality. This distinct characteristic is likely suitable for in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) procedure respectively.


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