Frequency of micronuclei in peripheral blood lymphocytes from subjects occupationally exposed to low levels of ionizing radiation

Chromosome Aberrations in Peripheral Blood Lymphocytes of Croatian Hospital Staff Occupationally Exposed to Low Levels of Ionising RadiationMedical staff is an occupational group exposed to different agents suspected to induce genetic damage. Among them ionising radiation is the most studied. Cytogenetic analysis of human chromosomes in peripheral lymphocytes allows direct detection of mutation in somatic cells. This study investigated the cytogenetic effects of low-level ionising x-radiation in 48-hour peripheral blood lymphocyte cultures sampled from 765 hospital staff occupationally exposed to several agents known or suspected to induce chromosome damage and compared them with 200 control subjects. The exposed subjects were divided in eight (8) groups according to their specialities and job titles. The exposed groups manifested an increase in all types of chromosome aberrations. Acentric fragments were the most frequent chromosome-type aberration. Dicentric chromosomes were statistically significant only in urologists/gynaecologists. Age and smoking significantly influenced the incidence of dicentrics in the exposed groups. The frequency of ring chromosomes was low in all exposed groups (range: 0-2), and none were found in the control group. These findings indicate the importance of periodic medical checkups of hospital staff occupationally exposed to low doses of ionising radiation. The purpose is to create an individual cytogenetic register, where changes could evidence individual risks.

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Duration of the hidden chromosome instability persistence in peripheral blood lymphocytes of persons occupationally exposed to ionizing radiation

Reports of the National Academy of Sciences of Ukraine ◽

10.15407/dopovidi2015.07.143 ◽

2015 ◽

pp. 143-149

Author(s):

M.A. Pilinskaya ◽

◽

S.S. Dibskiy ◽

Ye.B. Dibskaya ◽

L.I. Shvayko ◽

...

Keyword(s):

Ionizing Radiation ◽

Peripheral Blood ◽

Chromosome Instability ◽

Peripheral Blood Lymphocytes ◽

Blood Lymphocytes ◽

Occupationally Exposed

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Cytogenetic monitoring of peripheral blood lymphocytes from medical radiation professionals occupationally exposed to low-dose ionizing radiation

Mutation Research/Genetic Toxicology and Environmental Mutagenesis ◽

10.1016/j.mrgentox.2021.503370 ◽

2021 ◽

Vol 867 ◽

pp. 503370

Author(s):

Xue-Lei Tian ◽

Xue Lu ◽

Tian-Jing Cai ◽

Yu-Min Lyu ◽

Mei Tian ◽

...

Keyword(s):

Ionizing Radiation ◽

Peripheral Blood ◽

Low Dose ◽

Peripheral Blood Lymphocytes ◽

Blood Lymphocytes ◽

Medical Radiation ◽

Occupationally Exposed ◽

Cytogenetic Monitoring

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Cytogenetic analysis of peripheral blood lymphocytes of hospital staff occupationally exposed to low doses of ionizing radiation

Toxicology and Industrial Health ◽

10.1177/0748233710365693 ◽

2010 ◽

Vol 26 (5) ◽

pp. 273-280 ◽

Cited By ~ 18

Author(s):

Ayşe Eken ◽

Ahmet Aydın ◽

Onur Erdem ◽

Cemal Akay ◽

Hatice Tuba Sanal ◽

...

Keyword(s):

Ionizing Radiation ◽

Peripheral Blood ◽

Chromosome Instability ◽

Hospital Staff ◽

Peripheral Blood Lymphocytes ◽

Control Group ◽

Blood Lymphocytes ◽

Significant Difference ◽

Cbmn Assay ◽

Occupationally Exposed

Ionizing radiation is known to induce mutations and cell transformations, predominantly by causing single-strand and double-strand DNA breakage, thereby leading to chromosome instability and carcinogenesis. The aim of this study was to evaluate genotoxic effects in hospital staff exposed to low-dose ionizing radiation in comparison with a selected control group, by using the cytokinesis-blocked micronucleus (CBMN) and sister chromatid exchange (SCE) tests in peripheral blood lymphocytes. The study included 40 exposed radiology staff and 30 control subjects. The frequency of micronuclei (MN) was significantly increased in radiation-exposed groups compared with control persons (p < 0.05). The frequency of SCE did not show any significant difference in the exposed individuals in comparison to the controls. Our results showed that low-level chronic occupational exposure to ionizing radiation causes an increase of MN frequency in chromosomes, even though the absorbed doses were below the permissible limits. Our studies indicate that the CBMN assay is considered to be sensitive test in contrast to SCE analysis to evaluate chromosomal damage induced by ionizing radiation.

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