scholarly journals Fetomaternal microchimerism and genetic diagnosis: On the origins of fetal cells and cell-free fetal DNA in the pregnant woman

2021 ◽  
pp. 108399
Author(s):  
Margit Rosner ◽  
Thomas Kolbe ◽  
Markus Hengstschläger
Keyword(s):  
Pregnant Woman ◽  
Genetic Diagnosis ◽  
Fetal Cells ◽  
Fetal Dna ◽  
Cell Free Fetal Dna

Examination of Fetal Cells and Cell-Free Fetal DNA in Maternal Blood for Fetal Gender Determination

2004 ◽  
Vol 58 (1) ◽  
pp. 57-60 ◽  
Author(s):  
Xiao Xi Zhao ◽  
Nobuhiro Suzumori ◽  
Yasuhiko Ozaki ◽  
Takeshi Sato ◽  
Kaoru Suzumori
Keyword(s):  
Maternal Blood ◽  
Gender Determination ◽  
Fetal Cells ◽  
Fetal Dna ◽  
Fetal Gender ◽  
Cell Free Fetal Dna

Cell-free fetal DNA in the non-pregnant woman with thyroid disease disappeared after surgery

2008 ◽  
Vol 397 (1-2) ◽  
pp. 101-102
Author(s):  
Miho Tanigawa ◽  
Hiroshi Miyoshi ◽  
Osamu Samura ◽  
Keizo Sugino ◽  
Naoya Fujito ◽  
...  
Keyword(s):  
Pregnant Woman ◽  
Thyroid Disease ◽  
Fetal Dna ◽  
Cell Free Fetal Dna

Progress in prenatal genetic diagnosis: Using cell-free fetal DNA in maternal blood

2013 ◽  
Vol 21 (2) ◽  
pp. 84-90 ◽  
Author(s):  
Kerry Oxenford ◽  
Madhavi Karunaratna ◽  
Melissa Hill ◽  
Sally Taffinder ◽  
Professor Lyn Chitty
Keyword(s):  
Genetic Diagnosis ◽  
Maternal Blood ◽  
Fetal Dna ◽  
Prenatal Genetic Diagnosis ◽  
Cell Free Fetal Dna

Diagnostic Value of Non-Invasive Prenatal Screening of β-thalassemia by Cell Free Fetal DNA and Fetal NRBC

2019 ◽  
Vol 19 (2) ◽  
pp. 105-111
Author(s):  
Nadia Shafei ◽  
Mohammad Saeed Hakhamaneshi ◽  
Massoud Houshmand ◽  
Siavash Gerayeshnejad ◽  
Fardin Fathi ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Multiple Displacement Amplification ◽  
Diagnostic Value ◽  
Diagnostic Methods ◽  
Beta Thalassemia ◽  
Non Invasive ◽  
Fetal Dna ◽  
Prenatal Diagnostic ◽  
Cell Free Fetal Dna ◽  
Invasive Techniques

Background: Beta thalassemia is a common disorder with autosomal recessive inheritance. The most prenatal diagnostic methods are the invasive techniques that have the risk of miscarriage. Now the non-invasive methods will be gradually alternative for these invasive techniques. Objective: The aim of this study is to evaluate and compare the diagnostic value of two non-invasive diagnostic methods for fetal thalassemia using cell free fetal DNA (cff-DNA) and nucleated RBC (NRBC) in one sampling community. Methods: 10 ml of blood was taken in two k3EDTA tube from 32 pregnant women (mean of gestational age = 11 weeks), who themselves and their husbands had minor thalassemia. One tube was used to enrich NRBC and other was used for cff-DNA extraction. NRBCs were isolated by MACS method and immunohistochemistry; the genome of stained cells was amplified by multiple displacement amplification (MDA) procedure. These products were used as template in b-globin segments PCR. cff-DNA was extracted by THP method and 300 bp areas were recovered from the agarose gel as fetus DNA. These DNA were used as template in touch down PCR to amplify b-globin gen. The amplified b-globin segments were sequenced and the results compared with CVS resul. Results: The data showed that sensitivity and specificity of thalassemia diagnosis by NRBC were 100% and 92% respectively and sensitivity and specificity of thalassemia diagnosis by cff-DNA were 100% and 84% respectively. Conclusion: These methods with high sensitivity can be used as screening test but due to their lower specificity than CVS, they cannot be used as diagnostic test.

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