Effect of biosal®, deltamethrin and lambda-cyhalothrin on the activity of GOT, GPT and total protein contents in two fodder pests Hermolaus modestus and Hermolaus ocimumi

The assessment of the comparative effect of biosal (phytopesticide), deltamethrin, and lambda-cyhalothrin (pyrethroids) were made against two fodder pests, Hermolaus modestus and Hermolaus ocimumi by filter paper impregnation method. The activity of total protein contents, GPT (glutamic-pyruvic transaminase) and GOT (glutamic oxaloacetate transaminase) were affected in Hermolaus modestus and Hermolaus ocimumi against biosal, deltamethrin, and lambda cyhalothrin. The activity of total protein contents in H. modestus was 31.053%, 4.607%, and 24.575%, against biosal, deltamethrin, and lambda-cyhalothrin, respectively. The activity of total protein contents was observed as 24.202%, 15.25%, and 56.036% against deltamethrin, lambda-cyhalothrin, and biosal, respectively in H. ocimumi. The activity of GOT was observed as 98.675% for biosal 33.95% for deltamethrin and 83.619% for lambda-cyhalothrin in H. modestus. The GOT activity was estimated in H. ocimumi as 78.831%, 47.645%, and 71.287% against biosal, deltamethrin, and lambda-cyhalothrin, respectively. The efficacy of GPT enzyme against biosal, deltamethrin, and lambda-cyhalothrin was calculated as 89.26%, 73.07%, and 47.58%, respectively in H. modestus. The H. ocimumi showed GPT activity as 77.58% for biosal, 68.84% for deltamethrin, and 52.67% for lambda-cyhalothrin, respectively.

Comparative effect of fertilization on cocoyam and component crops on Cocoyam-based intercropping systems in Uyo, Southeastern Nigeria

Field experiment was conducted at University of Uyo Teaching, research farm during the 2018 and 2019 cropping seasons to assess the effect of fertilization on cocoyam-based intercropping systems. The experiment was laid out in a randomized complete block design with three replications. Treatments were six fertilizers rates - 0kg/ha, NPK200kg/ha, NPK400kg/ha, PM2.5t/ha, PM5.0t/ha, Mixture (NPK200kg/ha + PM2.5t/ha) and six crop mixtures - Sole Cocoyam, Sole Melon, Sole Maize, Cocoyam + Maize, Cocoyam + Melon and Cocoyam + Melon + Maize). Result analysed with analysis of variance revealed PM2.5t/ha, NPK400kg/ha and Mixture (NPK200kg/ha + PM2.5t/ha) gave higher plant height, leaf area, stem girth in sole cocoyam, maize and melon respectively. PM2.5t/ha favoured leaf area, stem girths of cocoyam, maize with cocoyam + maize, cocoyam + melon and cocoyam + melon + maize crop mixtures. PM5.0t/ha gave higher values of corm, cormels, cormels weight, cormels number and cormels length in both sole cocoyam and mixtures (cocoyam + melon, cocoyam + maize, cocoyam + melon + maize) in both seasons respectively. PM2.5t/ha gave higher values of maize grain yield with cocoyam + maize, cocoyam + melon + maize crop mixtures. PM5.0t/ha and NPK400kg/ha gave higher melon seed values with cocoyam + melon and cocoyam + Melon + maize. Conclusively, it is recommended that application of organic fertilizer promotes higher yields and yield components in both sole and crop mixtures of cocoyam and maize respectively.

Physical, morphological and storage stability of Clove oil nanoemulsion based delivery system

Clove oil based Nanoemulsions (NE) were prepared ultrasonically using Tween 80 and soy lecithin as synthetic and natural surfactants, respectively. The developed NEs were characterized for various parameters (particle size, polydispersity index, zeta potential, morphology, viscosity, colour, turbidity and pH) and the comparative effect of both the surfactants at variable levels (oil:tween 80-1:1, 1:2, 1:3 and 1:4 and oil: soy lecithin- 1:1, 1:1.5 and 1:2) was assessed. It was found that the type of surfactant and oil to surfactant ratio significantly affected particle size and stability of NEs. The NE prepared using tween 80 @1:3 had smallest average droplet diameter (40.9 nm). The formulated NEs were stored at 25 °C and 4 °C and analyzed for turbidity, pH and phase separation up to 90 days. Results revealed that the type and concentration of the surfactant significantly influenced the particle size and stability of NEs. NEs prepared using tween 80 were found to be more viscous than those prepared with soy lecithin. The prepared clove oil NEs have important implication to be used as a natural delivery system to increase the shelf life of food products.