Critical contribution of macrophage scavenger receptor 1 to the uptake of nanostructured DNA by immune cells

2021 ◽  
pp. 102386
Author(s):  
Keisuke Umemura ◽  
Shozo Ohtsuki ◽  
Makoto Nagaoka ◽  
Kosuke Kusamori ◽  
Takao Inoue ◽  
...  
Keyword(s):  
Immune Cells ◽  
Scavenger Receptor ◽  
Macrophage Scavenger Receptor

scholarly journals Phthalide derivative CD21 attenuates tissue plasminogen activator-induced hemorrhagic transformation in ischemic stroke by enhancing macrophage scavenger receptor 1-mediated DAMP (peroxiredoxin 1) clearance

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Dong-Ling Liu ◽  
Zhi Hong ◽  
Jing-Ying Li ◽  
Yu-Xin Yang ◽  
Chu Chen ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Brain Injury ◽  
Middle Cerebral Artery ◽  
Combined Treatment ◽  
Scavenger Receptor ◽  
Hemorrhagic Transformation ◽  
Microglial Cells ◽  
Peroxiredoxin 1 ◽  
Macrophage Scavenger Receptor ◽  
Tissue Plasminogen

Abstract Background Hemorrhagic transformation (HT) is a critical issue in thrombolytic therapy in acute ischemic stroke. Damage-associated molecular pattern (DAMP)-stimulated sterile neuroinflammation plays a crucial role in the development of thrombolysis-associated HT. Our previous study showed that the phthalide derivative CD21 attenuated neuroinflammation and brain injury in rodent models of ischemic stroke. The present study explored the effects and underlying mechanism of action of CD21 on tissue plasminogen activator (tPA)-induced HT in a mouse model of transient middle cerebral artery occlusion (tMCAO) and cultured primary microglial cells. Methods The tMCAO model was induced by 2 h occlusion of the left middle cerebral artery with polylysine-coated sutures in wildtype (WT) mice and macrophage scavenger receptor 1 knockout (MSR1−/−) mice. At the onset of reperfusion, tPA (10 mg/kg) was intravenously administered within 30 min, followed by an intravenous injection of CD21 (13.79 mg/kg/day). Neuropathological changes were detected in mice 3 days after surgery. The effect of CD21 on phagocytosis of the DAMP peroxiredoxin 1 (Prx1) in lysosomes was observed in cultured primary microglial cells from brain tissues of WT and MSR1−/− mice. Results Seventy-two hours after brain ischemia, CD21 significantly attenuated neurobehavioral dysfunction and infarct volume. The tPA-infused group exhibited more severe brain dysfunction and hemorrhage. Compared with tPA alone, combined treatment with tPA and CD21 significantly attenuated ischemic brain injury and hemorrhage. Combined treatment significantly decreased Evans blue extravasation, matrix metalloproteinase 9 expression and activity, extracellular Prx1 content, proinflammatory cytokine mRNA levels, glial cells, and Toll-like receptor 4 (TLR4)/nuclear factor κB (NF-κB) pathway activation and increased the expression of tight junction proteins (zonula occludens-1 and claudin-5), V-maf musculoaponeurotic fibrosarcoma oncogene homolog B, and MSR1. MSR1 knockout significantly abolished the protective effect of CD21 against tPA-induced HT in tMCAO mice. Moreover, the CD21-induced phagocytosis of Prx1 was MSR1-dependent in cultured primary microglial cells from WT and MSR1−/− mice, respectively. Conclusion The phthalide derivative CD21 attenuated tPA-induced HT in acute ischemic stroke by promoting MSR1-induced DAMP (Prx1) clearance and inhibition of the TLR4/NF-κB pathway and neuroinflammation.

scholarly journals Human mesangial cells express inducible macrophage scavenger receptor

1999 ◽  
Vol 56 (2) ◽  
pp. 440-451 ◽  
Author(s):  
Xiong Z. Ruan ◽  
Zac Varghese ◽  
Stephen H. Powis ◽  
John F. Moorhead
Keyword(s):  
Mesangial Cells ◽  
Scavenger Receptor ◽  
Human Mesangial Cells ◽  
Macrophage Scavenger Receptor

Iron loading increases cholesterol accumulation and macrophage scavenger receptor I expression in THP-1 mononuclear phagocytes

Metabolism ◽  
2005 ◽  
Vol 54 (4) ◽  
pp. 453-459 ◽  
Author(s):  
Pavel J. Kraml ◽  
Richard L. Klein ◽  
Yan Huang ◽  
Alena Nareika ◽  
Maria F. Lopes-Virella
Keyword(s):  
Scavenger Receptor ◽  
Mononuclear Phagocytes ◽  
Iron Loading ◽  
Cholesterol Accumulation ◽  
Macrophage Scavenger Receptor

scholarly journals Macrophage Scavenger Receptor A Mediates Adhesion to Apolipoproteins A-I and E

Biochemistry ◽  
2009 ◽  
Vol 48 (50) ◽  
pp. 11858-11871 ◽  
Author(s):  
Claudine Neyen ◽  
Annette Plüddemann ◽  
Pietro Roversi ◽  
Benjamin Thomas ◽  
Lei Cai ◽  
...  
Keyword(s):  
Scavenger Receptor ◽  
Macrophage Scavenger Receptor ◽  
Scavenger Receptor A

scholarly journals Endocytosis by macrophages: interplay of macrophage scavenger receptor-1 and LDL receptor-related protein-1

Haematologica ◽  
2019 ◽  
Vol 105 (3) ◽  
pp. e133-e137 ◽  
Author(s):  
Eelke P. Béguin ◽  
Małgorzata A. Przeradzka ◽  
Esmée F.J. Janssen ◽  
Henriët Meems ◽  
Magdalena Sedek ◽  
...  
Keyword(s):  
Scavenger Receptor ◽  
Ldl Receptor ◽  
Related Protein ◽  
Macrophage Scavenger Receptor
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