Stud-breeding work in sheep breeding based on cytogenetic monitoring

The article presents the results of the cytogenetic monitoring in the breeding, selection and reproduction of sheep in the ecological conditions of Ukraine. A cytogenetic analysis of sheep with low and high vigor and different levels of fertility was carried out. In the ecological conditions of Ukraine, the individual level of chromosomal abnormalities in all the studied animals of the Tsigai breed and prekos is within the natural background. No translocations were found. Among the violations, such as single and paired fragments, hypo- and hyperploidy (mainly hypoploidy), polyploidy were encountered. The maximum average level of chromosomal abnormalities was found in local lambs (5.5 ±1.73%). The minimum average level of chromosomal abnormalities (2.0 ±1.41%) was recorded in lambs with high growth intensity. Among the structural changes, mutations of the chromosomal type prevailed in the 3-year-old group of rams - 0.46%, in the 8-year-old group - 0.59%. Chromatid disturbances were 0.37% and 0.34%, respectively. The average level of chromosomal abnormalities in rams by groups was 0.84 ±0.14 and 0.93 ±0.13, respectively. In the group of ewes with low fertility (n = 4), the level of chromosomal abnormalities was 3.5%, which is lower than ewes (n = 7) with high fertility by 0.79%.

Complex cytogenetic abnormalities in chronic myeloid leukemia resulting in early progression to blast crisis: a case report

Introduction BCR-ABL1, resulting from t(9;22), is the oncogenic driver of chronic myeloid leukemia and the therapeutic target of the disease. Molecular studies have been the gold standard modality for patient assessment since the advent of tyrosine kinase inhibitor therapy. In spite of that, there are cytogenetic abnormalities that can render the disease unresponsive to conventional therapy, thus making cytogenetics an important component of patient management guidelines. Case presentation We present a case of a Tajik, Afghan patient with chronic myeloid leukemia with del(6)(q23.3q27), t(9;22)(q34;q11.2), monosomy 11, monosomy 12, and marker chromosome who, despite having typical clinical and hematological disease with initial response to therapy, progressed to blast crisis very early and thus required special interventions. Conclusion Cytogenetic monitoring is an important pillar in the management of patients with chronic myeloid leukemia that cannot be ignored. It should therefore be a part of patient management not only during diagnosis but also during management. We present an unusual cytogenetic abnormality in a patient with chronic myeloid leukemia that resulted in early disease progression.

Breeding and Economic Aspects of Cytogenetic Screening Studies of Pigs Qualified for Reproduction

Cytogenetic monitoring allows the identification and early removal of pigs affected by inherited karyotype defects from breeding herds. These abnormalities cause developmental anomalies, considerably reducing the fertility (by several dozen to 100%) and performance parameters of breeding herds, resulting in substantial financial losses. This mainly concerns reciprocal translocations, typical of pigs, which are highly prevalent (about 0.46%), generally occur de novo, and normally result in low breeding soundness of the carriers. Due to the potential spontaneous occurrence of chromosomal aberrations and the rapid spread of these genetic defects in the population, especially under artificial insemination conditions, it is necessary to perform routine karyotype screening of animals qualified for reproduction. The cytogenetic screening program for young boars, carried out using continually refined diagnostic techniques, permits a precise and reliable karyotype assessment, identification of chromosomal abnormalities, and formulation of specific selection guidelines.

Genotoxicity test methods - a tool for DNA and chromosome damage biomonitoring

Nowadays, life is highly influenced by intense growth of various industries, high levels of pollution, and other environmental factors with harmful effects on human health. Therefore, cytogenetic monitoring is essential for detection of changes in the structure of chromosomes, which occur because of the effects of various genotoxic agents. In this review, we shall apprize the theoretical and experimental aspects of several tests for assessment of genotoxicity in humans such as Micronucleus assay, Comet assay, Chromosomal aberrations assessment and Sister chromatid exchanges analysis. These methods are accepted by the World Health Organization as standard tests for genotoxicological screening in humans. The methods are sensitive and confirm the cellular genotoxic effects of various genotoxicants.

FEATURES OF THE KARYOTYPE OF WHITE-HEADED COWS OF UKRAINIAN BREED

The White-Headed Ukrainian breed of cattle belongs to breeds of a dairy direction of productivity and belongs to small disappearing breeds. According to calculations, in accordance with the FAO recommendations, the White-Headed Ukrainian breed of cattle is at risk. The aim of the work was to establish the karyotype variability of White-Headed Ukrainian cows, whose animals belong to closed gene pool herds. Cytogenetic monitoring showed that the quantitative chromosome abnormalities, in particular aneuploidy, in White-Headed Ukrainian cows of two groups according to the purpose of the cow (selection nucleus and production group) did not exceed the spontaneous level of this variability (1.5-8.3%) and was 6.0% and 7.0% respectively. A multiple increase in the number of chromosomes (polyploidy) was not detected. The average value of metaphase plates with asynchronous differences in the centromeric regions of chromosomes was 1.0% and corresponded to the level of this variability characteristic of the species Bos taurus. The frequency of structural chromosome abnormalities (chromosomal breaks) in animals of the two groups was 2.3% and 2.0%, respectively, which corresponds to the norm characteristic of cattle with spontaneous mutagenesis. The results of the micronucleus test showed that the proportion of lymphocytes with micronuclei in animals of the two groups was in the range of 4.8 - 5.0%, dinuclear lymphocytes 5.6-6.4 %, and the proportion of dividing cells (mitotic index) was 2.8-3.8 %. The frequency of cells with aneuploidy in cows of the Ukrainian Black-Spotted dairy breed of this farm was established higher than the spontaneous level. The difference between the mean values on this basis in cows of White-Headed Ukrainian and Ukrainian Black-Spotted dairy breeds was statistically significant at P> 0.99. In cows of the Ukrainian Black-Spotted dairy breed under conditions of intensive use, an increase in the frequency of metaphases with aneuploidy was observed, which was not manifested in cows of the White-Headed Ukrainian breed.

Identification and certification of Bactrian and Arvan camels of Kazakh population using DNA technology// FUNDAMENTAL AND APPLIED SCIENTIFIC RESEARCH: сб.науч.тр./ OEAPS Inc.; редкол.: Флора Бертран (отв.ред.) [и др.]. - Берлин, Германия : OEAPS Inc., 2018. - С. 44-56.

Kazakhstan is the center where Bactrians and Dromedaries can be raised, and hybridization between them has become widespread. Genetic resources of interspecies camel hybrids, raised in Kazakhstan, are represented by 30 generations.The most highly valuable in the conditions of Central Asia and Kazakhstan are transboundary camel breeds such as Kazakh Bactrian, Turkmen and Kazakh Dromedary, as well as new generations of camels Arada, Bainar and Baitur.At pure breeding of Kazakh Bactrian, genetic peculiarities are not taken into account, in view of the poor knowledge of this issue. To maintain the biodiversity, molecular genetic monitoring of the state of camel populations is required. Cytogenetic monitoring and certification are one of the promising areas for reliable camel genome assessment.Deep determination of Kazakh dairy camelsallele poolwas not carried out in Kazakhstan before. Genetic studies to optimize structure, identification and certification of valuable genotypes, as well as systematization of genetic resources in dairy camel breeding in Kazakhstan are urgent task.The aim of the study is identification, systematization and certification of genetic resources of Bactrian and Dromedary camels of Kazakh population using DNA technology. Genetic analysis of the typing results of the camels studied was carried out by 7 and 8 loci of DNA microsatellites. The material for the studies was DNA samples isolated from histological samples. The microsatellite loci were selected in accordance with the recommendations of the European Society of Genetics (FAO/ISAG).Identification and certification of Arvan and Kazakh Bactrian camels’ genetic resources using the microsatellite analysis of their genotype was carried outfor the first time. Breeding significance of Bactrian and Arvan camels of Kazakh population using DNA technologies was studied and determined. The obtained results of the study allowed evaluate camels at the level of the allelic profile.

CYTOGENETIC INDICATION OF MUTAGENIC EFFECT OCCURRING IN «MOTHER – CHILD» DYADS INFLUENCED OF ALUMINUM-PRODUCING ENTERPRISES

It is important to conduct cytogenetic monitoring to assess the level and dynamics of the frequency of cells with genome damage in parents and their children, formed in response to the impact of aluminum. Material and methods. In dyads «mother – child», the content of aluminum in urine was determined and a morphological evaluation of changes in buccal epitheliocytes was carried out. Results. It was found that in children and adults the concentration of aluminum in urine was 6,8 times higher than in the comparison groups and up to 5,5 times the reference level. The content of aluminum in urine in children is 1,3 times higher than in adults. The concentration of aluminum in urine (more than 0,0065 mg/dm3) is justified as an exposure marker. In children with a concentration of aluminum in urine at a level of 0,032–0,040 mg/dm3 and higher, the indicator indicators of genetic disorders are: an increase in the frequency of multinucleated cells, an integral index of cytogenetic action, and the frequency of cells with karyorexis. In adults with an aluminum concentration in urine of 0,021–0,033 mg/dm3 and higher markers of genetic disorders is the increased frequency of micronucleated cells and cells with karyorexis. Conclusion. In the dyads «mother – child», the spectrum and degree of severity of deviations in the indicators of genetic disorders in children relative to those of their mothers is growing.

Legitimacy of systematic karyotype evaluation of cattle qualified for reproduction

Cytogenetic studies enable the identification and early elimination from breeding of animals affected by hereditary karyotype defects. These defects cause developmental disorders and a significant decrease of fertility parameters (from a few dozen to 100%) and productivity of breeding herds, and consequently measurable financial losses. Due to the potential possibility of spontaneous occurrence of chromosomal aberrations and rapid spreading of these genetic defects in the population, especially in the conditions of the use of artificial insemination, it is necessary to carry out regular karyotype control of breeding animals. Cytogenetic monitoring of cattle qualified for reproduction guarantees the systematic identification of all karyotype abnormalities carriers, which, causing the decrease of fertility or sterility, determine the low reproductive performance of animals affected by these defects. The cytogenetic control system, implemented by the use of constantly perfected cytomolecular techniques, enables a very precise, reliable evaluation of karyotype and diagnosis of chromosomal abnormalities, as well as formulation of specific recommendations for culling.