Localization of 20α-hydroxysteroid dehydrogenase mRNA in mouse brain by in situ hybridization

2004 ◽  
Vol 125 (1-2) ◽  
pp. 143-146 ◽  
Author(s):  
G. Pelletier ◽  
V. Luu-The ◽  
S. Li ◽  
F. Labrie
Keyword(s):  
In Situ Hybridization ◽  
Mouse Brain ◽  
Hydroxysteroid Dehydrogenase

scholarly journals Localization of type 1 17beta-hydroxysteroid dehydrogenase mRNA and protein in syncytiotrophoblasts and invasive cytotrophoblasts in the human term villi

2000 ◽  
Vol 165 (2) ◽  
pp. 217-222 ◽  
Author(s):  
M Bonenfant ◽  
PR Provost ◽  
R Drolet ◽  
Y Tremblay
Keyword(s):  
In Situ Hybridization ◽  
Hydroxysteroid Dehydrogenase ◽  
Binding Activity ◽  
Placental Lactogen ◽  
Specific Expression ◽  
P450 Aromatase ◽  
Chain Cleavage ◽  
Extravillous Cytotrophoblasts

The 17beta-hydroxysteroid dehydrogenases (17beta-HSDs) play a key role in the synthesis of sex steroids. The hallmark of this family of enzymes is the interconversion, through their oxydoreductive reactivity at position C17, of 17-keto- and 17beta-hydroxy-steroids. Because this reaction essentially transforms steroids having low binding activity for the steroid receptor to their more potent 17beta-hydroxysteroids isoforms, it is crucial to the control of the physiological activities of both estrogens and androgens. The human placenta produces large amounts of progesterone and estrogens throughout pregnancy. The placental type 1 17beta-HSD enzyme (E17beta-HSD) catalyzes the reduction of the low activity estrogen, estrone, into the potent estrogen, estradiol. We studied the cell-specific expression of type 1 17beta-HSD in human term placental villous tissue by combining in situ hybridization to localize type 1 17beta-HSD mRNA with immunohistochemistry using an antibody against human placental lactogen, a trophoblast marker. Immunolocalization of E17beta-HSD was also performed. To ascertain whether other steroidogenic enzymes are present in the same cell type, cytochrome P450 cholesterol side-chain cleavage (P450scc), P450 aromatase, and type 1 3beta-hydroxysteroid dehydrogenase (3beta-HSD) were also localized by immunostaining. Our results showed that the syncytium is the major steroidogenic unit of the fetal term villi. In fact, type 1 17beta-HSD mRNA and protein, as well as P450scc, P450 aromatase, and 3beta-HSD immunoreactivities were found in these cells. In addition, our results revealed undoubtedly that extravillous cytotrophoblasts (CTBs), e.g. those from which cell columns of anchoring villous originate, also express the type 1 17beta-HSD gene. However, CTBs lying beneath the syncytial layer, e.g. those from which syncytiotrophoblasts develop, contained barely detectable amounts of type 1 17beta-HSD mRNA as determined by in situ hybridization. These findings, along with those from other laboratories confirm the primordial role of the syncytium in the synthesis of steroids during pregnancy. In addition, our results indicate for the first time that CTBs differentiating along the invasive pathway contain type 1 17beta-HSD mRNA.

scholarly journals Ontogenesis of 3β-Hydroxysteroid Dehydrogenase Δ5-Δ4 Isomerase in the Rat Ovary as Studied by Immunocytochemistry and in Situ Hybridization

1993 ◽  
Vol 48 (2) ◽  
pp. 226-234 ◽  
Author(s):  
Christian Juneau ◽  
Eric Dupont ◽  
Van Luu-The ◽  
Fernand Labrie ◽  
Georges Pelletier
Keyword(s):  
In Situ Hybridization ◽  
Hydroxysteroid Dehydrogenase ◽  
Rat Ovary

Developmental expression of somatostatin in mouse brain. II. In situ hybridization

1990 ◽  
Vol 53 (1) ◽  
pp. 26-39 ◽  
Author(s):  
Caterina Bendotti ◽  
Christine Hohmann ◽  
Gianluigi Forloni ◽  
Roger Reeves ◽  
Joseph T. Coyle ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Mouse Brain ◽  
Developmental Expression

scholarly journals Localization of Type 5 17β-Hydroxysteroid Dehydrogenase, 3β-Hydroxysteroid Dehydrogenase, and Androgen Receptor in the Human Prostate by in Situ Hybridization and Immunocytochemistry

Endocrinology ◽  
1999 ◽  
Vol 140 (3) ◽  
pp. 1481-1491 ◽  
Author(s):  
Mohamed El-Alfy ◽  
Van Luu-The ◽  
Xiao-Fang Huang ◽  
Louise Berger ◽  
Fernand Labrie ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Androgen Receptor ◽  
Hydroxysteroid Dehydrogenase ◽  
Human Prostate

Anatomical characterization of the neuropeptide S system in the mouse brain by in situ hybridization and immunohistochemistry

2011 ◽  
Vol 519 (10) ◽  
pp. 1867-1893 ◽  
Author(s):  
Stewart D. Clark ◽  
Dee M. Duangdao ◽  
Stefan Schulz ◽  
Li Zhang ◽  
Xiaobin Liu ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Mouse Brain ◽  
Neuropeptide S ◽  
Anatomical Characterization
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