Bacterial type IV secretion systems: a tool for DNA transfer into mammalian cells

2009 ◽  
Vol 25 ◽  
pp. S35
Author(s):  
E. Fernández-González ◽  
H.D. de Paz ◽  
C. Dehio ◽  
F.J. Sangari ◽  
M. Llosa
Keyword(s):  
Mammalian Cells ◽  
Type Iv Secretion ◽  
Dna Transfer ◽  
Secretion Systems ◽  
Type Iv ◽  
Type Iv Secretion Systems ◽  
Bacterial Type

scholarly journals Two type IV secretion systems with different functions in Burkholderia cenocepacia K56-2

Microbiology ◽  
2009 ◽  
Vol 155 (12) ◽  
pp. 4005-4013 ◽  
Author(s):  
Ruifu Zhang ◽  
John J. LiPuma ◽  
Carlos F. Gonzalez
Keyword(s):  
Type Iv Secretion ◽  
Burkholderia Cenocepacia ◽  
Target Cells ◽  
Secretion Systems ◽  
Type Iv ◽  
Plasmid Mobilization ◽  
Type Iv Secretion Systems ◽  
Derivative Plasmid ◽  
Chromosome Ii ◽  
Bacterial Type

Bacterial type IV secretion systems (T4SS) perform two fundamental functions related to pathogenesis: the delivery of effector molecules to eukaryotic target cells, and genetic exchange. Two T4SSs have been identified in Burkholderia cenocepacia K56-2, a representative of the ET12 lineage of the B. cepacia complex (Bcc). The plant tissue watersoaking (Ptw) T4SS encoded on a resident 92 kb plasmid is a chimera composed of VirB/D4 and F-specific subunits, and is responsible for the translocation of effector(s) that have been linked to the Ptw phenotype. The bc-VirB/D4 system located on chromosome II displays homology to the VirB/D4 T4SS of Agrobacterium tumefaciens. In contrast to the Ptw T4SS, the bc-VirB/D4 T4SS was found to be dispensable for Ptw effector(s) secretion, but was found to be involved in plasmid mobilization. The fertility inhibitor Osa did not affect the secretion of Ptw effector(s) via the Ptw system, but did disrupt the mobilization of a RSF1010 derivative plasmid.

scholarly journals Bacterial Type IV secretion systems: versatile virulence machines

2012 ◽  
Vol 7 (2) ◽  
pp. 241-257 ◽  
Author(s):  
Daniel E Voth ◽  
Laura J Broederdorf ◽  
Joseph G Graham
Keyword(s):  
Type Iv Secretion ◽  
Secretion Systems ◽  
Type Iv ◽  
Type Iv Secretion Systems ◽  
Bacterial Type

scholarly journals The versatile bacterial type IV secretion systems

2003 ◽  
Vol 1 (2) ◽  
pp. 137-149 ◽  
Author(s):  
Eric Cascales ◽  
Peter J. Christie
Keyword(s):  
Type Iv Secretion ◽  
Secretion Systems ◽  
Type Iv ◽  
Type Iv Secretion Systems ◽  
Bacterial Type

VirB2 and VirB5 proteins: specialized adhesins in bacterial type-IV secretion systems?

2008 ◽  
Vol 16 (9) ◽  
pp. 409-413 ◽  
Author(s):  
Steffen Backert ◽  
Remi Fronzes ◽  
Gabriel Waksman
Keyword(s):  
Type Iv Secretion ◽  
Secretion Systems ◽  
Type Iv ◽  
Type Iv Secretion Systems ◽  
Bacterial Type

scholarly journals BIOGENESIS, ARCHITECTURE, AND FUNCTION OF BACTERIAL TYPE IV SECRETION SYSTEMS

2005 ◽  
Vol 59 (1) ◽  
pp. 451-485 ◽  
Author(s):  
Peter J. Christie ◽  
Krishnamohan Atmakuri ◽  
Vidhya Krishnamoorthy ◽  
Simon Jakubowski ◽  
Eric Cascales
Keyword(s):  
Type Iv Secretion ◽  
Secretion Systems ◽  
Type Iv ◽  
Type Iv Secretion Systems ◽  
And Function ◽  
Bacterial Type

scholarly journals Functional interactions between type IV secretion systems involved in DNA transfer and virulence

Microbiology ◽  
2005 ◽  
Vol 151 (11) ◽  
pp. 3505-3516 ◽  
Author(s):  
Héctor D. de Paz ◽  
Félix J. Sangari ◽  
Silvia Bolland ◽  
Juan M. García-Lobo ◽  
Christoph Dehio ◽  
...  
Keyword(s):  
Type Iv Secretion ◽  
Dna Transfer ◽  
Secretion Systems ◽  
Animal Cells ◽  
Protein Levels ◽  
Bacterial Pathogenicity ◽  
Functional Interactions ◽  
Type Iv ◽  
Type Iv Secretion Systems ◽  
Coupling Protein

This paper reports an analysis of the functional interactions between type IV secretion systems (T4SS) that are part of the conjugative machinery for horizontal DNA transfer (cT4SS), and T4SS involved in bacterial pathogenicity (pT4SS). The authors' previous work showed that a conjugative coupling protein (T4CP) interacts with the VirB10-type component of the T4SS in order to recruit the protein–DNA complex to the transporter for conjugative DNA transfer. This study now shows by two-hybrid analysis that conjugative T4CPs also interact with the VirB10 element of the pT4SS of Agrobacterium tumefaciens (At), Bartonella tribocorum (Bt) and Brucella suis (Bs). Moreover, the VirB10 component of a cT4SS (protein TrwE of plasmid R388) could be partially substituted by that of a pT4SS (protein TrwE of Bt) for conjugation. This result opens the way for the construction of hybrid T4SS that deliver DNA into animal cells. Interestingly, in the presence of part of the Bs T4SS the R388 T4SS protein levels were decreased and R388 conjugation was strongly inhibited. Complementation assays between the Trw systems of R388 and Bt showed that only individual components from the so-called ‘core complex’ could be exchanged, supporting the concept that this core is the common scaffold for the transport apparatus while the other ‘peripheral components' are largely system-specific.

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