Species identification and in vitro antifungal susceptibility testing of Aspergillus section Nigri strains isolated from otomycosis patients

2018 ◽  
Vol 28 (2) ◽  
pp. 279-284 ◽  
Author(s):  
Z. Kamali Sarwestani ◽  
S.J. Hashemi ◽  
S. Rezaie ◽  
M. Gerami Shoar ◽  
S. Mahmoudi ◽  
...  
Keyword(s):  
Species Identification ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Aspergillus Section Nigri ◽  
In Vitro Antifungal Susceptibility ◽  
Aspergillus Section

scholarly journals Species Identification and Antifungal Susceptibility Patterns of Species Belonging to Aspergillus Section Nigri

2009 ◽  
Vol 53 (10) ◽  
pp. 4514-4517 ◽  
Author(s):  
Laura Alcazar-Fuoli ◽  
Emilia Mellado ◽  
Ana Alastruey-Izquierdo ◽  
Manuel Cuenca-Estrella ◽  
Juan L. Rodriguez-Tudela
Keyword(s):  
Phylogenetic Analysis ◽  
Species Identification ◽  
Antifungal Susceptibility ◽  
Morphological Features ◽  
Correct Classification ◽  
Paradoxical Effect ◽  
Aspergillus Section Nigri ◽  
Susceptibility Patterns ◽  
Aspergillus Section

ABSTRACT A phylogenetic analysis was performed for 34 Aspergillus strains belonging to section Nigri. Molecular methods allowed for the correct classification into three different clades (A. niger, A. tubingensis, and A. foetidus). Correlation with in vitro itraconazole susceptibility distinguished the following three profiles: susceptible, resistant, and showing a paradoxical effect. A number of different species whose morphological features resemble those of A. niger showed unusual MICs to itraconazole that have never been described for the Aspergillus genus.

Vaginitis Due To Pichia fermentans in a Patient Affected by Endometrial Cancer: A Novel Case Report

2021 ◽  
Vol 17 ◽  
Author(s):  
Zarifeh Adampour ◽  
Malihe Hasanzadeh ◽  
Hossein Zarrinfar ◽  
Maryam Nakhaei ◽  
Monika Novak Babič
Keyword(s):  
Endometrial Cancer ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Female Genital Tract ◽  
Antifungal Susceptibility Testing ◽  
Vein Thrombosis ◽  
Microbial Infections ◽  
Life Threatening ◽  
In Vitro Antifungal Susceptibility

Introduction: Endometrial cancer is one of the most common malignancies of the female genital tract, which can be serious or life-threatening. Microbial infections can be one of the underlying causes of this type of cancer. Case Presentation: The present study describes the isolation of Pichia fermentans (Candida firmentaria var. firmentaria) from the vaginal secretions of a 61-year-old woman affected by endometrial cancer. She reported abdominal pain and vaginal discharge for 3 months, and had a history of diabetes, hypertension, Deep Vein Thrombosis (DVT), and Acute Myeloid Leukemia (AML). The isolated yeast was identified based on nuclear ribosomal internal transcribed spacer (ITS1-ITS2 rDNA) sequence analysis. The in vitro antifungal susceptibility testing showed a higher effect for ketoconazole against P. fermentans than fluconazole, itraconazole and voriconazole. Conclusion: Correct differentiation between P. fermentans and other yeast should be considered. The in vitro antifungal susceptibility testing is recommended for rare yeast, and will help the physicians in providing the best treatment.

scholarly journals Standardized Method for In Vitro Antifungal Susceptibility Testing of Candida albicansBiofilms

2001 ◽  
Vol 45 (9) ◽  
pp. 2475-2479 ◽  
Author(s):  
Gordon Ramage ◽  
Kacy Vande Walle ◽  
Brian L. Wickes ◽  
José L. López-Ribot
Keyword(s):  
Antimicrobial Agents ◽  
Susceptibility Testing ◽  
Colorimetric Assay ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Phenotypic Traits ◽  
Salt Reduction ◽  
Treatment Regimens ◽  
In Vitro Antifungal Susceptibility

ABSTRACT Candida albicans is implicated in many biomaterial-related infections. Typically, these infections are associated with biofilm formation. Cells in biofilms display phenotypic traits that are dramatically different from those of their free-floating planktonic counterparts and are notoriously resistant to antimicrobial agents. Consequently, biofilm-related infections are inherently difficult to treat and to fully eradicate with normal treatment regimens. Here, we report a rapid and highly reproducible microtiter-based colorimetric assay for the susceptibility testing of fungal biofilms, based on the measurement of metabolic activities of the sessile cells by using a formazan salt reduction assay. The assay was used for in vitro antifungal susceptibility testing of severalC. albicans strains grown as biofilms against amphotericin B and fluconazole and the increased resistance of C. albicans biofilms against these antifungal agents was demonstrated. Because of its simplicity, compatibility with a widely available 96-well microplate platform, high throughput, and automation potential, we believe this assay represents a promising tool for the standardization of in vitro antifungal susceptibility testing of fungal biofilms.

In vitro antifungal susceptibility testing of fungi in patients with onychomycosis

2020 ◽  
Vol 33 (3) ◽  
Author(s):  
Mohammed Abu El‐Hamd ◽  
Manar Ibrahim Abd Elhameed ◽  
Mona Fattouh Mohamed Shalaby ◽  
Ramadan Saleh
Keyword(s):  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
In Vitro Antifungal Susceptibility
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